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electrotransfer tank  (Bio-Rad)


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    Structured Review

    Bio-Rad electrotransfer tank
    Electrotransfer Tank, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+buffer/Buffer+Tank/pmc11971999-72-8-19
    Average 96 stars, based on 228 article reviews
    electrotransfer tank - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with pre-cooled transfer buffer (25 mM Tris, 0.2 M glycine, 10% methanol) at 100 V for 30 min. For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. For detection of PDEδ, loading control β -actin, as well as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti- β -actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature.

    Article Title: Targeting a Glutamic Acid in PDEδ with Fluoromethyl-Aryl Electrophiles Impairs K-Ras Signaling.
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with precooled transfer buffer (25mMTris, 0.2M glycine, 10%methanol) at 100 V for 30 min. Membranes were blocked with Intercept Blocking Buffer (LI-CORBiosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-PDEδ (Invitrogen, PA522008, RRID: AB_11154288, 1:500) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LICOR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. The sample from each treatment condition was distributed equally into ten tubes and subjected to heating at different temperatures in the MasterCycler EpGradient S (Eppendorf SE, DE).

    Control:

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with pre-cooled transfer buffer (25 mM Tris, 0.2 M glycine, 10% methanol) at 100 V for 30 min. For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. For detection of PDEδ, loading control β -actin, as well as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti- β -actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with pre-cooled transfer buffer (25 mM Tris, 0.2 M glycine, 10% methanol) at 100 V for 30 min. For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. For detection of PDEδ, loading control β -actin, as well as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti- β -actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature.

    Article Title: Targeting a Glutamic Acid in PDEδ with Fluoromethyl-Aryl Electrophiles Impairs K-Ras Signaling.
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with precooled transfer buffer (25mMTris, 0.2M glycine, 10%methanol) at 100 V for 30 min. Membranes were blocked with Intercept Blocking Buffer (LI-CORBiosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-PDEδ (Invitrogen, PA522008, RRID: AB_11154288, 1:500) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LICOR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. The sample from each treatment condition was distributed equally into ten tubes and subjected to heating at different temperatures in the MasterCycler EpGradient S (Eppendorf SE, DE).

    Incubation:

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with pre-cooled transfer buffer (25 mM Tris, 0.2 M glycine, 10% methanol) at 100 V for 30 min. For detection of vinculin as loading control, membranes were blocked with Intercept Blocking Buffer (LI-COR Biosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-vinculin (Sigma-Aldrich, V9131, RRID: AB_477629, 1:5000) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LI-COR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. For detection of PDEδ, loading control β -actin, as well as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti- β -actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)-conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature.

    Article Title: Targeting a Glutamic Acid in PDEδ with Fluoromethyl-Aryl Electrophiles Impairs K-Ras Signaling.
    Article Snippet: .. Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Thermo Fisher Scientific, #88518, 0.45 μm) using a wet-tank blotting system (Bio-Rad) with precooled transfer buffer (25mMTris, 0.2M glycine, 10%methanol) at 100 V for 30 min. Membranes were blocked with Intercept Blocking Buffer (LI-CORBiosciences, #927-70001) for 1 h at room temperature, incubated with the primary antibody anti-PDEδ (Invitrogen, PA522008, RRID: AB_11154288, 1:500) overnight at 4 °C in blocking buffer, then washed with PBS-T (PBS with 0.1% Tween-20) five times and incubated with IRDye 800CW-conjugated secondary antibody (LICOR Biosciences, #926-32210, RRID: AB_621842, 1:5000) in blocking buffer for 1 h at room temperature with protection from light. .. The sample from each treatment condition was distributed equally into ten tubes and subjected to heating at different temperatures in the MasterCycler EpGradient S (Eppendorf SE, DE).

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer.
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS– PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000 g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS–PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    other:

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology.
    Article Snippet: Samples were then cooled to room temperature and centrifuged at 100,000×g for 20min at 4 °C (Beckman Coulter Optima MAX-XP, TLA-120.1 rotor).

    Article Title: Age-dependent topoisomerase I depletion alters recruitment of rDNA silencing complexes
    Article Snippet: Protein concentration was measured by Bradford Assay with a UV-visible spectrophotometer (BioMate3, Thermo Fisher Scientific).

    Article Title: Targeting a Glutamic Acid in PDEδ with Fluoromethyl-Aryl Electrophiles Impairs K‑Ras Signaling
    Article Snippet: Soluble fractions were separated from denatured proteins by ultracentrifugation at 100,000 g and 4 °C for 25 min (Beckman Coulter Optima MAX-XP, with TLA-120.1 rotor).

    Protein Concentration:

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer.
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS– PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000 g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS–PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    Bradford Assay:

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer.
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS– PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000 g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS–PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..

    SDS Page:

    Article Title: Nutrient requirements of organ-specific metastasis in breast cancer.
    Article Snippet: Cells were washed once in PBS and scraped into RIPA lysis buffer containing protease and phosphatase inhibitors (5871, Cell Signaling Technology), rocked for 15 min at 4 °C, and insoluble material was sedimented by centrifugation at 21,000g for 10 min at 4 °C. .. Protein concentration was determined via Bradford Assay, and samples were mixed with LDS sample buffer (NP0008, Thermo Fisher Scientific) and 2.5% 2-mercaptoethanol and then incubated at 95 °C for 5 min. Proteins were resolved by SDS– PAGE and then transferred onto nitrocellulose membranes using a wet tank transfer system (Bio-Rad). ..



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