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eclipse ni e upright fluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse ni e upright fluorescence microscope
    Eclipse Ni E Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ni-e+fluorescence+microscope/Objectives/pmc12863670-72-9-8
    Average 99 stars, based on 60152 article reviews
    eclipse ni e upright fluorescence microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Microscopy:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Fluorescence:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Electron Microscopy:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Immunohistochemical staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Confocal:

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Single Cell:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Clone Assay:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Process/Product Development:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..



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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    Image Search Results


    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: Staining, Immunostaining, Immunofluorescence, Imaging, Two Tailed Test

    a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: In Vitro, Inhibition, Immunostaining, Staining, Immunofluorescence, Two Tailed Test