Journal: EBioMedicine

Article Title: The Notch ligand DNER regulates macrophage IFNγ release in chronic obstructive pulmonary disease

doi: 10.1016/j.ebiom.2019.03.054

Figure Lengend Snippet: DNER is significantly upregulated in lung tissue from COPD patients and localised to pro-inflammatory macrophages. (a) DNER mRNA abundance in lung tissue from healthy subjects ( n = 10) and COPD patients ( n = 15). ** p -value = 0.003, unpaired t -test. Data shown mean values ± SD. (b) Representative immmunofluorescence images from healthy and COPD lung tissue ( n = 2/3) stained to detect localisation of DNER (red), DAPI (blue) and Pro-SPC/CC10/CD31/Acta2/Galectin-3 (green). Scale bar, 10 μm. (c) DNER and TNF mRNA abundance in human monocyte derived macrophages obtained from PBMCs of healthy subjects ( n = 4) and treated with 1 μg/ml LPS in combination with cigarette smoke extract (CSE) 5% for 24 h. Paired t -test, * p = 0.0408. (d) Representative immunofluorescence pictures of DAPI (blue), NOS2 (green) and DNER (red) staining in healthy and COPD lung tissue ( n = 3). Scale bar, 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Human tissue: DNER (1:100 AF3646, R&D Systems), CC10 (1:100 sc-365992), NOS2 (1:20 sc-8310).

Techniques: Staining, Derivative Assay, Immunofluorescence

Journal: PLoS ONE

Article Title: Expansion of Human Mesenchymal Stromal Cells from Fresh Bone Marrow in a 3D Scaffold-Based System under Direct Perfusion

doi: 10.1371/journal.pone.0102359

Figure Lengend Snippet: Top-ten of significant up- and down-regulated expressed genes.

Article Snippet: Number: Hs01039911_m1), EREG (EREG-Applied Biosystems, Ref.

Techniques: