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raw deep-sequencing data  (Biotechnology Information)

 
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    Biotechnology Information raw deep-sequencing data
    Raw Deep Sequencing Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deep-sequencing+raw+data/deep+sequencing+data/pm37890669-454-0-11
    Average 90 stars, based on 1 article reviews
    raw deep-sequencing data - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Identification of small non-coding RNAs as sperm quality biomarkers for in vitro fertilization
    Article Snippet: .. The deep sequencing data have been deposited in the National Center for Biotechnology Information Expression Omnibus (GEO) ( http://www.ncbi.nlm.nih.gov/geo/ ) under accession number GSE110190. ..

    Article Title: Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
    Article Snippet: .. The deep sequencing data have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE95218. ..

    Article Title: Identification of substrates of the small RNA methyltransferase Hen1 in mouse spermatogonial stem cells and analysis of its methyl-transfer domain
    Article Snippet: .. The deep sequencing data have been deposited in the National Center for Biotechnology Information under accession number GSE97595 . ..

    Article Title: Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
    Article Snippet: .. The deep-sequencing data from this study have been submitted to the National Center for Biotechnology Information Sequence Read Archive under accession number SRP307854 . ..

    Article Title: Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain.
    Article Snippet: .. The deep-sequencing data from this study have been submitted to the National Center for Biotechnology Information Sequence Read Archive under accession number SRP307854. ..

    Expressing:

    Article Title: Identification of small non-coding RNAs as sperm quality biomarkers for in vitro fertilization
    Article Snippet: .. The deep sequencing data have been deposited in the National Center for Biotechnology Information Expression Omnibus (GEO) ( http://www.ncbi.nlm.nih.gov/geo/ ) under accession number GSE110190. ..

    Gene Expression:

    Article Title: Single-cell CAS-seq reveals a class of short PIWI-interacting RNAs in human oocytes
    Article Snippet: .. The deep sequencing data have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus under accession number GSE95218. ..

    Article Title: The piRNA pathway is essential for generating functional oocytes in golden hamsters.
    Article Snippet: 1State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.. 2State Key Laboratory of Reproductive Medicine, Jiangsu Laboratory Animal Center, Jiangsu Animal Experimental Center of Medicine and Pharmacy, Jiangsu Key Laboratory of Xenotransplantation, Department of Cell Biology, Animal Core facility, Key Laboratory of Model Animal, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, China.. 3Clinical Center of Reproductive Medicine, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

    other:

    Article Title: TMEM241 is a UDP-N-acetylglucosamine transporter required for M6P modification of NPC2 and cholesterol transport.
    Article Snippet: Raw deep-sequencing data have been deposited in the National Center for Biotechnology Information Sequence Read Archive (PRJNA1015588).

    Article Title: Genetic disruption of oncogenic Kras sensitizes lung cancer cells to Fas receptor-mediated apoptosis
    Article Snippet: Data deposition: The deep-sequencing data reported in this paper have been deposited in the National Center for Biotechnology Information BioProject database (accession no. PRJNA356881 ).



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses

    doi: 10.1016/j.celrep.2019.11.070

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Illumina deep-sequencing raw data , This manuscript , https://data.mendeley.com/datasets/77x6pyfk79/1 https://data.mendeley.com/datasets/ryr859j6pr/1.

    Techniques: Virus, Recombinant, Purification, One Step RT-PCR, Reverse Transcription, Gel Extraction, Library Quantification, DNA Library Preparation, Sample Prep, Software

    Approach for combined transcriptomics and viral sequencing of single influenza virus-infected cells that express IFN. (A) IFN reporter A549 cells are infected with a mix of wild-type and synonymously barcoded viruses. IFN + cells are enriched by MACS and pooled with nonenriched cells and uninfected canine cells that serve as controls for multiplets and mRNA leakage. (B) The mRNAs from individual cells are converted to cDNAs tagged with cell-specific barcodes. (C) Cellular transcriptomes are quantified using standard single-cell 3′-end Illumina sequencing, and (D) viral genes are enriched by influenza virus-specific PCR and fully sequenced by PacBio (in this schematic, only the cell labeled by the red barcode is infected and has viral transcripts that are sequenced by PacBio). (E) The result is a matrix giving the expression of each gene in each cell, as well as the full sequences of the viral genes in infected cells.

    Journal: Journal of Virology

    Article Title: Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity

    doi: 10.1128/JVI.00500-19

    Figure Lengend Snippet: Approach for combined transcriptomics and viral sequencing of single influenza virus-infected cells that express IFN. (A) IFN reporter A549 cells are infected with a mix of wild-type and synonymously barcoded viruses. IFN + cells are enriched by MACS and pooled with nonenriched cells and uninfected canine cells that serve as controls for multiplets and mRNA leakage. (B) The mRNAs from individual cells are converted to cDNAs tagged with cell-specific barcodes. (C) Cellular transcriptomes are quantified using standard single-cell 3′-end Illumina sequencing, and (D) viral genes are enriched by influenza virus-specific PCR and fully sequenced by PacBio (in this schematic, only the cell labeled by the red barcode is infected and has viral transcripts that are sequenced by PacBio). (E) The result is a matrix giving the expression of each gene in each cell, as well as the full sequences of the viral genes in infected cells.

    Article Snippet: Briefly, the raw deep-sequencing data from the Illumina 3′-end sequencing were processed using the 10x Genomics software package cellranger (version 2.2.0).

    Techniques: Sequencing, Infection, Labeling, Expressing