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eclipse ni e light microscope  (Nikon)


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    Structured Review

    Nikon eclipse ni e light microscope
    Eclipse Ni E Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ni-e+fluorescence+microscope/DS-Ri2/pmc08369064-85-22-21
    Average 99 stars, based on 1 article reviews
    eclipse ni e light microscope - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Light Microscopy:

    Article Title: Sex-specific skeletal muscle fiber atrophy and reduced macrophage inflammation in a rodent transverse aortic constriction model.
    Article Snippet: .. After 24 h, the center of the wells was visualized under a light microscope at 200X magnification and imaged (DS-Ri2 Nikon Eclipse Ti-U, Nikon). ..

    Microscopy:

    Article Title: The unorthodox aquaporin-12L ortholog is essential for oogenesis in the crustacean Artemia franciscana
    Article Snippet: .. Sections were mounted with Eukitt quick-hardening mounting medium (Sigma–Aldrich, #03989), and images were acquired with an Eclipse 90i microscope equipped with a Nikon DS-Ri2 camera (Nikon, Tokio, Japan). ..

    Article Title: Uncovering hidden diversity in Didymellaceae from Yunnan: Introducing three novel species of Didymella and Nothophoma
    Article Snippet: Ascomata/conidiomata visualized on the host surface were observed under an Olympus SZ61 stereomicroscope (Model SZ2-ILST; Olympus Corporation, Tokyo, Japan) and photographed by a digital camera. .. Micro-morphological features were examined under a Nikon ECLIPSE Ni-U compound microscope with DIC microscopy and images captured with a Nikon DS-Ri2 camera (Nikon Instruments Inc., Tokyo, Japan). ..

    Article Title: Scaevola taccada as a keystone nesting resource for sympatric cavity-nesting bees and its implications for coastal biodiversity conservation
    Article Snippet: Photographs of twigs and broods were taken using a Canon 5D Mark IV (Tokyo, Japan) camera mounted with a 100 mm macro lens. .. Multifocal images of mounted specimens were produced using a Nikon SMZ25 microscope with a Nikon DS-Ri 2 digital camera system (Melville, NY, USA). ..

    Article Title: Full-Length Transcriptome Analysis Identifies Candidate Molecular Features Associated with Golden-Lip Coloration in Pinctada fucata martensii .
    Article Snippet: .. After final PBS washes, sections were mounted using antifade mounting medium (AR1109, Boster, Wuhan, China) and observed using a Nikon ECLIPSE Ni fluorescence microscope (Nikon Corporation, Tokyo, Japan) equipped with a DS-Ri2 camera (Nikon Corporation, Tokyo, Japan). ..

    Article Title: Multi-level toxicity of 6PPD-Q and TiO 2 nanoparticles in Mytilus coruscus: Links among neurochemical, circadian and metabolic responses.
    Article Snippet: Tire-derived 6PPD-quinone (6PPD-Q) and titanium dioxide nanoparticles (TiO2 NPs) are increasingly detected in aquatic environments, yet their combined effects on marine bivalves remain poorly understood.. In this study, the thick-shell mussel Mytilus coruscus was exposed to 6PPD-Q, TiO2 NPs, and their mixtures to evaluate multi-level physiological, biochemical, histological, and molecular responses.. The results demonstrated that 6PPD-Q and TiO2 NPs, particularly under high-concentration co-exposure, significantly impaired mussel physiological performance, as indicated by reduced clearance rate and O:N ratio, increased oxygen consumption and excretion rate, and pronounced gill histopathological injury.

    Article Title: Serum Vitamin D Levels at Birth Are Not Associated With the Formation of Interglobular Dentin in Deciduous Teeth of Modern New Zealand Children.
    Article Snippet: .. Samples were viewed under transmitted light on a Nikon Ni- U microscope equipped with a DS- Ri2 camera. ..

    Software:

    Article Title: Comparative Analysis of Cytoskeletal Proteins and the Sarcoglycan Complex in the Cremaster Muscle of Patients with Retractile and Undescended Testis.
    Article Snippet: The sections were 94 then processed with routinary histological techniques and stained with hematoxylin and 95 eosin (H&E) and examined under a Light microscope Eclipse Ci-L (Nikon Corporation, Tokyo, 96 Japan). .. The micrographs were acquired with a Nikon DS-Ri2 Camera, equipped with a NIS-97 Element F Software. ..

