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    Structured Review

    Bio-Rad scanning densitometry
    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
    Scanning Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+scanning/Computer/pmc11505644-44-1-5
    Average 96 stars, based on 1209 article reviews
    scanning densitometry - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut"

    Article Title: Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut

    Journal: Cells

    doi: 10.3390/cells13201698

    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
    Figure Legend Snippet: BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Techniques Used: Inhibition, Expressing, Transfection, Control, Real-time Polymerase Chain Reaction, Western Blot

    Related Articles

    Electrophoresis:

    Article Title: Molecular screening and characterization of Leptospira spp. in cattle and rodent populations in Morobe and East New Britain Provinces, Papua New Guinea
    Article Snippet: .. Following electrophoresis and staining visualization, gels were documented using a BIORAD Gel DocTM EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) connected to a computer system for image capture and analysis. .. Positive PCR products were prepared for Sanger sequencing according to Macrogen Inc. (Singapore) specifications: PCR products at concentrations > 80 ng/μl in volumes > 10 μl, with primers supplied at 5 pmol/μl (5 μl per reaction).

    Staining:

    Article Title: Molecular screening and characterization of Leptospira spp. in cattle and rodent populations in Morobe and East New Britain Provinces, Papua New Guinea
    Article Snippet: .. Following electrophoresis and staining visualization, gels were documented using a BIORAD Gel DocTM EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) connected to a computer system for image capture and analysis. .. Positive PCR products were prepared for Sanger sequencing according to Macrogen Inc. (Singapore) specifications: PCR products at concentrations > 80 ng/μl in volumes > 10 μl, with primers supplied at 5 pmol/μl (5 μl per reaction).

    Fluorescence:

    Article Title: SuperSelective PCR primers for determining cis/trans configurations of mutations within the same gene
    Article Snippet: .. The point at which this transition occurred (the “threshold cycle,” abbreviated “Ct”) was automatically calculated from the fluorescence intensity data at each thermal cycle by the Bio-Rad CFX Maestro computer program. ..

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    Imaging:

    Article Title: Hippocampal Ring Finger Protein 10-dependent signaling supports cognitive flexibility
    Article Snippet: Finally, membranes were scanned with a Chemidoc (Biorad Universal Hood III) using Image Lab software (Bio-Rad). .. Bands were quantified with computer-assisted imaging (Image Lab, Biorad). ..

    Polymerase Chain Reaction:

    Article Title: Seamless and Highly Efficient Site‐directed Mutagenesis for Protein, RNA, and Plasmid Engineering
    Article Snippet: .. H 2 O, autoclaved Nanopure 2× Q5 hot‐start master mix (New England Biolabs, cat. no. M0494S) 2× Platinum SuperFi II PCR master mix (Thermo Fisher Scientific, cat. no. 12368010) DpnI, 20 U/μl (New England Biolabs, cat. no. R0176) A desktop or laptop computer, e.g., a 14‐inch Mac Pro laptop computer SnapGene software package (version 8.0.3) installed on the computer P2, P20, P200 and P1000 pipettes Autoclaved pipette tips PCR tubes (Diamed, cat. no. DIATEC420‐1378) Bio‐Rad PCR T100 Thermal Cycler ..

    Software:

    Article Title: Seamless and Highly Efficient Site‐directed Mutagenesis for Protein, RNA, and Plasmid Engineering
    Article Snippet: .. H 2 O, autoclaved Nanopure 2× Q5 hot‐start master mix (New England Biolabs, cat. no. M0494S) 2× Platinum SuperFi II PCR master mix (Thermo Fisher Scientific, cat. no. 12368010) DpnI, 20 U/μl (New England Biolabs, cat. no. R0176) A desktop or laptop computer, e.g., a 14‐inch Mac Pro laptop computer SnapGene software package (version 8.0.3) installed on the computer P2, P20, P200 and P1000 pipettes Autoclaved pipette tips PCR tubes (Diamed, cat. no. DIATEC420‐1378) Bio‐Rad PCR T100 Thermal Cycler ..

    Article Title: Construction and characterization of the first Arcobacter butzleri - Escherichia coli shuttle vector
    Article Snippet: All digestion products were electrophoresed in 2% agarose (Bio-Rad, Hercules, USA) gels in 1× TBE (Tris/Borate/EDTA) buffer with a 1:20,000 GelRed (Biotium, Fremont, USA) stock solution (10,000 x in water). .. Visualization was achieved using a UV transilluminator (ChemiDoc XRS system, Bio-Rad, Hercules, USA) with an analyst computer program (Quantity One XRD software, Bio-Rad, Hercules, USA). ..

