Journal: iScience

Article Title: TMEM106A inhibits enveloped virus release from cell surface

doi: 10.1016/j.isci.2022.103843

Figure Lengend Snippet:

Article Snippet: 32 P-dTTP , Perkin Elmer , NEG005H250UC.

Techniques: Virus, Recombinant, Transfection, Staining, Luciferase, Lysis, Reporter Assay, SYBR Green Assay, Produced, Control, shRNA, Sequencing, Software

Journal: Mitochondrion

Article Title: Splitting the functions of Rim2, a mitochondrial iron/pyrimidine carrier

doi: 10.1016/j.mito.2018.12.005

Figure Lengend Snippet: Isolated mitochondria, from strain YS102–46 (mrs3Δ mrs4Δ GAL-RIM2) transformed with YEp351 (Vec), and YEp351 bearing: Rim2 (WT), Rim2 (E248A), Rim2 (K299A) and grown in the absence of galactose, were preloaded with 3H-dTTP. The 3H-dTTP was washed away and the mitochondria were resuspended in HS buffer containing 50 mM NaCl to which 100 μm TDP, 100 μM TTP or nothing (−) had been added. After 10 min incubation at room temperature, the mitochondria were pelleted and 3H-TTP exported to the supernatant was quantified by liquid scintillation counting. (A) Results are expressed as a percentage of the total 3H-TTP initially loaded into the mitochondria, with 3H-dTTP remaining in mitochondria (dark gray) or 3H-dTTP exchanged to supernatant (light gray). Error bars indicate SD (n=3). (B) A single typical exchange experiment is shown with results expressed as cpm 3H-TTP retained in mitochondria (dark gray) or exported to the supernatant (light gray).

Article Snippet: Intact mitochondria in HS buffer containing 50 mM NaCl were incubated with 260 nM tritiated TTP (thymidine 5’ triphosphate [methyl 3 H] tetra sodium salt, MP Biomedical) or 48 nM dTTP (deoxy-thymidine 5’ triphosphate [methyl 3 H] tetra sodium salt, MP Biomedical) for 5 min at room temperature.

Techniques: Isolation, Transformation Assay, Incubation