    Produced:

    Article Title: Scaevola taccada as a keystone nesting resource for sympatric cavity-nesting bees and its implications for coastal biodiversity conservation
    Article Snippet: Photographs of twigs and broods were taken using a Canon 5D Mark IV (Tokyo, Japan) camera mounted with a 100 mm macro lens. .. Multifocal images of mounted specimens were produced using a Nikon SMZ25 microscope with a Nikon DS-Ri 2 digital camera system (Melville, NY, USA). ..

    Fluorescence:

    Article Title: Full-Length Transcriptome Analysis Identifies Candidate Molecular Features Associated with Golden-Lip Coloration in Pinctada fucata martensii .
    Article Snippet: .. After final PBS washes, sections were mounted using antifade mounting medium (AR1109, Boster, Wuhan, China) and observed using a Nikon ECLIPSE Ni fluorescence microscope (Nikon Corporation, Tokyo, Japan) equipped with a DS-Ri2 camera (Nikon Corporation, Tokyo, Japan). ..

    Transmitted Light:

    Article Title: Serum Vitamin D Levels at Birth Are Not Associated With the Formation of Interglobular Dentin in Deciduous Teeth of Modern New Zealand Children.
    Article Snippet: .. Samples were viewed under transmitted light on a Nikon Ni- U microscope equipped with a DS- Ri2 camera. ..



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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.
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    Image Search Results


    a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: Staining, Immunostaining, Immunofluorescence, Imaging, Two Tailed Test

    a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Journal: Nature Communications

    Article Title: Cellular crosstalk mediated by TGF-β drives epithelial-mesenchymal transition in patient-derived multi-compartment biliary organoids

    doi: 10.1038/s41467-025-61442-5

    Figure Lengend Snippet: a Overview of in vitro A8301-mediated TGF-β signal inhibition in BA-MBOs. Created in BioRender. Ayabe, H. (2025) https://BioRender.com/q1pk62z . b Representative plots and quantification of FACS of day 14 MBOs treated with A8301. Number of hybrid (pan-CK + /VIM + ), cholangiocyte (pan-CK + /VIM - ) and mesenchymal (pan-CK − /VIM + ) cells were quantified (Mean ± SD; n = 12 organoids). c Images of MBOs treated with A8301 captured before collection and after whole-mount immunostaining (CK19: red, CD31: green, VIM: white). Abundance of CK19 + structures (Mean ± SD; n = 9 organoids examined over 3 independent experiments) and size distribution of PBG-like structures were quantified ( n = 39-104 clusters examined over 3 independent experiments). d Immunostaining of ECAD (green), CK19 (red) and Nucleus (blue; DAPI) in MBOs treated with A8301. ECAD + cells in CK19 + cells in the field were quantified (Mean ± SD; n = 12 fields from 3 organoids). e Immunostaining of ZO1 (green), CK19 (red) and Nucleus (blue; DAPI) in MBO treated with A8301. ZO1 + cells in CK19 + cells per field were quantified (Mean ± SD; n = 12 fields from 3 organoids). f Alcian blue staining of MBOs treated with A8301. Mucin production was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined immunofluorescence data) areas (Mean ± SD; n = 12 fields from 3 organoids). g Top enriched terms of MSigDB Hallmark 2020 , and GO , sets in serum proteins (reanalyzed from Lertudomphonwanit et al. ) upregulated in BA subjects ( n = 175 patients) vs age-matched normal controls ( n = 9). MF: Molecular Function, BP: Biological Process. h Heatmap of enriched proteins in the EMT pathway in MSigDB Hallmark 2020 sets. ( n = 9 NC and 175 BA subjects). i Serum TGF-β/Activin ligand levels represented by the relative fluorescent unit (RFU; Mean ± SD). ( n = 9 NC and 175 BA subjects). The p -values were shown in plots and were determined using one-way ANOVA with Tukey’s test ( b – f ) and two-tailed Welch’s t -test ( i ).

    Article Snippet: Bright field images were captured using a light microscope (Olympus, BX43) and immunofluorescence images were captured using either fluorescence (Olympus, BX51; Nikon, ECLIPSE Ni-E) or confocal (Nikon, ECLIPSE Ti; Zeiss, LSM880) microscopes.

    Techniques: In Vitro, Inhibition, Immunostaining, Staining, Immunofluorescence, Two Tailed Test