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synthesis n a mit synthesis n a gelred biotium 41003 deposited data rna sequence data  (Biotium)


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    Biotium synthesis n a mit synthesis n a gelred biotium 41003 deposited data rna sequence data
    Synthesis N A Mit Synthesis N A Gelred Biotium 41003 Deposited Data Rna Sequence Data, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/GelRed+Nucleic+Acid+Gel+Stain/pmc07419720__mmc1-79-171-177
    Average 97 stars, based on 3838 article reviews
    synthesis n a mit synthesis n a gelred biotium 41003 deposited data rna sequence data - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Construct:

    Article Title: Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants.
    Article Snippet: .. Our RNA ID constructs were run on a 0.8−1% (w/v) agarose gel (agarose for molecular biology, Sigma, catalogue number A9539), 1× TBE (0.089 M Tris−Borate, 0.002 M EDTA, pH 8.3; Sigma, catalogue number SRE0062) in an ice bath, for 1.5 h at 70 V. The gel was poststained in 3× GelRed solution (Biotium, catalogue number 41001) for 10 min on a horizontal shaker. ..

    Agarose Gel Electrophoresis:

    Article Title: Modular RNA:DNA Nanostructures Enable Nanopore Profiling of rRNA Processing and rRNA Variants.
    Article Snippet: .. Our RNA ID constructs were run on a 0.8−1% (w/v) agarose gel (agarose for molecular biology, Sigma, catalogue number A9539), 1× TBE (0.089 M Tris−Borate, 0.002 M EDTA, pH 8.3; Sigma, catalogue number SRE0062) in an ice bath, for 1.5 h at 70 V. The gel was poststained in 3× GelRed solution (Biotium, catalogue number 41001) for 10 min on a horizontal shaker. ..

    Article Title: Swarming in the storm: Collembola aggregations on sub-Antarctic Marion Island
    Article Snippet: The individual springtails were then homogenized using a pestle, and each PCR tube was placed into a MultiGene OptiMax thermal cycler with the following conditions: initial denaturation at 96○C for 5 min, 40 cycles of 30 s at 96○C for denaturation, 30 s at 48○C for annealing and 50 s at 72○C for extension, followed by a final extension step at 72○C for 10 min. All samples underwent postPCR clean-up using the Macherey-Nagel NucleoSpin® Gel and PCRClean-upKit following themanufacturer’s recommendations. .. Successful amplifications were confirmed using 2% agarose gel electrophoresis with 0.5X TBE buffer and the GelRed Nucleic Acid Gel Stain (Biotium). .. PCR products were sequenced with the forward primer on a 3730XLDNAAnalyzer (Applied Biosystems).

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: .. Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity.

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Staining:

    Article Title: Swarming in the storm: Collembola aggregations on sub-Antarctic Marion Island
    Article Snippet: The individual springtails were then homogenized using a pestle, and each PCR tube was placed into a MultiGene OptiMax thermal cycler with the following conditions: initial denaturation at 96○C for 5 min, 40 cycles of 30 s at 96○C for denaturation, 30 s at 48○C for annealing and 50 s at 72○C for extension, followed by a final extension step at 72○C for 10 min. All samples underwent postPCR clean-up using the Macherey-Nagel NucleoSpin® Gel and PCRClean-upKit following themanufacturer’s recommendations. .. Successful amplifications were confirmed using 2% agarose gel electrophoresis with 0.5X TBE buffer and the GelRed Nucleic Acid Gel Stain (Biotium). .. PCR products were sequenced with the forward primer on a 3730XLDNAAnalyzer (Applied Biosystems).

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: .. Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity.

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: .. Samples were visualized by GelRedTM Nucleic Acid Stain (cat 41003, Biotium, Fremont, CA, USA) using the Azure 300 imaging system (cat AZI30001, Azure Biosystems, Dublin, CA, USA). .. The image quantification was performed by densitometric analysis using Image Lab software v6.1 (cat 12012931, Bio-Rad Laboratories, Hercules, CA, USA).

    Fluorescence:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Microscopy:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Single Cell Gel Electrophoresis:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Software:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Amplification:

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: .. Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity.

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA). .. Droplet digital PCR reactions were performed on all samples that passed these quality control steps, using the Bio-Rad QX200 system.

    Electrophoresis:

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: .. Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity.

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA). .. Droplet digital PCR reactions were performed on all samples that passed these quality control steps, using the Bio-Rad QX200 system.

    Imaging:

    Article Title: The impact of Triticum aestivum L. cultivation management on genomic polymorphism of Harpalus rufipes and Silpha obscura assessed by RAPD markers.
    Article Snippet: .. Amplified products were separated by electrophoresis on a 1.5% agarose gel, stained with GelRedTM Nucleic Acid Stain (Biotium), and visualized using a G-Box imaging system (SYNGENE). .. The banding patterns were interpreted using GeneTools software (SYNGENE), which enables automatic detection of distinct amplicons based on signal intensity.

    Article Title: Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.
    Article Snippet: .. Samples were visualized by GelRedTM Nucleic Acid Stain (cat 41003, Biotium, Fremont, CA, USA) using the Azure 300 imaging system (cat AZI30001, Azure Biosystems, Dublin, CA, USA). .. The image quantification was performed by densitometric analysis using Image Lab software v6.1 (cat 12012931, Bio-Rad Laboratories, Hercules, CA, USA).

    Isolation:

    Article Title: Fanconi Anemia in Mexican Patients: Molecular Spectrum and Clinical Manifestations in a Case Series.
    Article Snippet: Genomic DNA was isolated from PB using QIAamp® DNA Blood Mini kit (Qiagen GmbH, Germantown, MD, USA) according to the manufacturer’s instructions. .. Genomic isolated DNA integrity was analyzed by electrophoresis on 1% agarose gel stained with GelRed (Biotium, Fremont, CA, USA; Cat. No. 41003). .. DNA concentration was determined using spectrophotometry, with the absorbance (A) at 260 nm (A260) measured; genomic DNA “purity” was assessed by reading the A230 and A280, resulting in values of ~1.8 and 2.1 for A260/A280 and A260/A230 ratio, according to the results obtained with the NanoDropTM One (NanoDrop Technologies; Thermo Fisher Scientific, Inc., Grand Island, NY, USA).

    Marker:

    Article Title: Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cells
    Article Snippet: .. A 4% agarose gel was prepared in 1x TBE buffer, stained with GelRed (Biotium, 41003), and electrophoresis with a GeneRuler 50 bp DNA Ladder (Thermo Fisher Scientific, SM0371) as a size marker. .. Electrophoresis was performed at 120 V for 1–2 h. Gel images were captured using the FUSION FX VILBER system.

    Polymerase Chain Reaction:

    Article Title: Comparison of cell isolates within anatomical regions of the ovine umbilical cord for improved isolation of putative mesenchymal stem cells.
    Article Snippet: PCR reactions of 25 μl were prepared containing final concentrations of 1 x β-2-microglobulin qPCR primer-probe assay (Table 1), 0.2 mM each dNTPs (Thermo Fisher Scientific, Waltham, USA, Invitrogen Cat No. 10297018), 1.5 mM MgCl2 and 1.5 units taq DNA polymerase (New England Biolabs, Ipswich, USA, Cat No. M0320S) along with 1 ng of the synthesized cDNA. .. Amplification was performed using a T100TM Thermal Cycler (Bio-Rad, Hercules, USA) with initial denaturation at 94 ◦C for 1 min; 34 cycles of 94 ◦C for 30 s, 55 ◦C for 30 s, 72 ◦C for 30 s; followed by a final extension step at 72 ◦C for 7 min. PCR products (10 μl) along with 2.5 μl of 1 Kb Plus DNA Ladder at each end (Thermo Fisher Scientific, Waltham, USA, Cat. No. 10787018) were resolved by electrophoresis on 3% agarose gels, prepared by dissolving AxygenTM Agarose LE (Thermo Fisher Scientific, Waltham, USA, Cat. No. 14223081) and 0.5 μl Gel Red dye (Biotium, Fremont, USA, Cat. No. 41003) in 1 x Tris-Boric acid-EDTA buffer (Merck KGaA, Darmstadt, Germany, Sigma Aldrich Cat. No's T1503-1 KG, B0252-1 KG, 10425S) run at 100 V for 45 min and imaged for the expected 119 bp amplicon using a Bio-Rad Chemidoc MP (Bio-Rad, Hercules, USA). .. Droplet digital PCR reactions were performed on all samples that passed these quality control steps, using the Bio-Rad QX200 system.



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