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Sysmex Corporation diff quik staining method
Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics <t>using</t> <t>Diff-Quik</t> staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
Diff Quik Staining Method, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis"

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

Journal: Clinical and Experimental Otorhinolaryngology

doi: 10.21053/ceo.2025-00170

Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.
Figure Legend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Techniques Used: Diff-Quik, Staining, Standard Deviation

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Staining:

Article Title: Zika virus exacerbates encephalomyelitis by inducing the production of T cell-attracting chemokines in astrocytes
Article Snippet: .. The migrated cells were then fixed and stained with Diff-Quik stainTM (Sysmex, Kobe, Japan). ..

Article Title: Anti-inflammatory effects of 1,8-cineol via NF-κB/COX-2 pathway in BEAS-2B cells and alleviates bronchoconstriction and airway hyperreactivity in ovalbumin sensitized mice
Article Snippet: To distinguish cell types, the pellet was resuspended and the suspended cells were attached to the slide using cytospin (Hanil Science Industrial, Seoul, Republic of Korea) (200× g at 4°C for 10 min). .. Cells on dried slides were fixed and stained with Diff-Quik ® reagent (Sysmex Co., Kobe, Japan). .. The levels of the type 2 cytokines interleukin-4, -13, and -17 were determined via a commercial enzyme-linked immunosorbent assay (ELISA, RayBiothech Life, Incorporation, Georgia Norcross) and detected at 450 nm using a plate reader of ELISA (Bio-Rad Laboratories).

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Article Snippet: .. Migrated cells on the lower surface were stained using Diff-Quik stain solution (cat no. 38721; AR TIC LE IN PR ES S Sysmex Corporation, Kobe, Japan) at room temperature. .. After drying, stained cells were imaged under a light microscope (Olympus IX51; Olympus Corporation, Tokyo, Japan), and cell counts were obtained from five randomly selected fields per chamber.

Article Title: Pulmonary responses to polyacrylic acid with and without carboxyl groups following intratracheal instillation in rats.
Article Snippet: 1 Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 2 Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 3 Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 4 Shared-Use Research Center, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 5 Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan 6 Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan Abstract Polyacrylic acid (PAA) is a widely used organic polymer synthesized from acrylic acid monomers.. Its viscosity and water absorbency can be modified through cross-linking, making it suitable for various applications, including shampoos, food products, and pharmaceuticals.. However, several reports have suggested that PAA has high potential for pulmonary injury, especially if exposed during product manufacturing processes.

Diff-Quik:

Article Title: Zika virus exacerbates encephalomyelitis by inducing the production of T cell-attracting chemokines in astrocytes
Article Snippet: .. The migrated cells were then fixed and stained with Diff-Quik stainTM (Sysmex, Kobe, Japan). ..

Article Title: Anti-inflammatory effects of 1,8-cineol via NF-κB/COX-2 pathway in BEAS-2B cells and alleviates bronchoconstriction and airway hyperreactivity in ovalbumin sensitized mice
Article Snippet: To distinguish cell types, the pellet was resuspended and the suspended cells were attached to the slide using cytospin (Hanil Science Industrial, Seoul, Republic of Korea) (200× g at 4°C for 10 min). .. Cells on dried slides were fixed and stained with Diff-Quik ® reagent (Sysmex Co., Kobe, Japan). .. The levels of the type 2 cytokines interleukin-4, -13, and -17 were determined via a commercial enzyme-linked immunosorbent assay (ELISA, RayBiothech Life, Incorporation, Georgia Norcross) and detected at 450 nm using a plate reader of ELISA (Bio-Rad Laboratories).

Article Title: Overcoming resistance in CD44-overexpressing myeloma through combination therapy with ATRA, bortezomib, and NK cells.
Article Snippet: .. Migrated cells on the lower surface were stained using Diff-Quik stain solution (cat no. 38721; AR TIC LE IN PR ES S Sysmex Corporation, Kobe, Japan) at room temperature. .. After drying, stained cells were imaged under a light microscope (Olympus IX51; Olympus Corporation, Tokyo, Japan), and cell counts were obtained from five randomly selected fields per chamber.

Article Title: Pulmonary responses to polyacrylic acid with and without carboxyl groups following intratracheal instillation in rats.
Article Snippet: 1 Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 2 Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 3 Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 4 Shared-Use Research Center, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 5 Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan 6 Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan Abstract Polyacrylic acid (PAA) is a widely used organic polymer synthesized from acrylic acid monomers.. Its viscosity and water absorbency can be modified through cross-linking, making it suitable for various applications, including shampoos, food products, and pharmaceuticals.. However, several reports have suggested that PAA has high potential for pulmonary injury, especially if exposed during product manufacturing processes.

other:

Article Title: HERV-R (ERV3-1) Env gene knockout reduces invasion, migration, and tumor growth in DLD1 colon cancer cells
Article Snippet: After culturing for 18 hours at 37°C, cells that had migrated to the lower chamber were stained with Diff-Quik (Sysmex Corporation, 38721) and counted.

Article Title: Cigarette Smoke Induces the Pro-inflammatory Response of Lung Macrophages via Activation of the IL-33/ST2-Dependent Pathway
Article Snippet: Recovered cells were centrifuged onto glass slides at 1,000 rpm for 5 min using a cytospin (Shandon CytoSpin III; Thermo Shandon, Runcorn, UK) and stained with Diff-Quik (Sysmex Co., Kobe, Japan).

Article Title: Aged garlic extract suppressed macrophage-mediated inflammation in acute respiratory distress syndrome.
Article Snippet: Total cell counts were determined using Türk’s solution, and differential cell counts were performed on cytospin preparations stained with Diff-Quik (Sysmex International Reagents, Kobe, Japan, Cat. No. 16920).

Article Title: Bone Marrow Stromal Cell Antigen 2 Promotes Esophageal Squamous Cell Carcinoma Progression by Mediating the Cell-Cell Interaction between Cancer Cells and Cancer-Associated Fibroblasts
Article Snippet: After 48 hours, the cells that had migrated to the underside of the membrane were stained with Diff-Quik (Sysmex, Kobe, Japan), and the number of migrated cells was quantified across five randomly selected fields.

Light Microscopy:

Article Title: Pulmonary responses to polyacrylic acid with and without carboxyl groups following intratracheal instillation in rats.
Article Snippet: 1 Department of Respiratory Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 2 Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 3 Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 4 Shared-Use Research Center, School of Medicine, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8555, Japan 5 Department of Chemistry and Biochemistry, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan 6 Research Institute of Science for Safety and Sustainability, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan Abstract Polyacrylic acid (PAA) is a widely used organic polymer synthesized from acrylic acid monomers.. Its viscosity and water absorbency can be modified through cross-linking, making it suitable for various applications, including shampoos, food products, and pharmaceuticals.. However, several reports have suggested that PAA has high potential for pulmonary injury, especially if exposed during product manufacturing processes.



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Image Search Results


Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Journal: Clinical and Experimental Otorhinolaryngology

Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

doi: 10.21053/ceo.2025-00170

Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

Techniques: Diff-Quik, Staining, Standard Deviation