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ect1 cell lines  (ATCC)


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    ATCC ect1 cell lines
    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os <t>(Ect1/E6E7)</t> and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.
    Ect1 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ect1/HeLa/pmc13006679-94-2-8
    Average 99 stars, based on 10464 article reviews
    ect1 cell lines - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer"

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2026.1773562

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.
    Figure Legend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Techniques Used: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay

    Related Articles

    Purification:

    Article Title: A conserved tyrosine motif encodes a structural vulnerability controlling stability and activity across J-domain proteins
    Article Snippet: The human adenocarcinoma epithelial cell line HeLa (ATCC, catalog number: CRM-CCL-2) was obtained from the American Type Culture Collection.. HEK-293 DNAJB1 KO cells were generously provided by Christian Hansen, Ph.D. (Lund University).HEK-293 DNAJB1 KO cells were generously provided by Christian Hansen, Ph.D. (Lund University).

    Article Title: KDM2B controls HIF levels and activity through its JmjC and CxxC domains.
    Article Snippet: blished, w ill replace this version. The m ost up-to-date-version is available at doi.org/10.1042/BC J20260212 13 Reagent resource Reference/source Identifier/catalogue number Experimental models HeLa ATCC CRM-CCL-2 U2OS ATCC HTB-96 MDA-MB-231 ATCC CRM-HTB-26 HEK293 ATCC CRL-1573 HeLa HRE-luciferase Generated by Sonia Rocha lab U2OS HRE-luciferase Generated by Sonia Rocha lab MDA-MB-231 HRElucifera

    Cell Culture:

    Article Title: A conserved tyrosine motif encodes a structural vulnerability controlling stability and activity across J-domain proteins
    Article Snippet: The human adenocarcinoma epithelial cell line HeLa (ATCC, catalog number: CRM-CCL-2) was obtained from the American Type Culture Collection.. HEK-293 DNAJB1 KO cells were generously provided by Christian Hansen, Ph.D. (Lund University).HEK-293 DNAJB1 KO cells were generously provided by Christian Hansen, Ph.D. (Lund University).

    Article Title: KDM2B controls HIF levels and activity through its JmjC and CxxC domains.
    Article Snippet: blished, w ill replace this version. The m ost up-to-date-version is available at doi.org/10.1042/BC J20260212 13 Reagent resource Reference/source Identifier/catalogue number Experimental models HeLa ATCC CRM-CCL-2 U2OS ATCC HTB-96 MDA-MB-231 ATCC CRM-HTB-26 HEK293 ATCC CRL-1573 HeLa HRE-luciferase Generated by Sonia Rocha lab U2OS HRE-luciferase Generated by Sonia Rocha lab MDA-MB-231 HRElucifera



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    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os <t>(Ect1/E6E7)</t> and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.
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    Image Search Results


    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    doi: 10.3389/fcell.2026.1773562

    Figure Lengend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Article Snippet: HeLa and Ect1 cell lines were obtained from ATCC.

    Techniques: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay