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massarray quantitative dna methylation analysis  (Sequenom)

 
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    Structured Review

    Sequenom massarray quantitative dna methylation analysis
    ZNF582-AS1 expression was regulated by <t>DNA</t> <t>methylation</t> in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom <t>MassARRAY</t> quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated
    Massarray Quantitative Dna Methylation Analysis, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+massarray/massarray+quantitative+methylation+analysis/pmc07945252-212-54-53
    Average 90 stars, based on 1 article reviews
    massarray quantitative dna methylation analysis - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1"

    Article Title: Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-021-01889-8

    ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated
    Figure Legend Snippet: ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated

    Techniques Used: Expressing, DNA Methylation Assay, Sequencing, Comparison, Methylation

    Related Articles

    Methylation:

    Article Title: Gender dimorphism in hepatocarcinogenesis—DNA methylation modification regulated X‐chromosome inactivation escape molecule XIST
    Article Snippet: .. Methylation of CpG sites of the Xist first exon region in liver tissues between 10 WT and 10 Tet2 −/− female mice was quantified by Sequenom MassARRAY methylation profiling. ..

    Article Title: Gender dimorphism in hepatocarcinogenesis—DNA methylation modification regulated X‐chromosome inactivation escape molecule XIST
    Article Snippet: .. Methylation of XIST was detected by Sequenom MassARRAY methylation profiling between HCC tissues (T) and adjacent normal liver tissues (L). ..

    Article Title: Exposure to Juvenile Stress Induces Epigenetic Alterations in the GABAergic System in Rats.
    Article Snippet: .. Sequenom Epityper MassARRAY Methylation and Spectra Analysis Samples were desalted and spotted on a 384-alternative patch SpectroCHIP (Se- quenom, San Diego, CA, USA) using the MassARRAY nanodispenser, and spectra were analyzed by the MassARRAY Analyzer Compact matrix-assisted laser desorption/ioniza- tion–time of flight (MALDI-TOF MS) (Sequenom, San Diego, CA, USA). .. Spectra were elaborated by the Epityper 1.2 software (Sequenom, San Diego, CA, USA), which provides methylation values for each CpG unit as a percentage.

    Article Title: DNA Hyper-methylation Associated With Schizophrenia May Lead to Increased Levels of Autoantibodies
    Article Snippet: .. A Sequenom MassARRAY quantitative methylation assay was performed to measure the methylation levels of candidate DMS cg14341177 and cg13978347 in the discovery cohort. .. There are 4 CpG sites on cg14341177, in which the CpG2 site showed the most significant changes in methylation levels and seemed as the leading CpG site.

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    other:

    Article Title: Prediction of treatment response to antipsychotic drugs for precision medicine approach to schizophrenia: randomized trials and multiomics analysis
    Article Snippet: The methylation profiling was validated by two methods including Illumina-sequencing-based BSP and Sequenom MassARRAY® Methylation.

    Article Title: Prediction of treatment response to antipsychotic drugs for precision medicine approach to schizophrenia: randomized trials and multiomics analysis.
    Article Snippet: For participants with methylation data, technical replication (see Technical replication of DNA methylation profiling in Additional file 1) was conducted: 194 participants were randomly chosen for Illumina sequencing-based BSP detection to verify the chip detection site results, and among these 194 participants, 20 were further randomly selected to undergo Sequenom MassARRAY® Methylation validation.

    Expressing:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Western Blot:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Agarose Gel Electrophoresis:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Methylated DNA Immunoprecipitation:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..



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    Sequenom massarray quantitative dna methylation analysis
    ZNF582-AS1 expression was regulated by <t>DNA</t> <t>methylation</t> in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom <t>MassARRAY</t> quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated
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    Image Search Results


    ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1

    doi: 10.1186/s13046-021-01889-8

    Figure Lengend Snippet: ZNF582-AS1 expression was regulated by DNA methylation in ccRCC. a Detection of CpG islands in ZNF582-AS1 promoter and design of MSP primers. The horizontal axis of the curved lines represents the input sequence of ZNF582-AS1, and the vertical axis of the curved lines represents GC percentage. TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells. T refers to Tumor tissue of ccRCC, N refers to Adjacent normal kidney tissue. M = Methylated, U = Unmethylated

    Article Snippet: TSS: Transcription Start Sites. b MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC cell lines. c MSP analysis of ZNF582-AS1 promoter DNA methylation status in ccRCC tissues. d Detection of 38 CpG sites in ZNF582-AS1 promoter. e Quantitative detection of DNA methylation level of 38 CpG sites in ZNF582-AS1 promoter using Sequenom MassARRAY quantitative DNA methylation analysis. f and g Comparison of the DNA methylation levels of 38 CpG sites in ccRCC and adjacent normal renal tissues. h Treatment with 5-aza-dC and TSA demethylated ZNF582-AS1 promoter and increased ZNF582-AS1 expression in OSRC2 and Caki-1 cells.

    Techniques: Expressing, DNA Methylation Assay, Sequencing, Comparison, Methylation