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diff-quik stained method  (Anhui Anke)

 
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    Anhui Anke diff-quik stained method
    Diff Quik Stained Method, supplied by Anhui Anke, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diff-quik+staining+method/diff+quick+staining/pmc12101436-74-13-17
    Average 90 stars, based on 1 article reviews
    diff-quik stained method - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Comparison of sperm parameters and DNA fragmentation index between infertile men with infection and vaccines of COVID-19.
    Article Snippet: Sperm morphology was performed after Diff-Quick staining (Ankebio, Chongqing, China).

    Article Title: Correlation of the sperm DNA fragmentation index with semen parameters and its impact on fresh embryo transfer outcomes—a retrospective study
    Article Snippet: Fresh liquefied semen smears were air dried and analyzed for sperm morphology via Diff-Quik stained method (AnHui, Ankebio, Moscow, Russia).

    Article Title: Comparison of sperm parameters and DNA fragmentation index between infertile men with infection and vaccines of COVID-19
    Article Snippet: Sperm morphology was performed after Diff-Quick staining (Ankebio, Chongqing, China).

    Article Title: Association of sexually transmitted infection with semen quality in men from couples with primary and secondary infertility
    Article Snippet: Sperm morphology was evaluated through Diff-Quick staining (Ankebio, Hefei, China) at 100× magnification under a light microscope (UB100i, UOP, Chongqing, China).

    Article Title: Association of paternal MTHFR polymorphisms (C677T) with clinical outcomes in ICSI treatment
    Article Snippet: Sperm morphology was evaluated through Diff-Quick staining (Ankebio, Hefei, China) at ×100 magnification under a light microscope (UB100i, UOP, Chongqing, China).

    Staining:

    Article Title: Association between semen collection time and semen parameters: an observational study
    Article Snippet: Sperm concentration, progressive motility, and total motility were evaluated by computer-assisted sperm analysis (CASA), including phase contrast microscopy (CX43, Olympus Corporation, Tokyo, Japan) and the SAS-II system (SAS Medical, Beijing, China). .. Sperm morphology was evaluated with a semen slide stained with Diff-Quick (Ankebio, Hefei, China) and observed in a light microscope (UB100i, UOP, Chongqing, China). .. Leukocytes were stained by peroxidase using benzidine (Ankebio).

    Article Title: Effects of COVID-19 or vaccines for SARS-COV-2 on sperm parameters: A systematic review and meta-analysis.
    Article Snippet: This systematic review and meta-analysis assessed the effects of SARS-Cov-2 and mRNA vaccines on male reproduction.. We conducted a comprehensive search using terms such as "COVID-19," "vaccine," and "sperm parameters" on various electronic databases, including MEDLINE (PubMed), BVS (LILACS and others), Embase (Elsevier), and Cochrane Library.. We included observational studies, including cohort, case-control, and crosssectional studies.

    Article Title: SARS-CoV-2 Vaccination Improves Semen Quality in Men Recovered From COVID-19: A Retrospective Cohort Study
    Article Snippet: .. According to the 2010 World Health Organization (WHO) laboratory manual for the examination and processing of human semen, sperm morphology was evaluated with a semen slide stained with Diff-Quick (Ankebio, Hefei, China). .. Leukocytes were stained using the Peroxidase Leukocyte Kit (Ankebio).

    Diff-Quik:

    Article Title: Association between semen collection time and semen parameters: an observational study
    Article Snippet: Sperm concentration, progressive motility, and total motility were evaluated by computer-assisted sperm analysis (CASA), including phase contrast microscopy (CX43, Olympus Corporation, Tokyo, Japan) and the SAS-II system (SAS Medical, Beijing, China). .. Sperm morphology was evaluated with a semen slide stained with Diff-Quick (Ankebio, Hefei, China) and observed in a light microscope (UB100i, UOP, Chongqing, China). .. Leukocytes were stained by peroxidase using benzidine (Ankebio).

    Article Title: SARS-CoV-2 Vaccination Improves Semen Quality in Men Recovered From COVID-19: A Retrospective Cohort Study
    Article Snippet: .. According to the 2010 World Health Organization (WHO) laboratory manual for the examination and processing of human semen, sperm morphology was evaluated with a semen slide stained with Diff-Quick (Ankebio, Hefei, China). .. Leukocytes were stained using the Peroxidase Leukocyte Kit (Ankebio).

    Light Microscopy:

    Article Title: Association between semen collection time and semen parameters: an observational study
    Article Snippet: Sperm concentration, progressive motility, and total motility were evaluated by computer-assisted sperm analysis (CASA), including phase contrast microscopy (CX43, Olympus Corporation, Tokyo, Japan) and the SAS-II system (SAS Medical, Beijing, China). .. Sperm morphology was evaluated with a semen slide stained with Diff-Quick (Ankebio, Hefei, China) and observed in a light microscope (UB100i, UOP, Chongqing, China). .. Leukocytes were stained by peroxidase using benzidine (Ankebio).

    Article Title: Effects of COVID-19 or vaccines for SARS-COV-2 on sperm parameters: A systematic review and meta-analysis.
    Article Snippet: This systematic review and meta-analysis assessed the effects of SARS-Cov-2 and mRNA vaccines on male reproduction.. We conducted a comprehensive search using terms such as "COVID-19," "vaccine," and "sperm parameters" on various electronic databases, including MEDLINE (PubMed), BVS (LILACS and others), Embase (Elsevier), and Cochrane Library.. We included observational studies, including cohort, case-control, and crosssectional studies.



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    Image Search Results


    Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

    Journal: Clinical and Experimental Otorhinolaryngology

    Article Title: Synergistic Exacerbation of Allergic Inflammation by Combined Exposure to Air-Pollutants in a Murine Model of Allergic Rhinitis

    doi: 10.21053/ceo.2025-00170

    Figure Lengend Snippet: Inflammatory cell counts in nasal and bronchoalveolar lavage fluids (NALF and BALF) following single or combined exposure to PM2.5, Zn, and formaldehyde in a murine model of allergic rhinitis. (A) Total and differential cell counts, including eosinophils, neutrophils, lymphocytes, and monocytes, were measured in NALF from non-sensitized mice after single intranasal exposure to PM2.5, Zn, or formaldehyde. (B) Total and differential cell counts were assessed in BALF from the same non-sensitized mice following identical single exposures. (C) In ovalbumin (OVA)-sensitized mice, total and differential cell counts in NALF were evaluated after additional intranasal exposure to PM2.5 alone or in combination with Zn or formaldehyde. (D) Total and differential cell counts in BALF were measured in the same OVA-sensitized mice after combined exposures. Inflammatory cells were identified based on morphological characteristics using Diff-Quik staining. Values are presented as mean±standard deviation. NS, not significant. Statistical analysis was conducted with one-way analysis of variance followed by Tukey’s post hoc test. * P <0.05, ** P <0.01, *** P <0.001 vs. OVA or OVA+PM2.5, as indicated.

    Article Snippet: Slides were stained using the Diff-Quik staining method (Sysmex).

    Techniques: Diff-Quik, Staining, Standard Deviation