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Abbott Laboratories dapi ii antifade solution
Dapi Ii Antifade Solution, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Related Articles

Fluorescence:

Article Title: Genetic alterations and expression of PTEN and its relationship with cancer stem cell markers to investigate pathogenesis and to evaluate prognosis in hepatocellular carcinoma.
Article Snippet: © Author(s) (or their employer(s)) 2019.. No commercial re-use.. See rights and permissions.

Article Title: Chromosome analysis in embryos from young patients with previous parity.
Article Snippet: .. Following hybridization, the slides were counterstained in antifade solution (Antifade II; Abbott Laboratories) and observed by fluorescence microscopy (Olympus BX51 and Genetics GSL-10 with Olympus BX61; Tokyo, Japan) equipped with the following filter sets: triple-band filters (aqua, orange, green) and single-band pass filters (red, green, gold, aqua, blue). .. Images were captured at ×600 magnification using Jai M2 Camera (Stemmer Imaging, Surrey, UK) associated with Cytovision software + Z –stack (Leica Biosystem, Nunningen, Switzerland).

Article Title: Five chromosome segregation in polar bodies and the corresponding oocyte.
Article Snippet: For a comprehe 1472-6483/$ see front mat doi:10.1016/j.rbmo.2011.11 M Cristina Magli has co-ordinated the laboratories of Italian Society for the Study of Reproductive Medicine (SISMeR), in Bologna from 1995 to date and she is responsible for the Unit of Research and Development.. Between 2008 and 2011, she was co-ordinator of the European Society of Human Reproduction and Embryology (ESHRE) Special Interest Group on Embryology and she is currently a member of the ESHRE Executive Committee.. Her current research interests include studies of gamete and embryo morphology and development, especially in relation to their chromosomal status. nsive picture of the meiotic process and to follow up its products, five chromosomes were tested by fluorescent in-situ hybridization in both polar bodies (PB) and corresponding 145 oocytes.

Article Title: Long survival of PD-L1-positive mediastinal sarcomatoid carcinoma after immunotherapy and anti-angiogenic target therapy: A case report
Article Snippet: .. Slides were counterstained with DAPI II antifade solution (Abbott Molecular) and examined under a fluorescence microscope equipped with single-bandpass filters for DAPI (excitation 358–405 nm), Spectrum Green (excitation ~495 nm) and Spectrum Orange (excitation ~546 nm), using a ×100 oil-immersion objective. ..

Article Title: Assessing the utility of confirmatory studies following identification of large-scale genomic imbalances by microarray.
Article Snippet: INTRODUCTION The identification of genomic imbalances that result in the loss or gain of clinically significant genetic material assists clinicians in the diagnosis, prognosis, and medical management of affected individuals.. The tools available to assess genomic imbalances have advanced greatly in the past few decades, evolving from lower-resolution techniques such as conventional karyotyping and fluorescence in situ hybridization (FISH) to much higherresolution techniques such as single-nucleotide polymorphism microarray.1 Because of its detection ability and clinical utility, the guidelines set forth by the American College of Medical Genetics and Genomics currently recommend microarray as a first-tier test for patients with intellectual disability, autism, and multiple congenital anomalies.2,3 Historically, FISH has been considered a gold standard for confirmation of copy-number changes identified by microarray.4 However, high-density microarray platforms are identifying an increasing number of aberrations that cannot be confirmed using FISH, for a variety of reasons: (i) the aberration is smaller than the detection limits of the FISH technology, (ii) the aberration is located in a region of the genome for which a clone is not readily available, or (iii) the aberration is not a result of a genomic imbalance (i.e., a region of homozygosity).. With the increasing familiarity of microarray analysis, the use of FISH or another secondary technology for confirmation of suspicious or pathogenic genomic imbalances identified by microarray has declined in some laboratories.

Microscopy:

Article Title: Genetic alterations and expression of PTEN and its relationship with cancer stem cell markers to investigate pathogenesis and to evaluate prognosis in hepatocellular carcinoma.
Article Snippet: © Author(s) (or their employer(s)) 2019.. No commercial re-use.. See rights and permissions.

Article Title: Chromosome analysis in embryos from young patients with previous parity.
Article Snippet: .. Following hybridization, the slides were counterstained in antifade solution (Antifade II; Abbott Laboratories) and observed by fluorescence microscopy (Olympus BX51 and Genetics GSL-10 with Olympus BX61; Tokyo, Japan) equipped with the following filter sets: triple-band filters (aqua, orange, green) and single-band pass filters (red, green, gold, aqua, blue). .. Images were captured at ×600 magnification using Jai M2 Camera (Stemmer Imaging, Surrey, UK) associated with Cytovision software + Z –stack (Leica Biosystem, Nunningen, Switzerland).

Article Title: Five chromosome segregation in polar bodies and the corresponding oocyte.
Article Snippet: For a comprehe 1472-6483/$ see front mat doi:10.1016/j.rbmo.2011.11 M Cristina Magli has co-ordinated the laboratories of Italian Society for the Study of Reproductive Medicine (SISMeR), in Bologna from 1995 to date and she is responsible for the Unit of Research and Development.. Between 2008 and 2011, she was co-ordinator of the European Society of Human Reproduction and Embryology (ESHRE) Special Interest Group on Embryology and she is currently a member of the ESHRE Executive Committee.. Her current research interests include studies of gamete and embryo morphology and development, especially in relation to their chromosomal status. nsive picture of the meiotic process and to follow up its products, five chromosomes were tested by fluorescent in-situ hybridization in both polar bodies (PB) and corresponding 145 oocytes.

Article Title: Long survival of PD-L1-positive mediastinal sarcomatoid carcinoma after immunotherapy and anti-angiogenic target therapy: A case report
Article Snippet: .. Slides were counterstained with DAPI II antifade solution (Abbott Molecular) and examined under a fluorescence microscope equipped with single-bandpass filters for DAPI (excitation 358–405 nm), Spectrum Green (excitation ~495 nm) and Spectrum Orange (excitation ~546 nm), using a ×100 oil-immersion objective. ..

Hybridization:

Article Title: Chromosome analysis in embryos from young patients with previous parity.
Article Snippet: .. Following hybridization, the slides were counterstained in antifade solution (Antifade II; