Review



empty vector ev control  (Vector Biolabs)


Bioz Verified Symbol Vector Biolabs is a verified supplier
Bioz Manufacturer Symbol Vector Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 16 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis."

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    Journal: Arthritis research & therapy

    doi: 10.1186/s13075-017-1388-8

    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Figure Legend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Techniques Used: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay

    Related Articles

    Knock-Out:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    In Vitro:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Control:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Expressing:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Marker:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Plasmid Preparation:

    Article Title: The Histone Methyltransferases EHMT1 and EHMT2 Repress the Expression of Genes Related to Excitability and Cell Death in Oligodendrocyte Progenitors.
    Article Snippet: To induce differentiation, cells were cultured in Sato medium without mitogens and supplemented with 3,3′,5- Triiodo- L- thyronine sodium salt (T3, 60 nM, Sigma, T5516) for 72 h. .. To knock out Ehmt1 and Ehmt2 in vitro, immunopanned OPCs were obtained from Ehmt1fl/fl;Ehmt2fl/fl mice and transduced at an MOI of 10 either with a control adenoviral vector expressing a red fluorescent protein as a fluorescent marker (Ad- RFP), or with an adenoviral vector expressing a codon- improved Cre recombinase together with the RFP reporter (Ad- RFP- iCre; Vector Biolabs 1660; 1774) for 6 h in a tissue culture incubator. ..

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Construct:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Immunoprecipitation:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Western Blot:

    Article Title: Age-Dependent Histone Deacetylase 3 Regulation by βA3/A1-Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age-Related Macular Degeneration.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 15 of 18 Ad- RFP (Vector Biolabs, Cat # 1660) or Ad- Cryba1- RFP (Vector Biolabs, customized) constructs at 105 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti- RFP beads, followed by immunoblot for HDAC3. ..

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Virus:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Recombinant:

    Article Title: Vaccines for in vivo expression of nucleic acids and methods of using the same
    Article Snippet: .. Ad5/RFP (Ad-RFP), the virus used in this particle vector, was from Vector biolab and is a recombinant human type 5 adenovirus expressing Red Fluorescent Protein (RFP) under the control of a CMV promoter. .. The plasmid, 2019-nCov_pcDNA3.1(+)-P2A-eGFP, is from Genscript.

    Binding Assay:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Over Expression:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..

    Infection:

    Article Title: Age‐Dependent Histone Deacetylase 3 Regulation by βA3 / A1 ‐Crystallin and Inositol Hexaphosphate in Retinal Pigmented Epithelial Cells Reveals a Novel Pathway in Age‐Related Macular Degeneration
    Article Snippet: All measurements were performed in triplicate, data analyzed, and the IC50 value was calculated using GraphPad Prism (version 6.0, GraphPad Software Inc; Watson et al. , ). .. To ascertain HDAC3 and βA3/A1‐crystallin binding in overexpression conditions, Cryba1 fl/fl RPE explants were infected with Ad‐RFP (Vector Biolabs, Cat # 1660) or Ad‐ Cryba1 ‐RFP (Vector Biolabs, customized) constructs at 10 5 vg/mL for 24 h. The RPE lysates were prepared, and immunoprecipitation was performed using anti‐RFP beads, followed by immunoblot for HDAC3. ..



    Similar Products

    90
    Genecopoeia empty preceiver-lv105 vector (ev) as a control
    Empty Preceiver Lv105 Vector (Ev) As A Control, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/lv105/pmc10351194-42-19-29
    Average 90 stars, based on 1 article reviews
    empty preceiver-lv105 vector (ev) as a control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Vigene Biosciences empty vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/pmc10834511-52-6-13
    Average 90 stars, based on 1 article reviews
    empty vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    93
    Sino Biological empty vector ev
    Empty Vector Ev, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCMV3-N-FLAG+Negative+Control+Vector/bio_rxiv__2023__07__10__547766-45-2-13
    Average 93 stars, based on 1 article reviews
    empty vector ev - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    90
    Vigene Biosciences empty-vector control (ev
    Empty Vector Control (Ev, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vector+control++ev/ppr0660169-57-5-11
    Average 90 stars, based on 1 article reviews
    empty-vector control (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Genecopoeia control empty vector (ev
    Control Empty Vector (Ev, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/empty+vect+control/pmc11072547-195-15-22
    Average 90 stars, based on 1 article reviews
    control empty vector (ev - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    80
    OriGene empty vector ev controls
    Empty Vector Ev Controls, supplied by OriGene, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pCas-Guide-Puro-CRISPRa+gene+activation+vector/pm34449924-51-9-16
    Average 80 stars, based on 1 article reviews
    empty vector ev controls - by Bioz Stars, 2026-10
    80/100 stars
      Buy from Supplier

    90
    Millipore p3xflag-cmv empty vector (ev) control
    P3xflag Cmv Empty Vector (Ev) Control, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/p3xflag+cmv+10/pm29208611-90-10-17
    Average 90 stars, based on 1 article reviews
    p3xflag-cmv empty vector (ev) control - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control)
    Pcdna™6.2/C‐Emgfp‐Gw/Topo™ Only (Empty Vector [Ev], Control), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/pmc06590123-74-18-10
    Average 90 stars, based on 1 article reviews
    pcdna™6.2/c‐emgfp‐gw/topo™ only (empty vector [ev], control) - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 1 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Journal: Arthritis research & therapy

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    doi: 10.1186/s13075-017-1388-8

    Figure Lengend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Article Snippet: Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs.

    Techniques: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay