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densitometry quantification  (Bio-Rad)


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    Bio-Rad densitometry quantification
    Densitometry Quantification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+software/Image+Lab+Software/pm41397959-607-0-7
    Average 99 stars, based on 35737 article reviews
    densitometry quantification - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific).

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad).

    Software:

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution. .. Band intensities were quantified using Image Lab software (Bio-Rad). ..

    Article Title: Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann Cells.
    Article Snippet: Membranes were probed with the following antibodies: anti-NF1 #sc-376886 (1:200) by Santa Cruz Biotechnology (Dallas, TX, USA), anti-PARP #10872500 (1:1000) by Roche, anti-NRF2 #PA58804 (1:1000) by Life Technologies, anti-TRX #EPR6110 (1:1000) by Abcam (Cambridge, UK), anti-SOD1 #TA50495 (1:1000) by OriGene Technologies, (Rockville, MD, USA), anti-cleaved caspase-3 (Asp175) #9661 (1:1000) by Cell Signaling Technology (Danvers, MA, USA) and anti-GAPDH-HRP #MA5-15738-HRP (1:5000) by Life Technologies. .. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Image Lab software 5.0 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Staining:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Imaging:

    Article Title: CCAN forms the spherical shell-like structure in interphase kinetochores.
    Article Snippet: The supernatant was then boiled at 96°C for 5 min. For SDS–polyacrylamide gel electrophoresis (PAGE) analysis, 25 pmol of each protein sample and 10 μl of the supernatant were loaded as the input and pull- down samples, respectively, on a 10–20% SuperSep Ace gel (Wako, catalog no. 191- 15031). .. After visualizing the protein bands by CBB staining, gel images were acquired using the ChemiDoc Touch MP imaging system (Bio- Rad) and analyzed using the Image Lab software v6.1.0. .. For quantitative analysis, the band intensity of pulled down CENP- N was normalized by that of GST–Mini- CENP- C. Data are shown as mean ± SD from three technical replicates and generated using GraphPad Prism v9.5.1.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories).

    Incubation:

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis
    Article Snippet: .. Following incubation with HRP‐conjugated goat anti‐rabbit IgG and goat anti‐mouse IgG secondary antibodies (both 1:5000), protein bands were analyzed using Image Lab 6.0 software (BioRad, USA). ..



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    Image Search Results


    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control

    ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Western Blot, Control

    Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control