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dna microarray chip method  (CapitalBio Corporation)

 
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    CapitalBio Corporation dna microarray chip method
    The layout of the <t>DNA</t> <t>microarray</t> method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.
    Dna Microarray Chip Method, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+microarray+chip+method/dna+microarray+chip/pmc08429459-123-2-1
    Average 90 stars, based on 1 article reviews
    dna microarray chip method - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study"

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-97559-y

    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.
    Figure Legend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Techniques Used: Microarray, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.
    Figure Legend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Techniques Used: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +
    Figure Legend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Techniques Used: Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.
    Figure Legend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Techniques Used: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).
    Figure Legend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Techniques Used: Microarray, Mutagenesis

    Related Articles

    Microarray:

    Article Title: DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection
    Article Snippet: .. The DNA microarray biochip (CapitalBio Company Ltd, Beijing, China) used in the study could identify 17 mycobacterial species, including M. tuberculosis , M. intracellulare , M. avium , M. gordonae , M. kansasii , M. fortuitum , M. scrofulaceum , M. gilvum , M. terrae , M. chelonae/M. abscessus , M. phlei , M. nonchromogenicum , M. marinum/M. ulcerans , M. aurum , M. szulgai/M. malmoense , M. xenopi , and M. smegmatis . ..

    Article Title: Investigating a pulmonary Mycobacterium abscessus infection outbreak among elderly inpatients in the intensive care ward.
    Article Snippet: .. Growing isolates were identified at the species level using a DNA microarray chip from the Mycobacterial Species Identification Array Kit (CapitalBio Technology Inc., Beijing, China) based on previous studies [32–35]. ..

    Article Title: Assessing the utility of the Xpert Mycobacterium tuberculosis /rifampin assay for analysis of bronchoalveolar lavage fluid in patients with suspected pulmonary tuberculosis
    Article Snippet: .. The bacterial strains were identified by the DNA microarray chip analysis (CapitalBio Technology). ..

    Article Title: Identification and characterization of nontuberculous mycobacteria isolated from suspected pulmonary tuberculosis patients in eastern china from 2009 to 2019 using an identification array system
    Article Snippet: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN, Valencia, CA, USA), following the manufacturer's protocol as previously reported. .. DNA microarray chip from Mycobacterial Species Identification Array Kit (CapitalBio Technology Inc., Beijing, China) was performed for NTM species identification as previously reported., , It can identify as many as 17 Mycobacterium species: M. tuberculosis complex, M. chelonae - M. abscessus, M. fortuitum, M. intracellulare, M. avium, M. kansasii, M. gordonae, M. terrae, M. smegmatis, M. szulgai / M. malmoense, M. nonchromogenicum, M. scrofulaceum, M. xenopi, M. aurum, M. marinum / M. ulcerans, M. gilvum , and M. phlei ( ). .. Hybridization patterns of representative strains on the probe array (Mycobacterial Species Identification Array Kit (CapitalBio Technology Inc., Beijing, China)).

    other:

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study
    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.



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    The layout of the <t>DNA</t> <t>microarray</t> method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.
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    Image Search Results


    The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The layout of the DNA microarray method module; each detection panel includes 4 detection modules, which can detect two specimens at the same time. Modules 1 and 3 are used to detect mutations in the rpoB gene, and modules 2 and 4 are used to detect mutations in the katG gene and inhA promoter. QC quality control probe; EC external control probe; BC blank control; NC negative control probe; IC internal control probe; WT wild-type.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Negative Control

    The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: The drug susceptibility test was used as a standard method to evaluate the efficacy of the DNA microarray for detecting RIF and INH resistance and MDR-TB.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Comparison of the diagnostic efficacy of the DNA microarray method when sputum smear grades were ≤ 1 + and ≥ 2 +

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Diagnostic Assay, Microarray

    Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Specimen processing procedure: A total of 5,911 sputum smear-positive specimens were collected. After experimental processing, 4148 specimens that were positive with the DNA microarray method and DST were finally included in the study. NTM, nontuberculous mycobacteria ; DST, drug sensitivity test.

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray

    Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Journal: Scientific Reports

    Article Title: Analysis of the application of a gene chip method for detecting Mycobacterium tuberculosis drug resistance in clinical specimens: a retrospective study

    doi: 10.1038/s41598-021-97559-y

    Figure Lengend Snippet: Pattern diagrams of several common drug-resistant gene mutations detected by the DNA microarray method. The white box is the detection site of the wild-type codon, and the red box is the site of the detected mutant codon. ( a ) rpoB gene Leu511Pro (CTG → CCG); ( b ) rpoB gene Asp516Tyr (GAC → TAC); ( c ) rpoB gene His526Tyr (CAC → TAC); ( d ) rpoB gene Ser531Trp (TCG → TGG); ( e ) rpoB gene Ser531Leu (TCG → TTG); ( f ) katG gene Ser315Thr (AGC → ACC); ( g ) katG gene Ser315Asn (AGC → AAC); ( h ) inhA gene promoter-15 (C → T).

    Article Snippet: The CapitalBio DNA microarray chip method is used to qualitatively detect nucleic acids in samples of Mtb isolates from clinical TB patients.

    Techniques: Microarray, Mutagenesis

    Non-tuberculous species identified from 2013 to 2018 in a tertiary hospital in Beijing, China

    Journal: BMC Microbiology

    Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

    doi: 10.1186/s12866-020-01840-5

    Figure Lengend Snippet: Non-tuberculous species identified from 2013 to 2018 in a tertiary hospital in Beijing, China

    Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

    Techniques:

    Specimen types among which NTM were identified in this study

    Journal: BMC Microbiology

    Article Title: Prevalence of nontuberculous mycobacteria in a tertiary hospital in Beijing, China, January 2013 to December 2018

    doi: 10.1186/s12866-020-01840-5

    Figure Lengend Snippet: Specimen types among which NTM were identified in this study

    Article Snippet: DNA microarray chip method (Mycobacterial Species Identification Array Kit, CapitalBio Technology Inc., Beijing, China) can accurately distinguish between M. avium and M. intracellulae , which have quite similar phenotypes.

    Techniques: