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Structured Review

Merck KGaA immobilon-p pdvf membrane
Immobilon P Pdvf Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/electrotransfer+buffer/pvdf+membrane/pmc06502797-242-15-18
Average 90 stars, based on 1 article reviews
immobilon-p pdvf membrane - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Nucleic Acid Electrophoresis:

Article Title: Hypidone hydrochloride (YL-0919) ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation
Article Snippet: Protein concentrations were determined using BCA Protein Assay Kits (Solarbio, Beijing, China). .. Equal amounts of protein separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were transferred to polyvinylidene fluoride membranes (MerckMillipore, Burlington, MA, USA), which were subsequently blocked with 5% skim milk (LABLED, Beijing, China) at room temperature for 2 hours. .. The membranes were incubated with the following primary antibodies at 4°C overnight: rabbit anti-AQP-4 (1:1000, Immunoway, Plano, TX, USA, Cat# YT0292, RRID: AB_3076280), rabbit anti-pNrf2 (Phospho-Ser40) (1:1000, Immunoway, Cat# YP1659, RRID: AB_3076282), and mouse anti-β-actin (1:2000, Zenbio, Chengdu, China, Cat# 200068-8F10, RRID: AB_2722710).

other:

Article Title: Minute amounts of helicase-deficient truncated RECQL4 are sufficient for DNA replication
Article Snippet: Protein concentrations were measured with Pierce BCA assay kit and, unless otherwise indicated, 25μg protein extract was separated on pre-cast NuPAGETM BOLT 8% Bis-Tris polyacrylamide gels (Invitrogen) and transferred onto Immobilon-P PVDF membranes (Merck Millipore).

Article Title: Development of an anti-CDH15/M-cadherin monoclonal antibody Ca 15 Mab-1 for flow cytometry, immunoblotting, and immunohistochemistry
Article Snippet: The fitting binding isotherms (vertical axis, GeoMean; horizontal axis, mAb concentration) determined the dissociation constant ( K D ) values to built-in one-side binding models of GraphPad Prism 6 (GraphPad Software, Inc. La Jolla, CA, USA).

Article Title: Iron overload and oxidative stress participated in zinc oxide nanoparticles-induced blood-brain barrier dysfunction.
Article Snippet: The separated proteins were transferred onto Polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Ireland), and then which were incubated with 5 % bovine serum albumin (BSA) or skim milk in Tris-buffered saline containing 0.1 % Tween 20 (TBST) for blocking.

SDS Page:

Article Title: Mechanistic exploration of hexokinase 2 and metabolism in diabetic cardiomyopathy
Article Snippet: .. Equal amounts of protein (20 μg/lane) were separated by 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-PSQ Blotting Membranes; 0.2 μm; Merck KGaA). .. Following 1-hour blocking at room temperature with 5% skimmed milk, the membranes were incubated overnight at 4°C with the following antibodies: Anti-GAPDH mouse monoclonal (cat. no. 60004-1-Ig; 1:5,000; Proteintech Group, Inc.), anti-microsomal glutathione S-transferase 1 (MGST1) rabbit monoclonal (cat. no. A0880; 1:500; ABclonal Biotech Co., Ltd.), anti-HK2 rabbit polyclonal (1:1,000; cat. no. A0994; ABclonal Biotech Co., Ltd,) and anti-Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-Containing Subunit Gamma (PIK3C2G) polyclonal antibody (cat. no. 25904-1-AP; 1:2,000; Proteintech Group, Inc.).

Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming
Article Snippet: Protein concentration was measured using the BCA protein assay kit (P0010, Beyotime). .. Equal amounts of protein (30 μg) were separated by SDS-PAGE and transferred onto PVDF membranes (ISEQ00010, Merck millipore). .. Membranes were blocked with universal antibody dilution buffer (U3510, Sigma-Aldrich) for 1 h and incubated overnight at 4 °C with primary antibodies against GPX4, ALOX12, NOX4, and FAP-α (1:1000).

Membrane:

Article Title: Mechanistic exploration of hexokinase 2 and metabolism in diabetic cardiomyopathy
Article Snippet: .. Equal amounts of protein (20 μg/lane) were separated by 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-PSQ Blotting Membranes; 0.2 μm; Merck KGaA). .. Following 1-hour blocking at room temperature with 5% skimmed milk, the membranes were incubated overnight at 4°C with the following antibodies: Anti-GAPDH mouse monoclonal (cat. no. 60004-1-Ig; 1:5,000; Proteintech Group, Inc.), anti-microsomal glutathione S-transferase 1 (MGST1) rabbit monoclonal (cat. no. A0880; 1:500; ABclonal Biotech Co., Ltd.), anti-HK2 rabbit polyclonal (1:1,000; cat. no. A0994; ABclonal Biotech Co., Ltd,) and anti-Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-Containing Subunit Gamma (PIK3C2G) polyclonal antibody (cat. no. 25904-1-AP; 1:2,000; Proteintech Group, Inc.).



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