Review



pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)
    Pcdna™6.2/C‐Emgfp‐Gw/Topo™ Only (Empty Vector [Ev], Control), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pmc06590123-74-18-10
    Average 90 stars, based on 1 article reviews
    pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Selection:

    Article Title: Structural basis of THC analog activity at the Cannabinoid 1 receptor
    Article Snippet: .. Antibiotics including blasticidin (5 μg/mL) and ZeocinTM (20 μg/mL) were introduced as selection agents to create stable cell lines expressing the pcDNATM4/TO a mammalian expression vector that encodes the appropriate CB 1 sequence (ThermoFisher Scientific, UK). .. Whilst Geneticin® (G-418 sulphate, 200 μg/mL) was used to select for cells containing the pcDNA™ 3.1 mammalian expression vector (Invitrogen™), encoding venus-mGsi and venus-β-arrestin2.

    Stable Transfection:

    Article Title: Structural basis of THC analog activity at the Cannabinoid 1 receptor
    Article Snippet: .. Antibiotics including blasticidin (5 μg/mL) and ZeocinTM (20 μg/mL) were introduced as selection agents to create stable cell lines expressing the pcDNATM4/TO a mammalian expression vector that encodes the appropriate CB 1 sequence (ThermoFisher Scientific, UK). .. Whilst Geneticin® (G-418 sulphate, 200 μg/mL) was used to select for cells containing the pcDNA™ 3.1 mammalian expression vector (Invitrogen™), encoding venus-mGsi and venus-β-arrestin2.

    Expressing:

    Article Title: Structural basis of THC analog activity at the Cannabinoid 1 receptor
    Article Snippet: .. Antibiotics including blasticidin (5 μg/mL) and ZeocinTM (20 μg/mL) were introduced as selection agents to create stable cell lines expressing the pcDNATM4/TO a mammalian expression vector that encodes the appropriate CB 1 sequence (ThermoFisher Scientific, UK). .. Whilst Geneticin® (G-418 sulphate, 200 μg/mL) was used to select for cells containing the pcDNA™ 3.1 mammalian expression vector (Invitrogen™), encoding venus-mGsi and venus-β-arrestin2.

    Plasmid Preparation:

    Article Title: Structural basis of THC analog activity at the Cannabinoid 1 receptor
    Article Snippet: .. Antibiotics including blasticidin (5 μg/mL) and ZeocinTM (20 μg/mL) were introduced as selection agents to create stable cell lines expressing the pcDNATM4/TO a mammalian expression vector that encodes the appropriate CB 1 sequence (ThermoFisher Scientific, UK). .. Whilst Geneticin® (G-418 sulphate, 200 μg/mL) was used to select for cells containing the pcDNA™ 3.1 mammalian expression vector (Invitrogen™), encoding venus-mGsi and venus-β-arrestin2.

    Article Title: Tuberculosis antigen cassettes
    Article Snippet: The vector can also be of the MAXBACTM complete baculovirus expression system (Invitrogen, San Diego, Calif.), which can be used for protein production in insect cells. .. The vector can also be pcDNA I or pcDNA3 (Invitrogen, San Diego, Calif.), which may be used for protein production in mammalian cells such as Chinese hamster ovary (CHO) cells. .. The vector can be expression vectors or systems to produce protein by routine techniques and readily available starting materials including Sambrook et al., Molecular Cloning and Laboratory Manual, Second Ed., Cold Spring Harbor (1989).

    Sequencing:

    Article Title: Structural basis of THC analog activity at the Cannabinoid 1 receptor
    Article Snippet: .. Antibiotics including blasticidin (5 μg/mL) and ZeocinTM (20 μg/mL) were introduced as selection agents to create stable cell lines expressing the pcDNATM4/TO a mammalian expression vector that encodes the appropriate CB 1 sequence (ThermoFisher Scientific, UK). .. Whilst Geneticin® (G-418 sulphate, 200 μg/mL) was used to select for cells containing the pcDNA™ 3.1 mammalian expression vector (Invitrogen™), encoding venus-mGsi and venus-β-arrestin2.

    other:

    Article Title: m6A sites in the coding region trigger translation-dependent mRNA decay.
    Article Snippet: The PCR product was inserted into pcDNA 5 /FRT/TO (Invitrogen, #V652020) by standard cloning procedures using BamHI and HindIII restriction sites.

    Article Title: Expression in mammalian cells with Gaussia luciferase signal peptide
    Article Snippet: It was done by transfecting pcDNATM6/TR vector (Life Technologies) into these lines.

    Article Title: Phosphoinositide- and Collybistin-Dependent Synaptic Clustering of Gephyrin
    Article Snippet: For Geph expression in HEK GPHN -/- cells, the construct mScarlet-IRES-mEGFP-Geph in pcDNATM 5/FRT/TO (Thermo Fischer) was cloned via overlap extension PCR followed by Gibson cloning using the following plasmids as templates: pAAV-hSyn-mScarlet (gift from Karl Deisseroth; Addgene plasmid #131001; http://n2t.net/addgene:131001 ; RRID:Addgene_131001); pAAV-EF1a-mCherry-IRES-Flpo (gift from Karl Deisseroth; Addgene plasmid #55634; http://n2t.net/addgene:55634 ; RRID:Addgene_55634); pAAV-hSyn-fDIO-mEGFP-Gphn_P1 (Addgene plasmid #194978; http://n2t.net/addgene:194978 ; RRID:Addgene_194978).

    Article Title: Improving Split-HaloTag through Computational Protein Engineering
    Article Snippet: Sequences were cloned in the pcDNATM5/FRT (Thermo Fisher Scientific Inc.) vector.

    Article Title: The RAB27A effector SYTL5 regulates mitophagy and mitochondrial metabolism
    Article Snippet: To generate U2OS stably expressing SYTL5-EGFP, U2OS FlpIn TRex cells and U2OS FlpIn TRex_mScarlet-RAB27A were co-transfected with 0.1 μg pcDNATM5/FRT/TO_SYTL5-EGFP and 0.9 μg pOG44 Flp recombinase expression vector using LipofectamineTM 2000 (Thermo Fisher Scientific #11668019) according to the manufactureŕs instructions.

    Article Title: Expression in mammalian cells with Gaussia luciferase signal peptide
    Article Snippet: The a1-PDX coding sequence was subcloned into the following mammalian expression vectors: a) pCEP-Pu (pCEP-Pu/AC7 (Kohfeldt et al, 2007), based on pCEP4 (Invitrogen)—the most popular vector for episomal expression); b) pcDNATM4/HisMax (Invitrogen) vector, an advanced version of pcDNATM3.1, which is a popular vector for stable expression; c) pRTS-1 (Bornkamm et al., 2005), a kind gift of Prof. Dirk Eick, German Research Centre for Environmental Health (Munich). pCEP-Pu vector was chosen for transient transfection of the pCEP-Pu:PLA2R1 stable cell line. pcDNATM4/HisMax vector contained a Zeocin® antibiotic resistance gene, whereas pRTS-1 had a hygromycin resistance gene.



    Similar Products

    90
    Vigene Biosciences empty vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/pmc10834511-52-6-13
    Average 90 stars, based on 1 article reviews
    empty vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Genecopoeia empty preceiver-lv105 vector (ev) as a control
    Empty Preceiver Lv105 Vector (Ev) As A Control, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/lv105/pmc10351194-42-19-29
    Average 90 stars, based on 1 article reviews
    empty preceiver-lv105 vector (ev) as a control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Sino Biological empty vector ev
    Empty Vector Ev, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCMV3-N-FLAG+Negative+Control+Vector/bio_rxiv__2023__07__10__547766-45-2-13
    Average 93 stars, based on 1 article reviews
    empty vector ev - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Vigene Biosciences empty-vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/ppr0660169-57-5-11
    Average 90 stars, based on 1 article reviews
    empty-vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Genecopoeia control empty vector (ev
    Control Empty Vector (Ev, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vect+control/pmc11072547-195-15-22
    Average 90 stars, based on 1 article reviews
    control empty vector (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    80
    OriGene empty vector ev controls
    Empty Vector Ev Controls, supplied by OriGene, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCas-Guide-Puro-CRISPRa+gene+activation+vector/pm34449924-51-9-16
    Average 80 stars, based on 1 article reviews
    empty vector ev controls - by Bioz Stars, 2026-10
    80/100 stars
      Buy from Supplier

    90
    Millipore p3xflag-cmv empty vector (ev) control
    P3xflag Cmv Empty Vector (Ev) Control, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/p3xflag+cmv+10/pm29208611-90-10-17
    Average 90 stars, based on 1 article reviews
    p3xflag-cmv empty vector (ev) control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)
    Pcdna™6.2/C‐Emgfp‐Gw/Topo™ Only (Empty Vector [Ev], Control), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pmc06590123-74-18-10
    Average 90 stars, based on 1 article reviews
    pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 1 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Journal: Arthritis research & therapy

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    doi: 10.1186/s13075-017-1388-8

    Figure Lengend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Article Snippet: Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs.

    Techniques: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay