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deposited data microarray data  (Illumina Inc)


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    Illumina Inc deposited data microarray data
    Deposited Data Microarray Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/pm31775048-185-125-123
    Average 99 stars, based on 11451 article reviews
    deposited data microarray data - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Virological and epidemiological investigations of a severe hand foot and mouth disease outbreak, Vietnam, 2023
    Article Snippet: The cDNA was then converted to double-stranded DNA using exo-Klenow (Invitrogen), and subsequently pre-amplified using Platinum PCR supermix (Invitrogen) and FR20RV primer. .. PCR product was then purified and subjected to library preparation using Illumina COVIDseq reagents (Illumina, San Diego, CA, USA), and was finally sequenced using MiSeq reagent kits (Illumina) in an Illumina MiSeq platform (Illumina). ..

    Purification:

    Article Title: Virological and epidemiological investigations of a severe hand foot and mouth disease outbreak, Vietnam, 2023
    Article Snippet: The cDNA was then converted to double-stranded DNA using exo-Klenow (Invitrogen), and subsequently pre-amplified using Platinum PCR supermix (Invitrogen) and FR20RV primer. .. PCR product was then purified and subjected to library preparation using Illumina COVIDseq reagents (Illumina, San Diego, CA, USA), and was finally sequenced using MiSeq reagent kits (Illumina) in an Illumina MiSeq platform (Illumina). ..

    Sequencing:

    Article Title: Simplifying Macroinvertebrate Biodiversity Assessment: A Standardised Metabarcoding Method for Invertebrate Samples With High Volumes of Debris
    Article Snippet: PCR conditions were as follows: 94°C for 5 min followed by 12 cycles of 94°C for 30 s, 55°C for 30 s, 72°C for 30 s, then 1 cycle of 72°C for 5 min. Amplicons were pooled in equal amounts, and the library was gel purified using a PureLink Quick Gel Extraction and PCR Purification Combo Kit (Invitrogen, Waltham, MA, USA) and for the final library the concentration was estimated with a Qubit Fluorometer using the dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA). .. An Illumina MiSeq run using a 600‐cycle flow cell MiSeq sequencing kit V3 (300 bp × 2) (Illumina, San Diego, CA, USA) was performed by the Australian Genome Research Facility Ltd. (AGRF, Melbourne, Australia) to generate sequence data for each sample. ..

    Article Title: The stone-degrading metabolic potential of the urinary microbiome: Sexual dimorphism and dysbiosis in urolithiasis
    Article Snippet: Libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and Qubit dsDNA HS Assay Kit (Thermo Scientific). .. Paired-end sequencing (2 × 300 bp) was performed on the Illumina MiSeq platform using the MiSeq Reagent Kit v3. ..

    Article Title: Mining Impacts on a Threatened and Burnt Swamp Ecosystem: Responses of the Microbial Soil Community
    Article Snippet: Amplified products were purified and reamplified in a second indexing PCR, adding a unique Illumina Nextera XT index to each sample. .. Paired‐end amplicon sequencing was performed on the Illumina MiSeq platform using MiSeq Reagents kit v3 2 × 250 bp Nano. .. Similar procedures were applied to sequence the hypervariable region V9 of the 18S rRNA gene; instead, 1391F (5′‐GTACACACCGCCCGTC‐3′) and EukB (5′‐TGATCCTTCTGCAGGTTCACCTAC‐3′) primers were used, while afterward the paired‐end amplicon sequencing was performed on the Illumina MiSeq platform using 2 × 250 bp.

    Article Title: MtvS1 and MtvS2 Interact with RNA polymerase to regulate the Francisella type V-A CRISPR-Cas system.
    Article Snippet: Final DNA concentrations and lengths were measured with a Qubit 4 fluorometer (Invitrogen) using the Qubit dsDNA High Sensitivity Assay Kit and the Tapestation 4200 bioanalyzer (Agilent) with the Agilent D500 ScreenTape Assay (Agilent). .. The DNA libraries for the mtvS1 nonsense mutant were diluted to 12 pM, mixed with 2.5% 20 pM PhiX and subjected to single read sequencing with a 150-cycle MiSeq Reagent Kit v3 (Illumina) on the Illumina MiSeq platform (firmware v4.0). ..

    Article Title: Identification of SARS-CoV-2-specific cytotoxic lymphocytes in cerebrospinal fluid of a patient with cerebral demyelination Post mRNA vaccination
    Article Snippet: The generated libraries were measured for their DNA concentration by Qubit and assessed for their size by an Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, CA, USA). .. TCR sequencing was performed on an Illumina MiSeq sequencer using the 600-cycle MiSeq reagent kit v3 (Illumina) with pair-end, 2 × 300 base pair reads. ..

    Amplification:

    Article Title: Mining Impacts on a Threatened and Burnt Swamp Ecosystem: Responses of the Microbial Soil Community
    Article Snippet: Amplified products were purified and reamplified in a second indexing PCR, adding a unique Illumina Nextera XT index to each sample. .. Paired‐end amplicon sequencing was performed on the Illumina MiSeq platform using MiSeq Reagents kit v3 2 × 250 bp Nano. .. Similar procedures were applied to sequence the hypervariable region V9 of the 18S rRNA gene; instead, 1391F (5′‐GTACACACCGCCCGTC‐3′) and EukB (5′‐TGATCCTTCTGCAGGTTCACCTAC‐3′) primers were used, while afterward the paired‐end amplicon sequencing was performed on the Illumina MiSeq platform using 2 × 250 bp.

    Mutagenesis:

    Article Title: MtvS1 and MtvS2 Interact with RNA polymerase to regulate the Francisella type V-A CRISPR-Cas system.
    Article Snippet: Final DNA concentrations and lengths were measured with a Qubit 4 fluorometer (Invitrogen) using the Qubit dsDNA High Sensitivity Assay Kit and the Tapestation 4200 bioanalyzer (Agilent) with the Agilent D500 ScreenTape Assay (Agilent). .. The DNA libraries for the mtvS1 nonsense mutant were diluted to 12 pM, mixed with 2.5% 20 pM PhiX and subjected to single read sequencing with a 150-cycle MiSeq Reagent Kit v3 (Illumina) on the Illumina MiSeq platform (firmware v4.0). ..



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