draq5 (Biostatus)
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Draq5, supplied by Biostatus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5/draq5/pmc06728451-52-30-34
Average 90 stars, based on 1 article reviews
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other:Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice Article Snippet: Samples were blocked with 10% normal goat serum in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table at 4 °C overnight, followed by an incubation with dye-conjugate secondary antibodies (Supplementary Table ) at room temperature for 1 h. Nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole or Article Title: Injured tubule derived CCN1 exacerbates renal congestion-mediated acute kidney injury and fibrosis. Article Snippet: Samples were then blocked with 3% bovine serum albumin (BSA) (Nacalai Tesque, Kyoto, Japan) in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table S2 at 4 °C overnight, followed by an incubation with the dye-conjugate secondary antibodies shown in Supplementary Table S2 at room temperature for 1 h. Nuclear counterstaining was performed using DAPI or Article Title: Injured tubule derived CCN1 exacerbates renal congestion-mediated acute kidney injury and fibrosis Article Snippet: Samples were then blocked with 3% bovine serum albumin (BSA) (Nacalai Tesque, Kyoto, Japan) in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table at 4 °C overnight, followed by an incubation with the dye-conjugate secondary antibodies shown in Supplementary Table at room temperature for 1 h. Nuclear counterstaining was performed using DAPI or Article Title: Protein-based adhesive and its modification Article Snippet: Finally, coverslips were rinsed twice in PBS, mounted with VECTASHIELD® (Vector Laboratories), and sealed with nail polish. Article Title: Differential regulation of MMP activity by TGFβ1 in fast- and slow- twitch muscle repair: insights from EDL and soleus muscle-derived myoblasts Article Snippet: The nuclei were visualized with Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice. Article Snippet: Samples were blocked with 10% normal goat serum in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table S1 at 4 °C overnight, followed by an incubation with dye-conjugate secondary antibodies (Supplementary Table S1) at room temperature for 1 h. Nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole or FACS:Article Title: Human airway submucosal gland organoids to study respiratory inflammation and infection. Article Snippet: The cells were then washed with ice-cold AdDMEM/F12 (Thermo Fisher Scientific) and resuspended in culture medium supplemented with Y-27632 dihydrochloride (10 μM, Abmole Bioscience). .. After filtering them into FACS tubes through a cell strainer (Falcon), cells were co-stained with DAPI (Sigma-Aldrich) and Staining:Article Title: Optimized mini-dystrophin genes and expression cassettes and their use Article Snippet: Alexa Fluor 555 wheat germ agglutinin (WGA) conjugate (1:500, Molecular Probes, Eugene, OR) was used to visualize connective tissue. .. Nuclei were stained with |

![(A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with <t>Draq5</t> (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_37/10__64898_slash_2026__02__16__706237/10__64898_slash_2026__02__16__706237___F1.large.jpg)