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bigdye labeled dye-terminator chemistry  (Thermo Fisher)


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    Thermo Fisher bigdye labeled dye-terminator chemistry
    Bigdye Labeled Dye Terminator Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dye-labeled+terminators/pmc01277808-45-12-16
    Average 90 stars, based on 1 article reviews
    bigdye labeled dye-terminator chemistry - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: A survey of Rhipicephalus microplus populations for mutations associated with pyrethroid resistance.
    Article Snippet: Mutations associated with pyrethroid resistance were found in Mexican strains of Rhipicephalus microplus (Canestrini).. A mutation in the sodium channel gene was reported in strains highly resistant to permethrin and another mutation in an esterase gene in a strain that shows moderate resistance to the same pesticide.. Methods based on the melting temperature difference of ampliÞed allele-speciÞc DNA fragments were developed that can detect these mutations rapidly in individual larvae.

    Article Title: Multigene phylogenetic and population differentiation data confirm the existence of a cryptic species within Chrysoporthe cubensis.
    Article Snippet: .. PCR products were sequenced using BigDye dye terminator chemistry (Applied Biosystems, USA) and an ABI Prism 3500 automated sequencing machine (Applied Biosystems). ..

    Article Title: Accounting for female reproductive cycles in a superpopulation capture-recapture framework.
    Article Snippet: Briefly, the primers dlp1.5 (Baker et al. 1998) and tphe (Carroll et al. 2011a), both modified with a 50-M13 primer extension to facilitate subsequent sequencing reactions, were used to amplify ;950 bp of the mtDNA control region. .. PCR products were purified for sequencing with ExoSAP-IT (USB, Cleveland, Ohio, USA) and sequenced using BigDye Dye Terminator Chemistry (Applied Biosystems, Foster City, California, USA) on an ABI 3730 or ABI 3130 (Applied Biosystems). .. Sequences were aligned and edited in Sequencher v4.2 (Gene Codes Corporation, Ann Arbor, Michigan, USA) or Geneious (Drummond et al. 2006), and haplotypes were identified from a 500 bp consensus region using haplotype codes established by Carroll et al. (2011a).

    Article Title: Porcine kallikrein gene family: genomic structure, mapping, and differential expression analysis.
    Article Snippet: .. The PCR products generated through vectorette PCR were treated with shrimp alkaline phosphatase (1 U μl−1) and Exonuclease III (10 U μl−1) at a volume equaling 5% of the PCR product and incubated at 37 °C for 30 min followed by 80 °C for 10 min. Sequencing reactions were performed in a 10-μl reaction volume containing 2 μl PCR product, 1 mM sequencing primer, 2 μl of BigDye dye terminator mix (Applied Biosystems), and 1 μl buffer (400 mM Tris, pH 9, 10 mMMgCl2). ..

    other:

    Article Title: A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease
    Article Snippet: DNA sequencing was performed on Applied Biosystems automated DNA sequencers (3100) using BigDye labeled dye-terminator chemistry (Applied Biosystems).

    Article Title: Novel COL4A5, COL4A4, and COL4A3 mutations in Alport syndrome.
    Article Snippet: For direct sequencing, the BigDye® dye terminator method (Applied Biosystems, Foster City, USA) was used.

    Polymerase Chain Reaction:

    Article Title: Multigene phylogenetic and population differentiation data confirm the existence of a cryptic species within Chrysoporthe cubensis.
    Article Snippet: .. PCR products were sequenced using BigDye dye terminator chemistry (Applied Biosystems, USA) and an ABI Prism 3500 automated sequencing machine (Applied Biosystems). ..

    Article Title: Accounting for female reproductive cycles in a superpopulation capture-recapture framework.
    Article Snippet: Briefly, the primers dlp1.5 (Baker et al. 1998) and tphe (Carroll et al. 2011a), both modified with a 50-M13 primer extension to facilitate subsequent sequencing reactions, were used to amplify ;950 bp of the mtDNA control region. .. PCR products were purified for sequencing with ExoSAP-IT (USB, Cleveland, Ohio, USA) and sequenced using BigDye Dye Terminator Chemistry (Applied Biosystems, Foster City, California, USA) on an ABI 3730 or ABI 3130 (Applied Biosystems). .. Sequences were aligned and edited in Sequencher v4.2 (Gene Codes Corporation, Ann Arbor, Michigan, USA) or Geneious (Drummond et al. 2006), and haplotypes were identified from a 500 bp consensus region using haplotype codes established by Carroll et al. (2011a).

