af2254 (R&D Systems)
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af2254
Af2254, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dner/Mouse+DNER+Antibody/pmc12821072-6-0-4
Average 93 stars, based on 21 article reviews
Af2254, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dner/Mouse+DNER+Antibody/pmc12821072-6-0-4
Average 93 stars, based on 21 article reviews
af2254 - by Bioz Stars,
2026-11
93/100 stars
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Control:Article Title: Biotin–NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes Article Snippet: Wells were then washed once with PBS (pH 7.4), and protein A was exposed to recombinant purified 100 nM mouse ICAM-1-IgGFc chimera (R&D Systems, Abingdon, U.K.) for 2 h at 37 °C, and finally the wells were blocked with 1.5% BSA in PBS for 30 min at room temperature and washed once with PBS before use in a binding assay. .. As a control, a Immunohistochemistry:Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity Article Snippet: .. For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing ( Article Title: Early downregulation of HC-specific genes in the vestibular sensory epithelium during chronic ototoxicity Article Snippet: .. For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25-6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing ( Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs Article Snippet: 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Membrane:Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity Article Snippet: .. For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing ( Article Title: Early downregulation of HC-specific genes in the vestibular sensory epithelium during chronic ototoxicity Article Snippet: .. For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25-6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing ( Immunofluorescence:Article Title: Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts. Article Snippet: .. The following antibodies were used for immunofluorescence: goat antiPDGFRA (1:1000, AF1062, R&D Systems), rat anti-PDGFRA (1:1000, 14-1401-82, eBioscience), rat anti-cadherin-4 (CDH4, 1:20, MRCD5, Developmental Studies Hybridoma Bank), rat anti-PDGFRB (1:1000, 14-1402-82, eBioscience), goat anti-PDGFRB (1:1000, AF1042, R&D Systems), goat anti-NOTCH3 (1:500, AF1308, R&D Systems), rabbit antiMEOX2 (1:1000, NBP2-30647, Novus Biological), rabbit anti-cleaved CASP3 (1:500, 9661, Cell Signaling), goat anti-PI16 (1:1000, AF4929, R&D Systems), rat anti-CD45 (1:2000, 14-0451-81, eBioscience), Alexa488-conjugated mouse anti-ACTA2 (1:1000, sc-32251 AF488, Santa Cruz Biotechnology), Cy3-conjugated mouse anti-ACTA2 (1:1000, C6198, Sigma), Alexa647-conjugated mouse anti-ACTA2 (1:1000, sc-32251 AF647, Santa Cruz Biotechnology), rat anti-RAGE (1:1000, MAB1179, R&DSystems), rabbit anti-TAGLN (1:1000, ab14106, Abcam), chicken anti-GFP (1:5000, ab13970, Abcam), goat anti-HHIP (1:1000, AF1568, R&D Systems), guinea pig anti-LAMP3 (1:500, 391005, Synaptic Systems), mouse anti-NKX2-1 (1:500, TTF-1-L-CE, Leica Biosystems), goat anti-IL33 (1:500, AF3626, R&D Systems), rabbit anti-CNN1 (1:1000, ab46794, Abcam), rabbit anti-AQP5 (1:2500, ab78486, Abcam) and other:Article Title: KEY RESOURCES TABLE Article Snippet: Article Title: Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes. Article Snippet: Anti-Nsg1 antibody using the same peptide sequences against the N terminus of Nsg1 from aa 7 to 23 (NFA EKG TKQ PLL EDG FD) as in Steiner et al. (2002) was raised in rabbits by Thermo Fisher Scientific and in goats by Everest Biotech with peptide sequences from aa 7 to 20 (1:300 IF; EB11637; RRID: AB_2554545; Yap et al., 2017). |