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dna microarray scanner g2505c  (Agilent technologies)


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    Structured Review

    Agilent technologies dna microarray scanner g2505c
    Dna Microarray Scanner G2505c, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+scanner/pm39039509-82-39-38
    Average 90 stars, based on 1 article reviews
    dna microarray scanner g2505c - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis.
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization Conclusions In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. Keywords lncRNA microarray chip, LINC01566, Bioinformatics analysis, CD4 + T cell, Myasthenia gravis and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Activation Assay:

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis.
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization Conclusions In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. Keywords lncRNA microarray chip, LINC01566, Bioinformatics analysis, CD4 + T cell, Myasthenia gravis and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Microarray:

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis.
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization Conclusions In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. Keywords lncRNA microarray chip, LINC01566, Bioinformatics analysis, CD4 + T cell, Myasthenia gravis and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Article Title: Potential Diagnostic Biomarkers of tRNA-Derived Small RNAs in PBMCs for Nonproliferative Diabetic Retinopathy in Patients With Type 2 Diabetes Mellitus.
    Article Snippet: .. The slides were scanned on an Agilent G2505C microarray scanner. .. The acquired array images were analyzed using Agilent Feature Extraction software (version 11.0.1.1).

    Article Title: A network-based trans-omics approach for predicting synergistic drug combinations
    Article Snippet: After hybridization, microarrays were washed for 1 min at room temperature with GE Wash Buffer 1 (Agilent) and 1 min at 37 °C with GE Wash Buffer 2 (Agilent), then dried immediately. .. The slides were scanned immediately after washing using an Agilent DNA Microarray Scanner (G2505C) with a one-color scan setting for 8 × 60 K array slides (Scan Area 61 × 21.6 mm, Scan resolution 3 μm, Dye channel was set to Green and Green PMT was set to 100%). .. The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028282_D_F_20110531) to obtain background subtracted and spatially detrended processed signal intensities.

    Extraction:

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis.
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization Conclusions In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. Keywords lncRNA microarray chip, LINC01566, Bioinformatics analysis, CD4 + T cell, Myasthenia gravis and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Software:

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis.
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization Conclusions In summary, our research revealed the protective roles of LINC01566 in clinical samples and cellular experiments, illustrating the potential roles and mechanism by which FOSL1/LINC01566 negatively regulates CD4 + T-cell activation in MG. Keywords lncRNA microarray chip, LINC01566, Bioinformatics analysis, CD4 + T cell, Myasthenia gravis and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.

    Article Title: FOSL1-mediated LINC01566 negatively regulates CD4 + T-cell activation in myasthenia gravis
    Article Snippet: The labeled cDNAs were hybridized onto the Human LncRNA Array, version 5.0 (8 × 60 K, Arraystar). .. After hybridization and washing, the arrays were scanned using an Agilent G2505C scanner and Agilent Feature Extraction software, version 11.1, to analyze the acquired array images. .. Quantile normalization and subsequent data processing were performed using Agilent GeneSpring GX software, version 12.1.



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    <t>Microarray</t> analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
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    <t>Microarray</t> analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
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    <t>Microarray</t> analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool
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    Flow chart of <t>microarray</t> data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.
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    Image Search Results


    Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: Microarray analysis of transfected HeLa cells with miR-141 overexpressing vector. A Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using a miRNA library from Exiqon. B Microarray analysis of gene expression in HeLa cells transfected with miR-141 overexpression vs. cells transfected with the control vector using oligonucleotides from Ambion. The blue color indicates the sustained gene expression, the red indicates the upregulated genes, and the green indicates the downregulated genes. C The expression of the most relevant genes to cervical cancer indicates the upregulated genes in red columns and downregulated genes in green. Error bars indicate the STD between Exiqon and Ambion data. D The potential binding sites of miR-141 within the 3 − UTR of KLRC1 and coding sequences of KLRC3, CAM3, and that carried out in-silico by miRWalk online tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Transfection, Plasmid Preparation, Expressing, Over Expression, Control, Binding Assay, In Silico

    The prediction information between miR-141 and targeted genes identified by  microarray  analysis using in-silico miRWalk tool

    Journal: BMC Cancer

    Article Title: Regulation of KLRC and Ceacam gene expression by miR-141 supports cell proliferation and metastasis in cervical cancer cells

    doi: 10.1186/s12885-024-12794-6

    Figure Lengend Snippet: The prediction information between miR-141 and targeted genes identified by microarray analysis using in-silico miRWalk tool

    Article Snippet: The microarray experiments were scanned using a DNA microarray laser scanner (DNA Microarray Scanner BA, Agilent Technologies) at 5 µm resolution using Ambion ship and Exiqon ship according to the manufacturer’s instructions.

    Techniques: Microarray, Binding Assay

    Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Journal: bioRxiv

    Article Title: Synovial fluid transcriptome dynamics in osteoarthritis progression: Implications in pathogenesis

    doi: 10.1101/2024.06.24.600143

    Figure Lengend Snippet: Flow chart of microarray data analysis. The agilent single color microarray platform was used having more than 58,000 transcripts and further data processing was done using agilent GeneSpring software followed by pathway analysis using GSEA database. Numbers in parentheses illustrates number of pathways enriched in each category using GSEA database. DEGs-Differentially expressed genes, ANOVA-Analysis of variance, BP-Biological pathway, CC-Cellular component, MF-Molecular function.

    Article Snippet: Following hybridization, the array slides were washed and scanned with an Agilent SureScan High-Resolution DNA Microarray Scanner.

    Techniques: Microarray, Software