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dapi  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science dapi
    Dapi, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 95/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diluted+dapi+buffer/Dilution+Buffer/pm39657678-337-0-1
    Average 95 stars, based on 173 article reviews
    dapi - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: PRSS23 functions downstream of ZEB1 to promote esophageal squamous cell carcinoma progression by driving M2 polarization of tumor associated macrophages.
    Article Snippet: factors were considered to be associated with the development of EC, such as smoking, alcohol abuse, obesity [5].. EC incudes two main histological subtypes: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) [4].. ESCC most frequently originates in the middle to upper esophagus and accounts for 90% of EC cases in Asia [3, 6, 7].

    Article Title: Divergent stem cell mechanisms govern the primary body axis and appendage regeneration in the axolotl
    Article Snippet: .. Following a wash with PBST to remove the retrieval solution, sections were blocked in 5% donkey serum (Solarbio, ASL050) and incubated overnight at 4°C with primary antibodies diluted in antibody dilution buffer (Genefist, GF1600-1). .. The primary antibodies used in this study included mouse–anti-TUJ1 (R&D, MAB1195), rabbit–anti-CD34 (Abcam, ab81289), rabbit–anti-MEF2C (Santa Cruz Biotechnology, sc-313), rabbit–anti-SOX2 (GeneTex, GTX124477), rabbit–anti-PRRX1 (a gift from E. M. Tanaka’s laboratory), rabbit–anti-SOX10 (Abcam, ab264405), mouse–anti-PAX7 (DSHB), rat–anti-CHERRY (Invitrogen, M11217 ), rabbit–anti-SOX9 (Merck, AB5535), rabbit–anti-Laminin (Sigma-Aldrich, L9393), mouse–anti-MHC [fluorescein isothiocyanate (FITC)–coupled, DSHB], and rabbit–anti–p-SMAD2 (CST, 18338 T).

    Lysis:

    Article Title: LncRNA TLR8-AS1 Restricts HIV-1 Infection and Inflammation in Macrophages by Suppressing Arachidonic Acid Metabolism Through NFAT1
    Article Snippet: .. Cellular protein were extracted using RIPA lysis buffer with a 1:100 dilution of protease inhibitor (Solarbio, China). .. The total protein concentration of the lysates was quantified using a bicinchoninic acid (BCA) assay kit (Beyotime, China).

    Protease Inhibitor:

    Article Title: LncRNA TLR8-AS1 Restricts HIV-1 Infection and Inflammation in Macrophages by Suppressing Arachidonic Acid Metabolism Through NFAT1
    Article Snippet: .. Cellular protein were extracted using RIPA lysis buffer with a 1:100 dilution of protease inhibitor (Solarbio, China). .. The total protein concentration of the lysates was quantified using a bicinchoninic acid (BCA) assay kit (Beyotime, China).

    BIA-KA:

    Article Title: Theabrownin from Pu-erh Tea Restores Intestinal Immunity via Gut Microbiota-Metabolite Network in Immunosuppressed Mice.
    Article Snippet: Flow cytometry antibodies, including CD25-PE (BD Biosciences, San Jose, CA, USA) and CD4-FITC/IL-17A-PE/FOXP3-APC (Thermo Fisher Scientific, Waltham, MA, USA), were used. .. RIPA buffer(high), 5 × Tris-Glycine Running Buffer, 20 × TBST, https://doi.org/10.3390/foods15193381 BCA protein assay kit, antibody dilution buffer, and stripping buffer were purchased from Solarbio (Beijing, China). ..

    Stripping:

    Article Title: Theabrownin from Pu-erh Tea Restores Intestinal Immunity via Gut Microbiota-Metabolite Network in Immunosuppressed Mice.
    Article Snippet: Flow cytometry antibodies, including CD25-PE (BD Biosciences, San Jose, CA, USA) and CD4-FITC/IL-17A-PE/FOXP3-APC (Thermo Fisher Scientific, Waltham, MA, USA), were used. .. RIPA buffer(high), 5 × Tris-Glycine Running Buffer, 20 × TBST, https://doi.org/10.3390/foods15193381 BCA protein assay kit, antibody dilution buffer, and stripping buffer were purchased from Solarbio (Beijing, China). ..

    Western Blot:

    Article Title: NRF2 activation attenuates obesity-induced adipose inflammation by suppressing STING signaling, with depot-specific regulation.
    Article Snippet: Band intensities were quantified using ImageJ software (v1.53c). .. Primary antibodies used for Western blotting were diluted to working concentrations in antibody dilution buffer (Solarbio, Cat. No. A1800). ..

    other:

    Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma.
    Article Snippet: Proteins were transferred to Immobilon PVDF membranes (Millipore).

    Article Title: The aryl hydrocarbon receptor (AHR) drives human leukocyte antigen (HLA)-II expression in human melanoma
    Article Snippet: Proteins were transferred to Immobilon PVDF membranes (Millipore).



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