    Article Title: Porcine kallikrein gene family: genomic structure, mapping, and differential expression analysis.
    Article Snippet: .. The PCR products generated through vectorette PCR were treated with shrimp alkaline phosphatase (1 U μl−1) and Exonuclease III (10 U μl−1) at a volume equaling 5% of the PCR product and incubated at 37 °C for 30 min followed by 80 °C for 10 min. Sequencing reactions were performed in a 10-μl reaction volume containing 2 μl PCR product, 1 mM sequencing primer, 2 μl of BigDye dye terminator mix (Applied Biosystems), and 1 μl buffer (400 mM Tris, pH 9, 10 mMMgCl2). ..

    Purification:

    Article Title: Accounting for female reproductive cycles in a superpopulation capture-recapture framework.
    Article Snippet: Briefly, the primers dlp1.5 (Baker et al. 1998) and tphe (Carroll et al. 2011a), both modified with a 50-M13 primer extension to facilitate subsequent sequencing reactions, were used to amplify ;950 bp of the mtDNA control region. .. PCR products were purified for sequencing with ExoSAP-IT (USB, Cleveland, Ohio, USA) and sequenced using BigDye Dye Terminator Chemistry (Applied Biosystems, Foster City, California, USA) on an ABI 3730 or ABI 3130 (Applied Biosystems). .. Sequences were aligned and edited in Sequencher v4.2 (Gene Codes Corporation, Ann Arbor, Michigan, USA) or Geneious (Drummond et al. 2006), and haplotypes were identified from a 500 bp consensus region using haplotype codes established by Carroll et al. (2011a).

    DNA Extraction:

    Article Title: G protein-coupled receptor-like receptors and modulators thereof
    Article Snippet: PCR products were typically gel-purified using QIAquick columns (Qiagen, Valencia, Calif.) and either cloned directly into pCR3.1 (Invitrogen, Carlsbad, Calif.), or indirectly through an initial cloning step into pCR2.1. .. Plasmids containing the appropriate inserts were prepared by a mini-prep DNA isolation procedure (Qiagen) and were sequenced using BigDye dye terminator chemistry (PE Applied Biosystems, Foster City, Calif.) on an ABI 377 automated DNA sequencer, as described above. .. Complete sequences were assembled using Sequencher software (Version 3.0, GeneCodes, Ann Arbor, Mich.) and compared against the expected sequence predicted from the Wormpep database.

    Generated:

    Article Title: Porcine kallikrein gene family: genomic structure, mapping, and differential expression analysis.
    Article Snippet: .. The PCR products generated through vectorette PCR were treated with shrimp alkaline phosphatase (1 U μl−1) and Exonuclease III (10 U μl−1) at a volume equaling 5% of the PCR product and incubated at 37 °C for 30 min followed by 80 °C for 10 min. Sequencing reactions were performed in a 10-μl reaction volume containing 2 μl PCR product, 1 mM sequencing primer, 2 μl of BigDye dye terminator mix (Applied Biosystems), and 1 μl buffer (400 mM Tris, pH 9, 10 mMMgCl2). ..

    Incubation:

    Article Title: Porcine kallikrein gene family: genomic structure, mapping, and differential expression analysis.
    Article Snippet: .. The PCR products generated through vectorette PCR were treated with shrimp alkaline phosphatase (1 U μl−1) and Exonuclease III (10 U μl−1) at a volume equaling 5% of the PCR product and incubated at 37 °C for 30 min followed by 80 °C for 10 min. Sequencing reactions were performed in a 10-μl reaction volume containing 2 μl PCR product, 1 mM sequencing primer, 2 μl of BigDye dye terminator mix (Applied Biosystems), and 1 μl buffer (400 mM Tris, pH 9, 10 mMMgCl2). ..

    Recombinant:

    Article Title: G protein-coupled receptor-like receptors and modulators thereof
    Article Snippet: .. Analysis of the GPCR-Like Receptor Sequences DNA from pCR2.1 recombinant plasmids containing the expected insert size were sequenced using BigDye dye terminator chemistry (PE Applied Biosystems, Foster City, Calif.) on an ABI 377 automated DNA sequencer. .. Complete sequences were assembled using Sequencher software (GeneCodes, Ann Arbor, Mich.) and compared against the expected sequence as predicted from Wormpep.



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