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dna methylation at faim2 promoter sequenom massarray platform  (Sequenom)

 
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    Sequenom dna methylation at faim2 promoter sequenom massarray platform
    Dna Methylation At Faim2 Promoter Sequenom Massarray Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+massarray/dna+methylation+at+faim2+promoter+sequenom+massarray+platform/pmc09263076-9-61-66
    Average 90 stars, based on 1 article reviews
    dna methylation at faim2 promoter sequenom massarray platform - by Bioz Stars, 2026-09
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    DNA Methylation Assay:

    Article Title: Transcriptional and Epigenetic Response to Sedentary Behavior and Physical Activity in Children and Adolescents: A Systematic Review
    Article Snippet: qPCR Transcriptome , Laboratory technique based on polymerase chain reaction (PCR), which is widely used in molecular biology to amplify a specific nucleic acid sequence and obtain millions to billions of copies. This technique is able to quantify gene expression levels. Analysis of transcripts (typically mRNA molecules) in order to assess the gene expression levels. Both microarray and RNA-seq approaches are used. The difference between these methods is that in the array a set of possible genes is defined by the set of probes that are present, while RNA-seq allows detection of known and unknown genes.. .. Wu et al. ( ) , Cross-sectional , Group 1: Children with obesity ( N = 59); Boys + Girls (45.8%); 13.8 ± 3.0 y; Chinese (100%) Group 2: Normal-weight children ( N = 39); Boys + Girls (61.5%); 10.3 ± 1.1 y; Chinese (100%) , SB and PA across 6 months (questionnaire completed by parents or guardians) , Leukocytes , DNA methylation at FAIM2 promoter (Sequenom MassARRAY platform) , Differentially methylation levels at FAIM2 promoter between obese and normal-weight children according to SB and PA levels. Results were not significant after multiple hypothesis testing corrections. .. Lovinsky-Desir et al. ( ) , Cross-sectional , Group 1: Active children ( N = 77); Boys + Girls (45%); 12.2 y (9.2–14.0 y); Hispanic (60%), African American (40%) Group 2: Non-active children ( N = 58); Boys + Girls (55%); 12.7 y (10.5–14.0 y); Hispanic (72%), African American (28%) , PA across 6 days (accelerometer on the non-dominant wrist) , Buccal swabs (squamous epithelial cells) , DNA methylation at FOXP3 promoter (pyrosequencing) and gene expression , Active children had lower FOXP3 promoter methylation compared to Non-active children exposed to high air pollutant black carbon concentrations. No significant association was reported between FOXP3 promoter methylation and gene expression.

    Methylation:

    Article Title: Transcriptional and Epigenetic Response to Sedentary Behavior and Physical Activity in Children and Adolescents: A Systematic Review
    Article Snippet: qPCR Transcriptome , Laboratory technique based on polymerase chain reaction (PCR), which is widely used in molecular biology to amplify a specific nucleic acid sequence and obtain millions to billions of copies. This technique is able to quantify gene expression levels. Analysis of transcripts (typically mRNA molecules) in order to assess the gene expression levels. Both microarray and RNA-seq approaches are used. The difference between these methods is that in the array a set of possible genes is defined by the set of probes that are present, while RNA-seq allows detection of known and unknown genes.. .. Wu et al. ( ) , Cross-sectional , Group 1: Children with obesity ( N = 59); Boys + Girls (45.8%); 13.8 ± 3.0 y; Chinese (100%) Group 2: Normal-weight children ( N = 39); Boys + Girls (61.5%); 10.3 ± 1.1 y; Chinese (100%) , SB and PA across 6 months (questionnaire completed by parents or guardians) , Leukocytes , DNA methylation at FAIM2 promoter (Sequenom MassARRAY platform) , Differentially methylation levels at FAIM2 promoter between obese and normal-weight children according to SB and PA levels. Results were not significant after multiple hypothesis testing corrections. .. Lovinsky-Desir et al. ( ) , Cross-sectional , Group 1: Active children ( N = 77); Boys + Girls (45%); 12.2 y (9.2–14.0 y); Hispanic (60%), African American (40%) Group 2: Non-active children ( N = 58); Boys + Girls (55%); 12.7 y (10.5–14.0 y); Hispanic (72%), African American (28%) , PA across 6 days (accelerometer on the non-dominant wrist) , Buccal swabs (squamous epithelial cells) , DNA methylation at FOXP3 promoter (pyrosequencing) and gene expression , Active children had lower FOXP3 promoter methylation compared to Non-active children exposed to high air pollutant black carbon concentrations. No significant association was reported between FOXP3 promoter methylation and gene expression.



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    Sequenom massarray quantitative dna methylation analysis
    ZNF582-AS1 expression was regulated by <t>DNA</t> <t>methylation</t> in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom <t>MassARRAY</t> quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated
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    ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1

    doi: 10.1186/s13046-021-01889-8

    Figure Lengend Snippet: ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated

    Article Snippet: TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells.

    Techniques: Expressing, DNA Methylation Assay, Sequencing, Comparison, Methylation