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squamous cell line ect1 e6e7  (ATCC)


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    ATCC squamous cell line ect1 e6e7
    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth <t>of</t> <t>Ect1/E6E7</t> cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)
    Squamous Cell Line Ect1 E6e7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ect1/Ect1%2FE6E7/pmc13085617-104-4-12
    Average 96 stars, based on 353 article reviews
    squamous cell line ect1 e6e7 - by Bioz Stars, 2026-11
    96/100 stars

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    1) Product Images from "Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions"

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-026-07982-w

    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)
    Figure Legend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Techniques Used: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    Related Articles

    Transmission Electron Microscopy:

    Article Title: Bacterial Extracellular Vesicles: New Hype or Hope to Explain Reproductive Host–Microbiota Interactions
    Article Snippet: 2023 , Lara et al. , Porphyromonas gingivalis , ATCC , Early stationary phase , Ultracentrifugation , DNA/RNA quantification, TEM, DLS , Yes , HTR‐8/SVneo, EA.hy926, THP‐1 (ATCC) , ‐ Porphyromonas gingivalis EVs were internalised by trophoblast cells, where they reduced oxidative stress responses and cell marker expression ‐ Conditioned media from BEV‐treated trophoblasts induced neutrophil recruitment and activation ‐ Conditioned media reduced endothelial cell migration, and promoted monocyte‐endothelial adhesion. .. 2024 , Artuyants et al. , Lactobacillus gasseri , Gardnerella vaginalis , ATCC , ON culture diluted to OD 600 = 0.05 , Ultracentrifugation, DGC, SEC , TEM, NTA, SWATH‐MS proteomics , Yes , Ect1/E6E7 (ATCC) Trichomonas vaginalis (B7RC2) , Lactobacillus gasseri EVs inhibited adhesion and growth of Trichomonas vaginalis , while Gardnerella vaginalis EVs contributed to its pathogenicity. .. 2024 , Joseph et al. , Gardnerella vaginalis, Mobiluncus mulieris, Lactobacillus crispatus , ATCC , Not mentioned, ON culture , Ultracentrifugation , TEM, ZetaView/NTA, MS , Yes , Ect1/E6E7 (ATCC), End1/E6E7 (ATCC), VK2/E6E7 (ATCC), THP‐1, TLR2 (NF‐κB‐SEAP/KI‐IL‐8 Lucia) (InvivoGen) , ‐ EVs from Gardnerella vaginalis and Mobiluncus mulieris were internalised in cervical and vaginal epithelial cells, inducing a multi‐cytokine response via TLR2 pathways ‐ EVs from Lactobacillus crispatus were internalised but did not induce inflammatory responses.

    Data-independent acquisition:

    Article Title: Bacterial Extracellular Vesicles: New Hype or Hope to Explain Reproductive Host–Microbiota Interactions
    Article Snippet: 2023 , Lara et al. , Porphyromonas gingivalis , ATCC , Early stationary phase , Ultracentrifugation , DNA/RNA quantification, TEM, DLS , Yes , HTR‐8/SVneo, EA.hy926, THP‐1 (ATCC) , ‐ Porphyromonas gingivalis EVs were internalised by trophoblast cells, where they reduced oxidative stress responses and cell marker expression ‐ Conditioned media from BEV‐treated trophoblasts induced neutrophil recruitment and activation ‐ Conditioned media reduced endothelial cell migration, and promoted monocyte‐endothelial adhesion. .. 2024 , Artuyants et al. , Lactobacillus gasseri , Gardnerella vaginalis , ATCC , ON culture diluted to OD 600 = 0.05 , Ultracentrifugation, DGC, SEC , TEM, NTA, SWATH‐MS proteomics , Yes , Ect1/E6E7 (ATCC) Trichomonas vaginalis (B7RC2) , Lactobacillus gasseri EVs inhibited adhesion and growth of Trichomonas vaginalis , while Gardnerella vaginalis EVs contributed to its pathogenicity. .. 2024 , Joseph et al. , Gardnerella vaginalis, Mobiluncus mulieris, Lactobacillus crispatus , ATCC , Not mentioned, ON culture , Ultracentrifugation , TEM, ZetaView/NTA, MS , Yes , Ect1/E6E7 (ATCC), End1/E6E7 (ATCC), VK2/E6E7 (ATCC), THP‐1, TLR2 (NF‐κB‐SEAP/KI‐IL‐8 Lucia) (InvivoGen) , ‐ EVs from Gardnerella vaginalis and Mobiluncus mulieris were internalised in cervical and vaginal epithelial cells, inducing a multi‐cytokine response via TLR2 pathways ‐ EVs from Lactobacillus crispatus were internalised but did not induce inflammatory responses.

    Cell Culture:

    Article Title: Monkeypox virus replication and host response in vaginal and ectocervical epithelial cells
    Article Snippet: .. Human Vaginal Epithelial Cells VK2/E6E7 and Human Ectocervical Epithelial Cells Ect1/E6E7 (CRL-2616 and CRL-2614 obtained from ATCC, Manassas, Virginia) were cultured in Keratinocyte Serum-Free Medium (KSFM), containing 0.1 ng/mL Human Recombinant Epithelial Growth Factor (EGF) and 0.05 mg/mL Bovine Pituitary Extract (BPE) (Thermo Fisher Scientific, Waltham, Massachusetts). .. Vero E6 cells (CRL-1586 obtained from ATCC, Manassas, Virginia) were cultured in Eagle’s Minimum Essential medium (E-MEM) containing L-glutamine (Sigma-Aldrich, St. Louis, Missouri) and 10% Foetal Bovine serum (Corning, New York).

    Article Title: Exposure to Antibiotics Modifies the Immune Profiles of Bacterial Extracellular Vesicles from Common Vaginal Anaerobes
    Article Snippet: .. Human ectocervical (Ect; American Type Culture Collection (ATCC) CRL-2614, Ect1/E6E7, Manassas, VA, USA), endocervical (End; ATCC CRL-2615, End1/E6E7), and vaginal (VK2; ATCC CRL-2616, VK2/E6E7) epithelial cell lines were cultured in keratinocyte-serum-free medium supplemented with 0.1 ng/mL epidermal growth factor, 50 μg/mL bovine pituitary extract (Gibco/Thermo Fisher Scientific, Waltham, MA, USA), and 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco). .. THP-1-Null cells (THP1) and THP1-Dual KO-TLR2 cells (THP1-TLR2KO; InvivoGen, San Diego, CA, USA) were cultured in RPMI-1640 medium (Life Technologies, Grand Island, NY, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (HI-FBS) (Gibco) and 100 U/mL penicillin and 100 μg/mL streptomycin.

    Article Title: Monkeypox virus replication and host response in vaginal and ectocervical epithelial cells.
    Article Snippet: .. Human Vaginal Epithelial Cells VK2/E6E7 and Human Ectocervical Epithelial Cells Ect1/E6E7 (CRL-2616 and CRL-2614 obtained from ATCC, Manassas, Virginia) were cultured in Keratinocyte Serum-Free Medium (KSFM), containing 0.1 ng/mL Human Recombinant Epithelial Growth Factor (EGF) and 0.05 mg/mL Bovine Pituitary Extract (BPE) (Thermo Fisher Scientific, Waltham, Massachusetts). .. Vero E6 cells (CRL-1586 obtained from ATCC, Manassas, Virginia) were cultured in Eagle’s Minimum Essential medium (E-MEM) containing L-glutamine (Sigma-Aldrich, St. Louis, Missouri) and 10% Foetal Bovine serum (Corning, New York).

    Article Title: Multifunctional microfluidic device for capturing target cells and analyzing genomic DNA isolated from the target cells while under flow conditions
    Article Snippet: .. Cell Culture and Buffers: HeLa, CAOV-3, Ect1/E6E7, and End1/E6E7 cells were purchased from American Type Cell Culture (ATCC). .. The HeLa and CAOV-3 cells were cultured in DMEM media (Life Technologies) supplemented with non-essential amino acids, 110 mg/L sodium pyruvate, 200 mM L-glutamine (Life Technologies), 1× Pen Strep (Life Technologies), 26.8 mM HEPES, betamercaptoethanol, and containing 10% fetal bovine serum.

