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tris buffered saline tbs  (Thermo Fisher)


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    Thermo Fisher tris buffered saline tbs
    Tris Buffered Saline Tbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+buffer/TRIS-buffered+saline+(TBS/pm17106881-35-12-36
    Average 96 stars, based on 1 article reviews
    tris buffered saline tbs - by Bioz Stars, 2026-10
    96/100 stars

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    Saline:

    Article Title: Large-scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation.
    Article Snippet: .. Cells were washed once with Tris-buffered saline (TBS) and fixed with 4% paraformaldehyde (PFA, methanol-free) (Catalog #043368.9M; Thermo Fisher Scientific) for 15 min at room temperature (RT). .. After three washes with TBS, cells were permeabilized with 0.1% Triton X-100 (Catalog #9036-19-5; Sigma) in TBS for 10 min, followed by another three washes with TBS.

    Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications
    Article Snippet: After deparaffinized and rehydrated, antigen retrieval was performed using antigen unmasking solution (H-3300, Vector Lab), and endogenous peroxidase activity was blocked with BLOXALL Blocking Solution (SP-6000, Vector Lab) for 10 min. .. Following several washes with tris-buffered saline (TBS), tissues were blocked with 2.5% normal horse serum (NHS), incubated with primary antibody (rabbit monoclonal anti-TNF alpha, ab183218, Abcam, 1:500) and matrix metallopeptidase 9 (MMP9) (Mouse monoclonal anti-MMP9, MA5-15886, Invitrogen, 1:200) in 1% NHS for 30 min, treated with biotinylated universal antibody (BUA, anti-mouse/rabbit IgG) for 30 min, and then incubated with avidin-biotin complex (ABC) reagent for 30 min, which are contained in VETASTAIN Elite ABC-HRP kit (PK-7200, Vector Lab). .. The color development was achieved using ImmPACT® DAB Substrate Kit (SK-4105, horseradish peroxidase (HRP), Vector Lab), followed by counterstaining with Hematoxylin QS (H-3404) for 30 s. After being rinsed with DW, stained tissues were mounted with VectaMount QA Mounting Medium (H-5501, Vector Lab) and covered with a coverslip.

    Article Title: Establishment and Characterisation of Two Canine Prostate Cancer Cell Lines with Stem Cell Marker Expression.
    Article Snippet: .. Slides were then washed in Tris-buffered saline (TBS) or TBS with TweenTM (TBS-T) (Thermo Fisher Scientific, Waltham, MA, USA) after a 20 min cooling period when heat-induced epitope retrieval (HIER) was used or immediately following Proteinase K digestion. .. When the VECTASTAIN® Elite® ABC-HRP Kit (Vector Laboratories, Newark, CA, USA) was used, non-specific staining was reduced by incubating the slides in blocking solution—either 5% normal goat serum, 5% bovine serum albumin (BSA) or AnimalFree Blocker® (Vector Laboratories, Newark, CA, USA)—for 30 min at room temperature. https://doi.org/10.3390/ani16050732 Avidin and biotin blocking was performed using an avidin and biotin blocking kit before the slides were incubated overnight at 4 ◦C either with negative control solution or the primary antibody diluted in PBS.

    Article Title: Importin α1 contributes to Venezuelan equine encephalitis virus-induced cell death, but is not required for the capsid-mediated blockage of nucleocytoplasmic trafficking
    Article Snippet: Mouse anti-V5 (BioRad, MCA1360) and rabbit anti-KPNA2 (Bethyl, A300-484A) were used at 1:10000; rabbit anti-CRM1 (Bethyl, A300-469A) was used at 1:2000; anti-KPNA3 (Bethyl, A301-626A) at 1:500, and anti-KPNA4 (Proteintech, 12463-1-AP) at 1:1000. .. Following three 5-min washes in 1x TBST and one in 1x Tris-Buffered Saline (TBS), membranes were incubated with secondary antibodies at 1:2000 (goat anti-mouse IgG, Invitrogen 32430; or goat anti-rabbit IgG, Invitrogen 32460) prepared in 0.5% milk/TBST for 1 h at room temperature. .. After identical washing steps, membranes were developed using the SuperSignalTM West Femto Maximum Sensitivity Substrate (ThermoFisher, 34095) and imaged with a BioRad ChemiDocTM Imaging system.

    Article Title: Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) platform elicits enhanced humoral and cellular immune responses
    Article Snippet: .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20. .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20.

    Article Title: Peri-Microvascular Glycogen and Lactate Regulate Capillary Constrictions and Ischemia Outcome in Mice.
    Article Snippet: Reagent or Resource Source Identifier Antibodies Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 488 Vector Labs Cat. No:Cat. No: DL1174- 1, RRID: N/A Aminopeptidase N/ CD13 Antibody Acris Antibodies GmBH Cat. No:Cat. No: AM26636AF- N, RRID: N/A NG2 Millipore Cat. No:Cat. No: AB5320, RRID: AB_91789 PDGFR- β Abcam Cat. No:Cat. No: ab69506, RRID: AB_1269704 GYS- 1 Cell Signaling Technologies Cat. No:Cat. No: 3893, RRID: AB_2279563 Beta- Actin ThermoFisher Scientific Cat. No: PA1- 183, RRID: AB_2608409 GFAP Abcam Cat. No: ab4674, RRID: AB_304558 MCT1 Polyclonal antibody Proteintech Cat. No: 20139- 1- AP, RRID: AB_2878645 MCT12 Polyclonal antibody Proteintech Cat. No: 20553- 1- AP, RRID: AB_10693621 Hexokinase 2 Polyclonal antibody Proteintech Cat. No: 22029- 1- AP, RRID: AB_11182717 GPR81 Antibody Novus Biologicals Cat. No: NLS2095, RRID: AB_10001932 IV58B6 courtesy of Dr. Otto Baba N/A ESG1A9 courtesy of Dr. Hitoshi Ashida N/A AffiniPure F(ab’)2 Fragment Donkey AntiMouse IgM Jackson Immunoresearch Cat. No: 715- 006- 020, RRID: AB_2340760 Alexa Fluor 488 AffiniPure Donkey Anti- Mouse IgM Jackson Immunoresearch Cat. No: 715- 545- 140, RRID: AB_2340845 Alexa Fluor 488 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11008, RRID: N/A Alexa Fluor 594 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11012, RRID: N/A Alexa Fluor 647 AffiniPure Donkey Anti- Chicken IgY (IgG) (H + L) Jackson Immunoresearch Cat. No: 703- 605- 155, RRID: AB_2340379 Alexa Fluor 568 AffiniPure Donkey Anti- Rat IgG (H + L) Jackson Immunoresearch Cat. No: 712- 575- 153, RRID: AB_3095476 14714159, 2026, 4, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jnc.70430, W iley O nline L ibrary on [17/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 7 of 27Journal of Neurochemistry, 2026 Reagent or Resource Source Identifier Goat anti- Mouse IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21040, RRID: AB_2536527 Goat anti- Rabbit IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21234, RRID: AB_1500696 Chemicals, peptides, and recombinant proteins DAB (1,4- dideoksi1,4- imino- d- arabinitol hydrochloride) Sigma- Aldrich Cat. No: D1542 Sodium D- lactate Sigma- Aldrich Cat. No: 71716 Sodium L- lactate Sigma- Aldrich Cat. No: 71718 Chloral hydrate Sigma- Aldrich Cat. No: 05–2650 Periodic acid Sigma- Aldrich Cat. No: 375810 Schiff 's reagent Merck Cat. No: 3952016 Dimedone (5,5- Dimethyl- 1,3- cyclohexanedione) Sigma- Aldrich Cat. No: D153303 TRIS- buffered saline (TBS, 10X) pH 7.4 ThermoFisher Cat. No: J60764.K2 RIPA Lysis and Extraction Buffer Sigma- Aldrich Cat. No: 89900 Triton- X 100 Sigma- Aldrich Cat. No: X100 Tween- 20 Sigma- Aldrich Cat. No: P1379 Halt Protease and Phosphatase Inhibitor Cocktail (100X) ThermoFisher Cat. No: 78440 NuPAGE Bis- Tris Mini Protein Gels, 4%–12%, 1.0–1.5 mm ThermoFisher Cat. No: NP0322BOX NuPAGE MOPS SDS Running Buffer (20X) ThermoFisher Cat. No: NP0001 PVDF Transfer Membranes ThermoFisher Cat. No: 88518 VECTASHIELD PLUS Antifade Mounting Medium with DAPI (H- 2000) Vector Labs Cat. No: H- 2000, RRID: N/A Experimental models: Organisms/strains Swiss Albino mice Jackson Laboratories Strain #:034608 RRID:IMSR_ JAX:034608 C57Bl/6 wild- type (WT) mice Jackson Laboratories Strain: #:000664 RRID: IMSR_JAX:000664 GYS1Nestin- KO mice Courtesy of Dr. Duran and Dr. Guinovart N/A GYS1Gfap- KO mice Reagent or Resource Source Identifier Software and algorithms ImageJ/Fiji NIH https:// imagej. net/ softw are/ fiji/ Prism- GraphPad Dotmatics https:// www. graph pad. com/ scien tific- softw are/ prism/ www. graph pad. com/ scien tificsoftw are/ prism/ Endnote 20 Clarivate https:// endno te. com/? langu age= en Adobe Photoshop Adobe https:// www. adobe. com/ produ cts/ photo shop. html/ Adobe Illustrator Adobe https:// www. adobe. com/ produ cts/ illus trator. html/ ..

    Article Title: Host tRNA modifications drive efficient translation of influenza A virus genome and impact host antiviral stress responses
    Article Snippet: After sonication, the lysates were centrifugated for 20 minutes at 16.000g, the supernatants were collected, and the protein content was quantified using the BCA Protein Assay Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions. .. Protein lysates were transferred to nitrocellulose membranes, blocked with 5% non-fat dry milk diluted in tris-buffered saline (TBS)-tween and immunoblotted with antibodies against ELP3 (ThermoFisher, 1:200 dilution), anti-puromycin antibody (clone 12D10; Sigma-Aldrich, MABE343, 1:10000 dilution), anti-NP (ab20343, Abcam, 1:1000 dilution), anti-PB1 (Santa Cruz Biotechnology, 1:1000 dilution), anti-PB2 (Santa Cruz Biotechnology, 1:1000 dilution), anti-HA (clone C102, Santa Cruz Biotechnology, 1:1000 dilution), anti-M1 (FluAc, Santa Cruz Biotechnology, 1:1000 dilution), anti-M2 (clone 14C2, Santa Cruz Biotechnology, 1:1000 dilution), anti-NS1 (Santa Cruz Biotechnology, 1:1000 dilution), anti-IRE1α (clone 14C10 (3294), Cell Signaling Technology, 1:1000 dilution), anti-eIF2α (clone D-3, Santa Cruz Biotechnology, 1:1000 dilution), anti-phospho-eIF2α (clone S1, Abcam, 1:1000 dilution), anti-ATF4 (Bioworld, 1:1000 dilution), anti-phospho-ATF4 (Bioworld, 1:1000 dilution), anti-GADD34 (Invitrogen; 1:1000 dilution), anti-GAPDH (Cell signalling, 1:1000 dilution), anti-actin (Cell signalling, 1:5000 dilution), anti-vinculin (ProteinTech, 1:5000 dilution), β-tubulin anti-mouse (Life Technologies, 1:5000 dilution), and β-tubulin anti-rabbit (ProteinTech, 1:5000 dilution) overnight at 4°C. .. The secondary antibodies IRDye 680® Goat anti-rabbit or IRDye® 800 CW Goat anti-mouse (LI-COR Biosciences, 1:10000 dilution) were used for signal detection in an Odyssey Infrared Imaging system (LI-COR Biosciences).

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. Sections were then pre-treated for 30 min with 3% hydrogen peroxide (Sigma-Aldrich, 216763) in PBS, washed twice with 0.1 M tris-buffered saline (TBS), blocked for 1 h in 2% bovine serum albumin (BSA) in TBS containing 0.1% polysorbate 20 (TBS-T) and 10 min in 2.5% normal horse serum (Thermo Scientific, #R37625), and incubated with primary antibodies diluted appropriately in blocking solution. .. The following day, sections were washed in TBS-T and incubated with ready-to-use secondary antibody (Vector Laboratories, #MP-7401) for 1 h and the staining was revealed with DAB+ solution (Dako, #Κ3467).

    Incubation:

    Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications
    Article Snippet: After deparaffinized and rehydrated, antigen retrieval was performed using antigen unmasking solution (H-3300, Vector Lab), and endogenous peroxidase activity was blocked with BLOXALL Blocking Solution (SP-6000, Vector Lab) for 10 min. .. Following several washes with tris-buffered saline (TBS), tissues were blocked with 2.5% normal horse serum (NHS), incubated with primary antibody (rabbit monoclonal anti-TNF alpha, ab183218, Abcam, 1:500) and matrix metallopeptidase 9 (MMP9) (Mouse monoclonal anti-MMP9, MA5-15886, Invitrogen, 1:200) in 1% NHS for 30 min, treated with biotinylated universal antibody (BUA, anti-mouse/rabbit IgG) for 30 min, and then incubated with avidin-biotin complex (ABC) reagent for 30 min, which are contained in VETASTAIN Elite ABC-HRP kit (PK-7200, Vector Lab). .. The color development was achieved using ImmPACT® DAB Substrate Kit (SK-4105, horseradish peroxidase (HRP), Vector Lab), followed by counterstaining with Hematoxylin QS (H-3404) for 30 s. After being rinsed with DW, stained tissues were mounted with VectaMount QA Mounting Medium (H-5501, Vector Lab) and covered with a coverslip.

    Article Title: Importin α1 contributes to Venezuelan equine encephalitis virus-induced cell death, but is not required for the capsid-mediated blockage of nucleocytoplasmic trafficking
    Article Snippet: Mouse anti-V5 (BioRad, MCA1360) and rabbit anti-KPNA2 (Bethyl, A300-484A) were used at 1:10000; rabbit anti-CRM1 (Bethyl, A300-469A) was used at 1:2000; anti-KPNA3 (Bethyl, A301-626A) at 1:500, and anti-KPNA4 (Proteintech, 12463-1-AP) at 1:1000. .. Following three 5-min washes in 1x TBST and one in 1x Tris-Buffered Saline (TBS), membranes were incubated with secondary antibodies at 1:2000 (goat anti-mouse IgG, Invitrogen 32430; or goat anti-rabbit IgG, Invitrogen 32460) prepared in 0.5% milk/TBST for 1 h at room temperature. .. After identical washing steps, membranes were developed using the SuperSignalTM West Femto Maximum Sensitivity Substrate (ThermoFisher, 34095) and imaged with a BioRad ChemiDocTM Imaging system.

    Article Title: Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) platform elicits enhanced humoral and cellular immune responses
    Article Snippet: .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20. .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20.

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. Sections were then pre-treated for 30 min with 3% hydrogen peroxide (Sigma-Aldrich, 216763) in PBS, washed twice with 0.1 M tris-buffered saline (TBS), blocked for 1 h in 2% bovine serum albumin (BSA) in TBS containing 0.1% polysorbate 20 (TBS-T) and 10 min in 2.5% normal horse serum (Thermo Scientific, #R37625), and incubated with primary antibodies diluted appropriately in blocking solution. .. The following day, sections were washed in TBS-T and incubated with ready-to-use secondary antibody (Vector Laboratories, #MP-7401) for 1 h and the staining was revealed with DAB+ solution (Dako, #Κ3467).

    Avidin-Biotin Assay:

    Article Title: Thermoreversible cell-derived extracellular matrix only hydrogel (CEOgel): Development, characterization, and applications
    Article Snippet: After deparaffinized and rehydrated, antigen retrieval was performed using antigen unmasking solution (H-3300, Vector Lab), and endogenous peroxidase activity was blocked with BLOXALL Blocking Solution (SP-6000, Vector Lab) for 10 min. .. Following several washes with tris-buffered saline (TBS), tissues were blocked with 2.5% normal horse serum (NHS), incubated with primary antibody (rabbit monoclonal anti-TNF alpha, ab183218, Abcam, 1:500) and matrix metallopeptidase 9 (MMP9) (Mouse monoclonal anti-MMP9, MA5-15886, Invitrogen, 1:200) in 1% NHS for 30 min, treated with biotinylated universal antibody (BUA, anti-mouse/rabbit IgG) for 30 min, and then incubated with avidin-biotin complex (ABC) reagent for 30 min, which are contained in VETASTAIN Elite ABC-HRP kit (PK-7200, Vector Lab). .. The color development was achieved using ImmPACT® DAB Substrate Kit (SK-4105, horseradish peroxidase (HRP), Vector Lab), followed by counterstaining with Hematoxylin QS (H-3404) for 30 s. After being rinsed with DW, stained tissues were mounted with VectaMount QA Mounting Medium (H-5501, Vector Lab) and covered with a coverslip.

    Blocking Assay:

    Article Title: Subunit vaccination using Atomic Layering Thermostable Antigen and Adjuvant (ALTA ® ) platform elicits enhanced humoral and cellular immune responses
    Article Snippet: .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20. .. The plates were blocked at room temperature for 30 min with StartingBlock T20 [Tris-buffered saline (TBS)] blocking buffer (Thermo Fisher Scientific) and then incubated for 1 h at 37 °C with mouse serum samples serially diluted in TBS plus 0.05% Tween 20 (TBST; pH 7.5) plus 10% StartingBlock T20.

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. Sections were then pre-treated for 30 min with 3% hydrogen peroxide (Sigma-Aldrich, 216763) in PBS, washed twice with 0.1 M tris-buffered saline (TBS), blocked for 1 h in 2% bovine serum albumin (BSA) in TBS containing 0.1% polysorbate 20 (TBS-T) and 10 min in 2.5% normal horse serum (Thermo Scientific, #R37625), and incubated with primary antibodies diluted appropriately in blocking solution. .. The following day, sections were washed in TBS-T and incubated with ready-to-use secondary antibody (Vector Laboratories, #MP-7401) for 1 h and the staining was revealed with DAB+ solution (Dako, #Κ3467).

    Recombinant:

    Article Title: Peri-Microvascular Glycogen and Lactate Regulate Capillary Constrictions and Ischemia Outcome in Mice.
    Article Snippet: Reagent or Resource Source Identifier Antibodies Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 488 Vector Labs Cat. No:Cat. No: DL1174- 1, RRID: N/A Aminopeptidase N/ CD13 Antibody Acris Antibodies GmBH Cat. No:Cat. No: AM26636AF- N, RRID: N/A NG2 Millipore Cat. No:Cat. No: AB5320, RRID: AB_91789 PDGFR- β Abcam Cat. No:Cat. No: ab69506, RRID: AB_1269704 GYS- 1 Cell Signaling Technologies Cat. No:Cat. No: 3893, RRID: AB_2279563 Beta- Actin ThermoFisher Scientific Cat. No: PA1- 183, RRID: AB_2608409 GFAP Abcam Cat. No: ab4674, RRID: AB_304558 MCT1 Polyclonal antibody Proteintech Cat. No: 20139- 1- AP, RRID: AB_2878645 MCT12 Polyclonal antibody Proteintech Cat. No: 20553- 1- AP, RRID: AB_10693621 Hexokinase 2 Polyclonal antibody Proteintech Cat. No: 22029- 1- AP, RRID: AB_11182717 GPR81 Antibody Novus Biologicals Cat. No: NLS2095, RRID: AB_10001932 IV58B6 courtesy of Dr. Otto Baba N/A ESG1A9 courtesy of Dr. Hitoshi Ashida N/A AffiniPure F(ab’)2 Fragment Donkey AntiMouse IgM Jackson Immunoresearch Cat. No: 715- 006- 020, RRID: AB_2340760 Alexa Fluor 488 AffiniPure Donkey Anti- Mouse IgM Jackson Immunoresearch Cat. No: 715- 545- 140, RRID: AB_2340845 Alexa Fluor 488 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11008, RRID: N/A Alexa Fluor 594 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11012, RRID: N/A Alexa Fluor 647 AffiniPure Donkey Anti- Chicken IgY (IgG) (H + L) Jackson Immunoresearch Cat. No: 703- 605- 155, RRID: AB_2340379 Alexa Fluor 568 AffiniPure Donkey Anti- Rat IgG (H + L) Jackson Immunoresearch Cat. No: 712- 575- 153, RRID: AB_3095476 14714159, 2026, 4, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jnc.70430, W iley O nline L ibrary on [17/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 7 of 27Journal of Neurochemistry, 2026 Reagent or Resource Source Identifier Goat anti- Mouse IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21040, RRID: AB_2536527 Goat anti- Rabbit IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21234, RRID: AB_1500696 Chemicals, peptides, and recombinant proteins DAB (1,4- dideoksi1,4- imino- d- arabinitol hydrochloride) Sigma- Aldrich Cat. No: D1542 Sodium D- lactate Sigma- Aldrich Cat. No: 71716 Sodium L- lactate Sigma- Aldrich Cat. No: 71718 Chloral hydrate Sigma- Aldrich Cat. No: 05–2650 Periodic acid Sigma- Aldrich Cat. No: 375810 Schiff 's reagent Merck Cat. No: 3952016 Dimedone (5,5- Dimethyl- 1,3- cyclohexanedione) Sigma- Aldrich Cat. No: D153303 TRIS- buffered saline (TBS, 10X) pH 7.4 ThermoFisher Cat. No: J60764.K2 RIPA Lysis and Extraction Buffer Sigma- Aldrich Cat. No: 89900 Triton- X 100 Sigma- Aldrich Cat. No: X100 Tween- 20 Sigma- Aldrich Cat. No: P1379 Halt Protease and Phosphatase Inhibitor Cocktail (100X) ThermoFisher Cat. No: 78440 NuPAGE Bis- Tris Mini Protein Gels, 4%–12%, 1.0–1.5 mm ThermoFisher Cat. No: NP0322BOX NuPAGE MOPS SDS Running Buffer (20X) ThermoFisher Cat. No: NP0001 PVDF Transfer Membranes ThermoFisher Cat. No: 88518 VECTASHIELD PLUS Antifade Mounting Medium with DAPI (H- 2000) Vector Labs Cat. No: H- 2000, RRID: N/A Experimental models: Organisms/strains Swiss Albino mice Jackson Laboratories Strain #:034608 RRID:IMSR_ JAX:034608 C57Bl/6 wild- type (WT) mice Jackson Laboratories Strain: #:000664 RRID: IMSR_JAX:000664 GYS1Nestin- KO mice Courtesy of Dr. Duran and Dr. Guinovart N/A GYS1Gfap- KO mice Reagent or Resource Source Identifier Software and algorithms ImageJ/Fiji NIH https:// imagej. net/ softw are/ fiji/ Prism- GraphPad Dotmatics https:// www. graph pad. com/ scien tific- softw are/ prism/ www. graph pad. com/ scien tificsoftw are/ prism/ Endnote 20 Clarivate https:// endno te. com/? langu age= en Adobe Photoshop Adobe https:// www. adobe. com/ produ cts/ photo shop. html/ Adobe Illustrator Adobe https:// www. adobe. com/ produ cts/ illus trator. html/ ..

    Lysis:

    Article Title: Peri-Microvascular Glycogen and Lactate Regulate Capillary Constrictions and Ischemia Outcome in Mice.
    Article Snippet: Reagent or Resource Source Identifier Antibodies Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 488 Vector Labs Cat. No:Cat. No: DL1174- 1, RRID: N/A Aminopeptidase N/ CD13 Antibody Acris Antibodies GmBH Cat. No:Cat. No: AM26636AF- N, RRID: N/A NG2 Millipore Cat. No:Cat. No: AB5320, RRID: AB_91789 PDGFR- β Abcam Cat. No:Cat. No: ab69506, RRID: AB_1269704 GYS- 1 Cell Signaling Technologies Cat. No:Cat. No: 3893, RRID: AB_2279563 Beta- Actin ThermoFisher Scientific Cat. No: PA1- 183, RRID: AB_2608409 GFAP Abcam Cat. No: ab4674, RRID: AB_304558 MCT1 Polyclonal antibody Proteintech Cat. No: 20139- 1- AP, RRID: AB_2878645 MCT12 Polyclonal antibody Proteintech Cat. No: 20553- 1- AP, RRID: AB_10693621 Hexokinase 2 Polyclonal antibody Proteintech Cat. No: 22029- 1- AP, RRID: AB_11182717 GPR81 Antibody Novus Biologicals Cat. No: NLS2095, RRID: AB_10001932 IV58B6 courtesy of Dr. Otto Baba N/A ESG1A9 courtesy of Dr. Hitoshi Ashida N/A AffiniPure F(ab’)2 Fragment Donkey AntiMouse IgM Jackson Immunoresearch Cat. No: 715- 006- 020, RRID: AB_2340760 Alexa Fluor 488 AffiniPure Donkey Anti- Mouse IgM Jackson Immunoresearch Cat. No: 715- 545- 140, RRID: AB_2340845 Alexa Fluor 488 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11008, RRID: N/A Alexa Fluor 594 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11012, RRID: N/A Alexa Fluor 647 AffiniPure Donkey Anti- Chicken IgY (IgG) (H + L) Jackson Immunoresearch Cat. No: 703- 605- 155, RRID: AB_2340379 Alexa Fluor 568 AffiniPure Donkey Anti- Rat IgG (H + L) Jackson Immunoresearch Cat. No: 712- 575- 153, RRID: AB_3095476 14714159, 2026, 4, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jnc.70430, W iley O nline L ibrary on [17/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 7 of 27Journal of Neurochemistry, 2026 Reagent or Resource Source Identifier Goat anti- Mouse IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21040, RRID: AB_2536527 Goat anti- Rabbit IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21234, RRID: AB_1500696 Chemicals, peptides, and recombinant proteins DAB (1,4- dideoksi1,4- imino- d- arabinitol hydrochloride) Sigma- Aldrich Cat. No: D1542 Sodium D- lactate Sigma- Aldrich Cat. No: 71716 Sodium L- lactate Sigma- Aldrich Cat. No: 71718 Chloral hydrate Sigma- Aldrich Cat. No: 05–2650 Periodic acid Sigma- Aldrich Cat. No: 375810 Schiff 's reagent Merck Cat. No: 3952016 Dimedone (5,5- Dimethyl- 1,3- cyclohexanedione) Sigma- Aldrich Cat. No: D153303 TRIS- buffered saline (TBS, 10X) pH 7.4 ThermoFisher Cat. No: J60764.K2 RIPA Lysis and Extraction Buffer Sigma- Aldrich Cat. No: 89900 Triton- X 100 Sigma- Aldrich Cat. No: X100 Tween- 20 Sigma- Aldrich Cat. No: P1379 Halt Protease and Phosphatase Inhibitor Cocktail (100X) ThermoFisher Cat. No: 78440 NuPAGE Bis- Tris Mini Protein Gels, 4%–12%, 1.0–1.5 mm ThermoFisher Cat. No: NP0322BOX NuPAGE MOPS SDS Running Buffer (20X) ThermoFisher Cat. No: NP0001 PVDF Transfer Membranes ThermoFisher Cat. No: 88518 VECTASHIELD PLUS Antifade Mounting Medium with DAPI (H- 2000) Vector Labs Cat. No: H- 2000, RRID: N/A Experimental models: Organisms/strains Swiss Albino mice Jackson Laboratories Strain #:034608 RRID:IMSR_ JAX:034608 C57Bl/6 wild- type (WT) mice Jackson Laboratories Strain: #:000664 RRID: IMSR_JAX:000664 GYS1Nestin- KO mice Courtesy of Dr. Duran and Dr. Guinovart N/A GYS1Gfap- KO mice Reagent or Resource Source Identifier Software and algorithms ImageJ/Fiji NIH https:// imagej. net/ softw are/ fiji/ Prism- GraphPad Dotmatics https:// www. graph pad. com/ scien tific- softw are/ prism/ www. graph pad. com/ scien tificsoftw are/ prism/ Endnote 20 Clarivate https:// endno te. com/? langu age= en Adobe Photoshop Adobe https:// www. adobe. com/ produ cts/ photo shop. html/ Adobe Illustrator Adobe https:// www. adobe. com/ produ cts/ illus trator. html/ ..

    Extraction:

    Article Title: Peri-Microvascular Glycogen and Lactate Regulate Capillary Constrictions and Ischemia Outcome in Mice.
    Article Snippet: Reagent or Resource Source Identifier Antibodies Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 488 Vector Labs Cat. No:Cat. No: DL1174- 1, RRID: N/A Aminopeptidase N/ CD13 Antibody Acris Antibodies GmBH Cat. No:Cat. No: AM26636AF- N, RRID: N/A NG2 Millipore Cat. No:Cat. No: AB5320, RRID: AB_91789 PDGFR- β Abcam Cat. No:Cat. No: ab69506, RRID: AB_1269704 GYS- 1 Cell Signaling Technologies Cat. No:Cat. No: 3893, RRID: AB_2279563 Beta- Actin ThermoFisher Scientific Cat. No: PA1- 183, RRID: AB_2608409 GFAP Abcam Cat. No: ab4674, RRID: AB_304558 MCT1 Polyclonal antibody Proteintech Cat. No: 20139- 1- AP, RRID: AB_2878645 MCT12 Polyclonal antibody Proteintech Cat. No: 20553- 1- AP, RRID: AB_10693621 Hexokinase 2 Polyclonal antibody Proteintech Cat. No: 22029- 1- AP, RRID: AB_11182717 GPR81 Antibody Novus Biologicals Cat. No: NLS2095, RRID: AB_10001932 IV58B6 courtesy of Dr. Otto Baba N/A ESG1A9 courtesy of Dr. Hitoshi Ashida N/A AffiniPure F(ab’)2 Fragment Donkey AntiMouse IgM Jackson Immunoresearch Cat. No: 715- 006- 020, RRID: AB_2340760 Alexa Fluor 488 AffiniPure Donkey Anti- Mouse IgM Jackson Immunoresearch Cat. No: 715- 545- 140, RRID: AB_2340845 Alexa Fluor 488 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11008, RRID: N/A Alexa Fluor 594 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11012, RRID: N/A Alexa Fluor 647 AffiniPure Donkey Anti- Chicken IgY (IgG) (H + L) Jackson Immunoresearch Cat. No: 703- 605- 155, RRID: AB_2340379 Alexa Fluor 568 AffiniPure Donkey Anti- Rat IgG (H + L) Jackson Immunoresearch Cat. No: 712- 575- 153, RRID: AB_3095476 14714159, 2026, 4, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jnc.70430, W iley O nline L ibrary on [17/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 7 of 27Journal of Neurochemistry, 2026 Reagent or Resource Source Identifier Goat anti- Mouse IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21040, RRID: AB_2536527 Goat anti- Rabbit IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21234, RRID: AB_1500696 Chemicals, peptides, and recombinant proteins DAB (1,4- dideoksi1,4- imino- d- arabinitol hydrochloride) Sigma- Aldrich Cat. No: D1542 Sodium D- lactate Sigma- Aldrich Cat. No: 71716 Sodium L- lactate Sigma- Aldrich Cat. No: 71718 Chloral hydrate Sigma- Aldrich Cat. No: 05–2650 Periodic acid Sigma- Aldrich Cat. No: 375810 Schiff 's reagent Merck Cat. No: 3952016 Dimedone (5,5- Dimethyl- 1,3- cyclohexanedione) Sigma- Aldrich Cat. No: D153303 TRIS- buffered saline (TBS, 10X) pH 7.4 ThermoFisher Cat. No: J60764.K2 RIPA Lysis and Extraction Buffer Sigma- Aldrich Cat. No: 89900 Triton- X 100 Sigma- Aldrich Cat. No: X100 Tween- 20 Sigma- Aldrich Cat. No: P1379 Halt Protease and Phosphatase Inhibitor Cocktail (100X) ThermoFisher Cat. No: 78440 NuPAGE Bis- Tris Mini Protein Gels, 4%–12%, 1.0–1.5 mm ThermoFisher Cat. No: NP0322BOX NuPAGE MOPS SDS Running Buffer (20X) ThermoFisher Cat. No: NP0001 PVDF Transfer Membranes ThermoFisher Cat. No: 88518 VECTASHIELD PLUS Antifade Mounting Medium with DAPI (H- 2000) Vector Labs Cat. No: H- 2000, RRID: N/A Experimental models: Organisms/strains Swiss Albino mice Jackson Laboratories Strain #:034608 RRID:IMSR_ JAX:034608 C57Bl/6 wild- type (WT) mice Jackson Laboratories Strain: #:000664 RRID: IMSR_JAX:000664 GYS1Nestin- KO mice Courtesy of Dr. Duran and Dr. Guinovart N/A GYS1Gfap- KO mice Reagent or Resource Source Identifier Software and algorithms ImageJ/Fiji NIH https:// imagej. net/ softw are/ fiji/ Prism- GraphPad Dotmatics https:// www. graph pad. com/ scien tific- softw are/ prism/ www. graph pad. com/ scien tificsoftw are/ prism/ Endnote 20 Clarivate https:// endno te. com/? langu age= en Adobe Photoshop Adobe https:// www. adobe. com/ produ cts/ photo shop. html/ Adobe Illustrator Adobe https:// www. adobe. com/ produ cts/ illus trator. html/ ..

    Software:

    Article Title: Peri-Microvascular Glycogen and Lactate Regulate Capillary Constrictions and Ischemia Outcome in Mice.
    Article Snippet: Reagent or Resource Source Identifier Antibodies Lycopersicon esculentum (Tomato) Lectin (LEL, TL), DyLight 488 Vector Labs Cat. No:Cat. No: DL1174- 1, RRID: N/A Aminopeptidase N/ CD13 Antibody Acris Antibodies GmBH Cat. No:Cat. No: AM26636AF- N, RRID: N/A NG2 Millipore Cat. No:Cat. No: AB5320, RRID: AB_91789 PDGFR- β Abcam Cat. No:Cat. No: ab69506, RRID: AB_1269704 GYS- 1 Cell Signaling Technologies Cat. No:Cat. No: 3893, RRID: AB_2279563 Beta- Actin ThermoFisher Scientific Cat. No: PA1- 183, RRID: AB_2608409 GFAP Abcam Cat. No: ab4674, RRID: AB_304558 MCT1 Polyclonal antibody Proteintech Cat. No: 20139- 1- AP, RRID: AB_2878645 MCT12 Polyclonal antibody Proteintech Cat. No: 20553- 1- AP, RRID: AB_10693621 Hexokinase 2 Polyclonal antibody Proteintech Cat. No: 22029- 1- AP, RRID: AB_11182717 GPR81 Antibody Novus Biologicals Cat. No: NLS2095, RRID: AB_10001932 IV58B6 courtesy of Dr. Otto Baba N/A ESG1A9 courtesy of Dr. Hitoshi Ashida N/A AffiniPure F(ab’)2 Fragment Donkey AntiMouse IgM Jackson Immunoresearch Cat. No: 715- 006- 020, RRID: AB_2340760 Alexa Fluor 488 AffiniPure Donkey Anti- Mouse IgM Jackson Immunoresearch Cat. No: 715- 545- 140, RRID: AB_2340845 Alexa Fluor 488 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11008, RRID: N/A Alexa Fluor 594 conjugated Goat antiRabbit IgG (H&L) ThermoFisher Scientific Cat. No: A- 11012, RRID: N/A Alexa Fluor 647 AffiniPure Donkey Anti- Chicken IgY (IgG) (H + L) Jackson Immunoresearch Cat. No: 703- 605- 155, RRID: AB_2340379 Alexa Fluor 568 AffiniPure Donkey Anti- Rat IgG (H + L) Jackson Immunoresearch Cat. No: 712- 575- 153, RRID: AB_3095476 14714159, 2026, 4, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jnc.70430, W iley O nline L ibrary on [17/04/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 7 of 27Journal of Neurochemistry, 2026 Reagent or Resource Source Identifier Goat anti- Mouse IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21040, RRID: AB_2536527 Goat anti- Rabbit IgG (H + L) Cross- Adsorbed Secondary Antibody, HRP ThermoFisher Scientific Cat. No: G- 21234, RRID: AB_1500696 Chemicals, peptides, and recombinant proteins DAB (1,4- dideoksi1,4- imino- d- arabinitol hydrochloride) Sigma- Aldrich Cat. No: D1542 Sodium D- lactate Sigma- Aldrich Cat. No: 71716 Sodium L- lactate Sigma- Aldrich Cat. No: 71718 Chloral hydrate Sigma- Aldrich Cat. No: 05–2650 Periodic acid Sigma- Aldrich Cat. No: 375810 Schiff 's reagent Merck Cat. No: 3952016 Dimedone (5,5- Dimethyl- 1,3- cyclohexanedione) Sigma- Aldrich Cat. No: D153303 TRIS- buffered saline (TBS, 10X) pH 7.4 ThermoFisher Cat. No: J60764.K2 RIPA Lysis and Extraction Buffer Sigma- Aldrich Cat. No: 89900 Triton- X 100 Sigma- Aldrich Cat. No: X100 Tween- 20 Sigma- Aldrich Cat. No: P1379 Halt Protease and Phosphatase Inhibitor Cocktail (100X) ThermoFisher Cat. No: 78440 NuPAGE Bis- Tris Mini Protein Gels, 4%–12%, 1.0–1.5 mm ThermoFisher Cat. No: NP0322BOX NuPAGE MOPS SDS Running Buffer (20X) ThermoFisher Cat. No: NP0001 PVDF Transfer Membranes ThermoFisher Cat. No: 88518 VECTASHIELD PLUS Antifade Mounting Medium with DAPI (H- 2000) Vector Labs Cat. No: H- 2000, RRID: N/A Experimental models: Organisms/strains Swiss Albino mice Jackson Laboratories Strain #:034608 RRID:IMSR_ JAX:034608 C57Bl/6 wild- type (WT) mice Jackson Laboratories Strain: #:000664 RRID: IMSR_JAX:000664 GYS1Nestin- KO mice Courtesy of Dr. Duran and Dr. Guinovart N/A GYS1Gfap- KO mice Reagent or Resource Source Identifier Software and algorithms ImageJ/Fiji NIH https:// imagej. net/ softw are/ fiji/ Prism- GraphPad Dotmatics https:// www. graph pad. com/ scien tific- softw are/ prism/ www. graph pad. com/ scien tificsoftw are/ prism/ Endnote 20 Clarivate https:// endno te. com/? langu age= en Adobe Photoshop Adobe https:// www. adobe. com/ produ cts/ photo shop. html/ Adobe Illustrator Adobe https:// www. adobe. com/ produ cts/ illus trator. html/ ..



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