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    Article Title: Construction and characterization of the first Arcobacter butzleri - Escherichia coli shuttle vector.
    Article Snippet: All digestion products were electrophoresed in 2% agarose (Bio-Rad, Hercules, USA) gels in 1× TBE (Tris/Borate/EDTA) buffer with a 1:20,000 GelRed (Biotium, Fremont, USA) stock solution (10,000 x in water). .. Visualization was achieved using a UV transilluminator (ChemiDoc XRS system, Bio-Rad, Hercules, USA) with an analyst computer program (Quantity One XRD software, Bio-Rad, Hercules, USA). ..

    Article Title: Lysine-11 ubiquitination drives type-I/III interferon induction by cGAS-STING and Toll-like receptors 3 and 4.
    Article Snippet: .. Software and code Policy information about availability of computer code Data collection QPCR data were obtained from Bio-Rad CFX Opus 96. .. Imaging data was collected by using S360, Hamamatsu Slidescanner with Autoloader, 40X objective.

    Transferring:

    Article Title: Seamless and Highly Efficient Site‐directed Mutagenesis for Protein, RNA, and Plasmid Engineering
    Article Snippet: .. H 2 O, autoclaved Nanopure 2× Q5 hot‐start master mix (New England Biolabs, cat. no. M0494S) 2× Platinum SuperFi II PCR master mix (Thermo Fisher Scientific, cat. no. 12368010) DpnI, 20 U/μl (New England Biolabs, cat. no. R0176) A desktop or laptop computer, e.g., a 14‐inch Mac Pro laptop computer SnapGene software package (version 8.0.3) installed on the computer P2, P20, P200 and P1000 pipettes Autoclaved pipette tips PCR tubes (Diamed, cat. no. DIATEC420‐1378) Bio‐Rad PCR T100 Thermal Cycler ..

    Western Blot:

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    Immunohistochemistry:

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    Flow Cytometry:

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    Immunofluorescence:

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.

    High Performance Liquid Chromatography:

    Article Title: Targeted inhibition of FBXL2 confers susceptibility of HER2-negative breast cancer to trastuzumab deruxtecan.
    Article Snippet: Trastuzumab deruxtecan (T-DXd), an anti-HER2–drug conjugate, has transformed treatment for HER2-expressing solid tumors.. However, heterogeneous intratumoral HER2 expression, particularly high densities of HER2-immunohistochemistry score 0 (HER2-IHC 0) cells, limits its clinical efficacy.. Here, we discovered that targeted inhibition of F-box protein FBXL2 elevates HER2 expression on the plasma membrane of HER2-IHC 0 triple-negative breast cancer (TNBC) cells, thereby sensitizing them to T-DXd.



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    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by <t>densitometry</t> scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.
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    Image Search Results


    BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Journal: Cells

    Article Title: Bromodomain-Containing 4 Is a Positive Regulator of Interleukin-34 Production in the Gut

    doi: 10.3390/cells13201698

    Figure Lengend Snippet: BRD4 inhibition reduces the expression of IL-34. ( A ). IBD LPMCs were transfected with either a scrambled control oligonucleotide (NC AS) or BRD4 AS for 24 h and BRD4 (black) and IL-34 (grey) RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. Data indicate mean ± SEM of 4 independent experiments. ( B ). IBD LPMCs were transfected with either NC AS or BRD4 AS for 48 h and BRD4, IL-34, and β-actin were analyzed by Western blotting. One of 4 independent experiments is shown. The right panels show the quantitative analysis of the BRD4/β-actin ratio and IL-34/β-actin ratio in protein extracts as measured by densitometry scanning of Western blots. Values are expressed in arbitrary units (a.u.) and indicate the mean ± SEM of all experiments. ( C ). IBD LPMCs were treated with JQ1 (200 nM) or vehicle (DMSO) for 24 h, and IL-34 RNA transcripts were analyzed by real-time PCR. Levels were normalized to β-actin. The data indicate mean ± SEM of 4 independent experiments. ( D ). Mice received either regular drinking water (CTR) or dextran sulfate sodium (DSS) and were killed on day 8. BRD4 and IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of 6 CTR mice and 9 DSS-treated mice. ( E ). Mice receiving DSS were intraperitoneally given JQ1 (DSS+JQ1) or vehicle (DMSO) on days 3 and 6 and then killed on day 8. IL-34 mRNA expression was evaluated in colonic tissue by real-time PCR and levels were normalized to β-actin. The data indicate mean ± SEM of all samples.

    Article Snippet: Computer-assisted scanning densitometry (Image-Lab 5.2.1, Bio-Rad Laboratories, Milan, Italy) was used to analyze the intensity of the immunoreactive bands.

    Techniques: Inhibition, Expressing, Transfection, Control, Real-time Polymerase Chain Reaction, Western Blot