    Article Title: Trichomonas vaginalis-shaped inflammatory microenvironment drives macrophage immune signaling and modulates cervical cancer cell proliferation and migration
    Article Snippet: The human monocytic cell line THP-1 (ATCC TIB-202) was grown in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and incubated in 5% CO2 at 37°C. .. The human ectocervical epithelial cell line Ect1/E6E7 (ATCC CRL-2614TM) was cultured in keratinocyte serum-free medium (KSFM) (Gibco, Thermo Fisher Scientific, Inc.,Waltham, MA, USA) supplemented with 0.1 ng/ml recombinant human epidermal growth factor (EGF), 0.05 mg/ml bovine pituitary extract, and 0.4 mM calcium chloride, and incubated in 5% CO2 at 37°C. ..

    Recombinant:

    Article Title: Monkeypox virus replication and host response in vaginal and ectocervical epithelial cells
    Article Snippet: .. Human Vaginal Epithelial Cells VK2/E6E7 and Human Ectocervical Epithelial Cells Ect1/E6E7 (CRL-2616 and CRL-2614 obtained from ATCC, Manassas, Virginia) were cultured in Keratinocyte Serum-Free Medium (KSFM), containing 0.1 ng/mL Human Recombinant Epithelial Growth Factor (EGF) and 0.05 mg/mL Bovine Pituitary Extract (BPE) (Thermo Fisher Scientific, Waltham, Massachusetts). .. Vero E6 cells (CRL-1586 obtained from ATCC, Manassas, Virginia) were cultured in Eagle’s Minimum Essential medium (E-MEM) containing L-glutamine (Sigma-Aldrich, St. Louis, Missouri) and 10% Foetal Bovine serum (Corning, New York).

    Article Title: Monkeypox virus replication and host response in vaginal and ectocervical epithelial cells.
    Article Snippet: .. Human Vaginal Epithelial Cells VK2/E6E7 and Human Ectocervical Epithelial Cells Ect1/E6E7 (CRL-2616 and CRL-2614 obtained from ATCC, Manassas, Virginia) were cultured in Keratinocyte Serum-Free Medium (KSFM), containing 0.1 ng/mL Human Recombinant Epithelial Growth Factor (EGF) and 0.05 mg/mL Bovine Pituitary Extract (BPE) (Thermo Fisher Scientific, Waltham, Massachusetts). .. Vero E6 cells (CRL-1586 obtained from ATCC, Manassas, Virginia) were cultured in Eagle’s Minimum Essential medium (E-MEM) containing L-glutamine (Sigma-Aldrich, St. Louis, Missouri) and 10% Foetal Bovine serum (Corning, New York).

    Article Title: Trichomonas vaginalis-shaped inflammatory microenvironment drives macrophage immune signaling and modulates cervical cancer cell proliferation and migration
    Article Snippet: The human monocytic cell line THP-1 (ATCC TIB-202) was grown in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and incubated in 5% CO2 at 37°C. .. The human ectocervical epithelial cell line Ect1/E6E7 (ATCC CRL-2614TM) was cultured in keratinocyte serum-free medium (KSFM) (Gibco, Thermo Fisher Scientific, Inc.,Waltham, MA, USA) supplemented with 0.1 ng/ml recombinant human epidermal growth factor (EGF), 0.05 mg/ml bovine pituitary extract, and 0.4 mM calcium chloride, and incubated in 5% CO2 at 37°C. ..

    Incubation:

    Article Title: Trichomonas vaginalis-shaped inflammatory microenvironment drives macrophage immune signaling and modulates cervical cancer cell proliferation and migration
    Article Snippet: The human monocytic cell line THP-1 (ATCC TIB-202) was grown in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and incubated in 5% CO2 at 37°C. .. The human ectocervical epithelial cell line Ect1/E6E7 (ATCC CRL-2614TM) was cultured in keratinocyte serum-free medium (KSFM) (Gibco, Thermo Fisher Scientific, Inc.,Waltham, MA, USA) supplemented with 0.1 ng/ml recombinant human epidermal growth factor (EGF), 0.05 mg/ml bovine pituitary extract, and 0.4 mM calcium chloride, and incubated in 5% CO2 at 37°C. ..



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    Image Search Results


    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    doi: 10.3389/fcell.2026.1773562

    Figure Lengend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Article Snippet: HeLa and Ect1 cell lines were obtained from ATCC.

    Techniques: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay