densitometry (Bio-Rad)
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Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 35689 article reviews
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other:Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals Article Snippet: After washing and incubating with an appropriate Software:Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad). .. The signal intensity of bands were quantified utilizing Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats. Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using Article Title: Single-cell RNA sequencing identifies a proinflammatory S100a8/a9 + macrophage subset contributing to acute kidney injury. Article Snippet: 1 Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China 2 Kidney and Blood Purification Laboratory of Shanghai, Shanghai, China 3 Shanghai Medical Center of Kidney, Shanghai, China 4 Kidney and Dialysis Institute of Shanghai, Shanghai, China 5 Hemodialysis Quality Control Center of Shanghai, Shanghai, China Abstract Objective Macrophages represent a heterogeneous cell population in the kidney.. However, macrophage subsets and their role in endotoxemia-induced acute kidney injury (AKI) remain elucidated.. Material and methods Single-cell RNA sequencing was performed to identify macrophage subpopulations in mouse kidney following lipopolysaccharide (LPS) challenge. Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy. Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes. Article Title: OGT regulates RNA-binding protein nucleocytoplasmic partitioning, but this fails in amyotrophic lateral sclerosis. Article Snippet: All membranes were imaged using a ChemiDoc MP imaging system (Bio-Rad). .. Band intensities were analyzed using Alternative Splicing:Article Title: Alternative splicing of a species- and skeletal muscle-specific microexon diversifies the menu of clathrin heavy chains in mice and rats. Article Snippet: PCR products were resolved via 6% polyacrylamide gel electrophoresis using TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA pH 8.5) for 3–4 h at 150–160 V. Polyacrylamide gels were stained with 0.4 μg/mL ethidium bromide for 10 min. All gels were imaged under ultraviolet illumination in a ChemiDoc XRS+ Imaging System (Bio-Rad). .. Alternative splicing was quantified by densitometry using the Incubation:Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy. Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes. Automatic:Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy. Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes. Fluorescence:Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy. Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes. Imaging:Article Title: Targeting activated hepatic stellate cells through ferroptosis: phenethyl isothiocyanate attenuates CCl 4 -induced liver fibrosis by regulating iron metabolism, redox homeostasis, and autophagy. Article Snippet: Phenethyl isothiocyanate (PEITC), a dietary compound from cruciferous vegetables, is known for its chemopreventive activity, but its effects on liver fibrosis remain largely unexplored.. In this study, we show that PEITC administration attenuated carbon tetrachloride (CCl4)-induced hepatic injury and fibrosis in mice, as evidenced by reduced collagen deposition, hydroxyproline content and fibrotic marker expression, and improved liver function.. PEITC inhibited cell survival and fibrogenic phenotypes in transforming growth factor β (TGF-β)-stimulated LX-2 human hepatic stellate cells (HSCs) while exhibiting minimal cytotoxicity towards normal hepatocytes. Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and |
