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human ectocervical epithelial cell line ect1 e6e7  (ATCC)


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    ATCC human ectocervical epithelial cell line ect1 e6e7
    Human Ectocervical Epithelial Cell Line Ect1 E6e7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ect1/Ect1%2FE6E7/10__1186_slash_s13071___026___07616___6-65-1-7
    Average 96 stars, based on 352 article reviews
    human ectocervical epithelial cell line ect1 e6e7 - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Spatiotemporal regulation of human IFN-ε and innate immunity in the female reproductive tract.
    Article Snippet: .. VK2 (vaginal; ATCC CRL-2616), Ect1 (ectocervical; ATCC CRL-2614), and End1 (endocervical; ATCC CRL-2615) cell lines were maintained in keratinocyte-SFM (Thermo Fisher Scientific), supplemented with 0.2 ng/mL human recombinant EGF, 20 μg/mL recombinant bovine pituitary extract, and 1 2 R E S E A R C H A R T I C L E JCI Insight 2022;7(18):e135407 https://doi.org/10.1172/jci.insight.135407 1% Antibiotic-Antimycotic (Gibco, Thermo Fisher Scientific). .. ECC-1 endometrial epithelial cells (ATCC) were cultured in DMEM:F12 Glutamax (Gibco, Thermo Fisher Scientific) supplemented with 1% penicillin/streptomycin and 10% vol/vol FBS, and validity was routinely tested using short tandem repeat DNA profiling of human cell lines per ATCC guidelines.

    Article Title: Spatiotemporal regulation of human IFN- ε and innate immunity in the female reproductive tract
    Article Snippet: .. VK2 (vaginal; ATCC CRL-2616), Ect1 (ectocervical; ATCC CRL-2614), and End1 (endocervical; ATCC CRL-2615) cell lines were maintained in keratinocyte-SFM (Thermo Fisher Scientific), supplemented with 0.2 ng/mL human recombinant EGF, 20 μg/mL recombinant bovine pituitary extract, and 1% Antibiotic-Antimycotic (Gibco, Thermo Fisher Scientific). .. ECC-1 endometrial epithelial cells (ATCC) were cultured in DMEM:F12 Glutamax (Gibco, Thermo Fisher Scientific) supplemented with 1% penicillin/streptomycin and 10% vol/vol FBS, and validity was routinely tested using short tandem repeat DNA profiling of human cell lines per ATCC guidelines.

    Cell Culture:

    Article Title: miR-99a-5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD
    Article Snippet: .. The Ect1 (cat. no. CRL-2614), HeLa (cat. no. CRM-CCL-2), C33A (cat. no. HTB-31) and SiHa (cat. no. HTB-35) cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM containing 10% FBS, incubated with 5% CO 2 and 37°C. miR-99a-5p mimic and miR-NC mimic were purchased from Shanghai GenePharma Co., Ltd. Transfection of pcDNA3.1-RRAGD plasmid (30 μg), pcDNA3.1 plasmid (30 μg), miR-99a-5p mimic (100 nM; 5′-AACCCGUAGAUCCGAUCUUGUG-3′) and miR-NC mimic (100 nM; 5′-UUGUACUACACAAAAGUACUG-3′) was performed at 37°C for 48 h using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). ..

    Incubation:

    Article Title: miR-99a-5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD
    Article Snippet: .. The Ect1 (cat. no. CRL-2614), HeLa (cat. no. CRM-CCL-2), C33A (cat. no. HTB-31) and SiHa (cat. no. HTB-35) cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM containing 10% FBS, incubated with 5% CO 2 and 37°C. miR-99a-5p mimic and miR-NC mimic were purchased from Shanghai GenePharma Co., Ltd. Transfection of pcDNA3.1-RRAGD plasmid (30 μg), pcDNA3.1 plasmid (30 μg), miR-99a-5p mimic (100 nM; 5′-AACCCGUAGAUCCGAUCUUGUG-3′) and miR-NC mimic (100 nM; 5′-UUGUACUACACAAAAGUACUG-3′) was performed at 37°C for 48 h using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). ..

    Transfection:

    Article Title: miR-99a-5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD
    Article Snippet: .. The Ect1 (cat. no. CRL-2614), HeLa (cat. no. CRM-CCL-2), C33A (cat. no. HTB-31) and SiHa (cat. no. HTB-35) cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM containing 10% FBS, incubated with 5% CO 2 and 37°C. miR-99a-5p mimic and miR-NC mimic were purchased from Shanghai GenePharma Co., Ltd. Transfection of pcDNA3.1-RRAGD plasmid (30 μg), pcDNA3.1 plasmid (30 μg), miR-99a-5p mimic (100 nM; 5′-AACCCGUAGAUCCGAUCUUGUG-3′) and miR-NC mimic (100 nM; 5′-UUGUACUACACAAAAGUACUG-3′) was performed at 37°C for 48 h using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). ..

    Plasmid Preparation:

    Article Title: miR-99a-5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD
    Article Snippet: .. The Ect1 (cat. no. CRL-2614), HeLa (cat. no. CRM-CCL-2), C33A (cat. no. HTB-31) and SiHa (cat. no. HTB-35) cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM containing 10% FBS, incubated with 5% CO 2 and 37°C. miR-99a-5p mimic and miR-NC mimic were purchased from Shanghai GenePharma Co., Ltd. Transfection of pcDNA3.1-RRAGD plasmid (30 μg), pcDNA3.1 plasmid (30 μg), miR-99a-5p mimic (100 nM; 5′-AACCCGUAGAUCCGAUCUUGUG-3′) and miR-NC mimic (100 nM; 5′-UUGUACUACACAAAAGUACUG-3′) was performed at 37°C for 48 h using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). ..

    other:

    Article Title: MiR-101-3p Suppresses Progression of Cervical Squamous Cell Carcinoma by Targeting and Down-Regulating KPNA2
    Article Snippet: Human cervical squamous epithelial cell line , Ect1 , CRL-2614 , American Type Culture Collection.



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    human ectocervical epithelial cell line ect1 e6e7  (ATCC)
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    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth <t>of</t> <t>Ect1/E6E7</t> cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)
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    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os <t>(Ect1/E6E7)</t> and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.
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    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    doi: 10.3389/fcell.2026.1773562

    Figure Lengend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Article Snippet: HeLa and Ect1 cell lines were obtained from ATCC.

    Techniques: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay