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Biostatus draq5
Draq5, supplied by Biostatus, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/draq5/draq5/pmc06728451-52-30-34
Average 90 stars, based on 1 article reviews
draq5 - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

other:

Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice
Article Snippet: Samples were blocked with 10% normal goat serum in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table at 4 °C overnight, followed by an incubation with dye-conjugate secondary antibodies (Supplementary Table ) at room temperature for 1 h. Nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole or DRAQ5 (DR50050; BioStatus, Leicestershire, UK; 1:2000), followed by mounting in Prolong-Gold (Thermo Fisher Scientific, Waltham, MA).

Article Title: Injured tubule derived CCN1 exacerbates renal congestion-mediated acute kidney injury and fibrosis.
Article Snippet: Samples were then blocked with 3% bovine serum albumin (BSA) (Nacalai Tesque, Kyoto, Japan) in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table S2 at 4 °C overnight, followed by an incubation with the dye-conjugate secondary antibodies shown in Supplementary Table S2 at room temperature for 1 h. Nuclear counterstaining was performed using DAPI or DRAQ5 (DR05500; BioStatus, Leicestershire, UK; 1:200), followed by mounting in Prolong-Gold (Thermo Fisher Scientific, Waltham, MA).

Article Title: Injured tubule derived CCN1 exacerbates renal congestion-mediated acute kidney injury and fibrosis
Article Snippet: Samples were then blocked with 3% bovine serum albumin (BSA) (Nacalai Tesque, Kyoto, Japan) in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table at 4 °C overnight, followed by an incubation with the dye-conjugate secondary antibodies shown in Supplementary Table at room temperature for 1 h. Nuclear counterstaining was performed using DAPI or DRAQ5 (DR05500; BioStatus, Leicestershire, UK; 1:200), followed by mounting in Prolong-Gold (Thermo Fisher Scientific, Waltham, MA).

Article Title: Protein-based adhesive and its modification
Article Snippet: Finally, coverslips were rinsed twice in PBS, mounted with VECTASHIELD® (Vector Laboratories), and sealed with nail polish.


Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice.
Article Snippet: Samples were blocked with 10% normal goat serum in PBS and sequentially incubated with the primary antibodies shown in Supplementary Table S1 at 4 °C overnight, followed by an incubation with dye-conjugate secondary antibodies (Supplementary Table S1) at room temperature for 1 h. Nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole or DRAQ5 (DR50050; BioStatus, Leicestershire, UK; 1:2000), followed by mounting in Prolong-Gold (Thermo Fisher Scientific, Waltham, MA).

FACS:

Article Title: Human airway submucosal gland organoids to study respiratory inflammation and infection.
Article Snippet: The cells were then washed with ice-cold AdDMEM/F12 (Thermo Fisher Scientific) and resuspended in culture medium supplemented with Y-27632 dihydrochloride (10 μM, Abmole Bioscience). .. After filtering them into FACS tubes through a cell strainer (Falcon), cells were co-stained with DAPI (Sigma-Aldrich) and DRAQ5 (Biostatus), and subjected to FACS sorting for live cells. .. Pre-warmed Collagenase (Sigma-Aldrich) diluted in digestion buffer was added to the tissue pieces to a final concentration of 1 mg/mL, followed by incubation at 37 ◦ C for 15 minutes.

Staining:

Article Title: Optimized mini-dystrophin genes and expression cassettes and their use
Article Snippet: Alexa Fluor 555 wheat germ agglutinin (WGA) conjugate (1:500, Molecular Probes, Eugene, OR) was used to visualize connective tissue. .. Nuclei were stained with DRAQ5 (1:1000, BioStatus Ltd, Shepshed, UK). ..



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Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with <t>DRAQ5</t> (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).
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Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with <t>DRAQ5</t> (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).
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Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with <t>DRAQ5</t> (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).
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Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with <t>DRAQ5</t> (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).
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Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with <t>DRAQ5</t> (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).
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Miltenyi Biotec draq5 staining solution
(A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with <t>Draq5</t> (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.
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Image Search Results


Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with DRAQ5 (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).

Journal: Journal of Functional Biomaterials

Article Title: Influence of Micro-Nanostructured Anatase-Coated SLA Titanium on Macrophage Behavior

doi: 10.3390/jfb17030111

Figure Lengend Snippet: Behavior of M0-, M1-, and M2-MDMs on differently modified titanium Surfaces. ( A ) Representative CLSM images showing M0-, M1-, and M2-MDMs on coverslip, Ti-machined, Ti-SLA, and Ti-SLA-anatase surfaces after 48 h of incubation. The cytoskeleton (F-actin) was stained with FITC-Phalloidin (green), and nuclei were stained with DRAQ5 (purple). ( B ) Representative SEM images of M0-, M1, and M2 macrophages on coverslip and titanium surface modifications. ( C ) Metabolic activity was assessed using the CCK-8 assay, with values normalized to coverslip M0 (set as 100%). Data are presented as mean ± SEM. n = 3. No significant differences were observed between groups (Friedman test with Dunn’s multiple comparisons test).

Article Snippet: Samples were blocked in 1% bovine serum albumin (BSA; VWR International, Leuven, Belgium; Cat No. 422361V) and 0.05% Tween-20 (Roth, Karlsruhe, Germany; Cat. No. 9127.1) in PBS for 30 min. Cytoskeletal staining was performed using 5 μg/mL phalloidin–FITC (Sigma Aldrich, Saint Louis, MI, USA; Cat. No. P5282) in blocking buffer for 1 h, followed by nuclear staining with 10 μM DRAQ5 (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat No. 130-117-344) for 15 min at RT.

Techniques: Modification, Incubation, Staining, Activity Assay, CCK-8 Assay

(A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with Draq5 (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.

Journal: bioRxiv

Article Title: Cholesterol remodels the endoplasmic reticulum to control myofibroblastic CAF function

doi: 10.64898/2026.02.16.706237

Figure Lengend Snippet: (A) Measurement of the relative incorporation of [U- 14 C]glucose into protein, non-polar, RNA, DNA and polar fractions following 24 hours of labelling, n=3. (B) Relative incorporation of [1- 14 C]acetate into the lipid fraction of NFs and CAFs, n=3. (C) Gene Set Enrichment Analysis (GSEA) plot derived from RNA-Seq analysis of CAFs versus NFs, showing enrichment of a “lipid metabolic process” signature (GO:0006629). (D) Heatmap of cholesterol biosynthesis gene expression in NFs and CAFs, as determined by RNA-Seq analysis, n=3. (E) Quantification of relative total cholesterol levels in NFs and CAFs, n=3. (F) UMAP visualisation of 19,601 patient-derived breast tumour stromal cells analysed by single cell RNA-Seq (scRNA-Seq) from the Human Breast Cancer Atlas . Stromal clusters corresponding to immunomodulatory CAFs (iCAFs), myCAFs, perivascular (PVL) and endothelial cell subsets are indicated by colour (top). Feature plots illustrating expression of SQLE (bottom left) and SC5D (bottom right) in breast tumour stromal cell clusters. Gene expression is represented by log-normalised expression values. The myCAF cluster is delineated by a dashed line. (G) Representative filipin staining (green) of free cholesterol in NFs and CAFs. Nuclei are stained with Draq5 (blue). Dashed boxes indicate magnified regions shown to the right. White scale bar represents 10 µm, yellow scale bar represents 5 µm. (H) Quantification of relative cholesterol levels in the ER-enriched fraction isolated from NFs and CAFs, n=3.

Article Snippet: Filipin was removed, and cells were washed and imaged in PBS containing 5 μM Draq5 staining solution (Miltenyi Biotec) using a Zeiss Elyra PS.1 confocal microscope (Zeiss) equipped with a 63X/1.4 oil DIC objective.

Techniques: Derivative Assay, RNA Sequencing, Gene Expression, Single Cell, Expressing, Staining, Isolation