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Kaneka Corp takyon no rox sybr 2x mastermix blue dttp
Takyon No Rox Sybr 2x Mastermix Blue Dttp, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dttp/mastermix+sybr+takyon/pm41802669-48-29-37
Average 86 stars, based on 1 article reviews
takyon no rox sybr 2x mastermix blue dttp - by Bioz Stars, 2026-10
86/100 stars

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Quantitative RT-PCR:

Article Title: Epigenetic regulation of a heat-trainable small heat shock protein gene locus controls thermomemory in a unicellular alga
Article Snippet: DNase treatment and cDNA synthesis were performed using DNase I, RNase-free (Thermo Scientifc) and the RevertAid First Strand cDNA synthesis kit (Thermo Fisher Scientific) with oligo(dT) primers: 43 + 44 ( CMJ099C ), 45 + 46 ( CMJ100C ), 47 + 48 ( CMJ101C ), 49 + 50 ( CMJ102C ), 51 + 52 ( CMS262C ), 53 + 54 ( CMN304C ), 55 + 56 ( CMM193C ,Table S5). .. RT-qPCR was performed with 10 ng cDNA using the TakyonTM ROX SYBR 2X MasterMix dTTP blue (Eurogentec) according to the manufacturer’s instructions on the QuantStudio5 (Applied Biosystems). ..

Real-time Polymerase Chain Reaction:

Article Title: Survival of a potential bacterial biocontrol agent in the soil and its impact on microbial communities.
Article Snippet: DNA extracts were then quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, France) and normalized to 0.5 ng μL−1. .. 2.7 qPCR quantification of the biocontrol strain SCL1 SCL1 gene copy number abundancewasmeasured by qPCR using strain-specific primers targeting a single-copy gene.22 qPCR was performed on a Viia7 in 15-μL reactions comprising 7.5 μL of master mix (Takyon lowROX SYBR, Eurogentec, France), 0.15 μL of each primer (10 μM), 3.85 μL of MilliQ water, 0.05 μL of T4 Gene 32 protein (Mpbio, France) and 2 μL of DNA (0.5 ng μL−1). ..

Article Title: Increases in BCL2L1 and ID1 dosage synergistically drive fate bias and competitive advantage in human pluripotent stem cells
Article Snippet: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen), quantified via NanoDrop 1000 (Thermo Fisher), and stored at 4°C. .. For qPCR, reactions (20 μL total volume) contained 10 μL TaqMan 2× MasterMix Plus–Low ROX (Eurogentec), 1 μL TaqMan Copy Number Assay (ID1 [20q], KIF14 [1q], and NANOG [12p]; Table S2), 1 μL RNaseP reference assay (Applied Biosystems), 40 ng DNA, and nuclease-free water. .. Cycling conditions were: 2 min at 50°C; 40 cycles of 95°C for 10 min, 95°C for 15 sec, and 60°C for 1 min. hPSC DNA with known karyotype and non-template controls were included, with samples run in triplicates on a ViiA7 system (Thermo Fisher).

TaqMan Copy Number Assay:

Article Title: Increases in BCL2L1 and ID1 dosage synergistically drive fate bias and competitive advantage in human pluripotent stem cells
Article Snippet: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen), quantified via NanoDrop 1000 (Thermo Fisher), and stored at 4°C. .. For qPCR, reactions (20 μL total volume) contained 10 μL TaqMan 2× MasterMix Plus–Low ROX (Eurogentec), 1 μL TaqMan Copy Number Assay (ID1 [20q], KIF14 [1q], and NANOG [12p]; Table S2), 1 μL RNaseP reference assay (Applied Biosystems), 40 ng DNA, and nuclease-free water. .. Cycling conditions were: 2 min at 50°C; 40 cycles of 95°C for 10 min, 95°C for 15 sec, and 60°C for 1 min. hPSC DNA with known karyotype and non-template controls were included, with samples run in triplicates on a ViiA7 system (Thermo Fisher).

Amplification:

Article Title: Screening for carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in freshwater, bathing water and hospital continuum in Belgium.
Article Snippet: .. For amplification on a CFX96 thermocycler (Bio-Rad, Marnes-la-Coquette, France), 5 μl of DNA extract was mixed with 1,5 μl of each primer (final concentration : 0.6 μM), 12 μl Takyon No ROX SYBR 2x MasterMix blue dTTP (Eurogentec, Liège, Belgium) and 5 μl of DNase free water in a final volume of 25 μl. ..

Concentration Assay:

Article Title: Screening for carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in freshwater, bathing water and hospital continuum in Belgium.
Article Snippet: .. For amplification on a CFX96 thermocycler (Bio-Rad, Marnes-la-Coquette, France), 5 μl of DNA extract was mixed with 1,5 μl of each primer (final concentration : 0.6 μM), 12 μl Takyon No ROX SYBR 2x MasterMix blue dTTP (Eurogentec, Liège, Belgium) and 5 μl of DNase free water in a final volume of 25 μl. ..

Article Title: Sexing chicken embryos by real-time PCR using primers for the KCMF gene located on the W chromosome
Article Snippet: Real-time PCR was performed in 384-well plates using 2XMaster Mix Takyon and LightCycler® 480 instrument II (Roche, Basel, Switzerland). .. Each 10 μL reaction volume contained 2.5 μL of sample, 2.25 μL nuclease-free water, 0.125 μL of each forward and reverse KCMF1 primers (initial concentration: 10 μM) and 5 μL 2X Master Mix Takyon (Eurogentec, UF-NSMT-B0701) containing SyberGreen 1 as the fluorescent probe. ..

other:




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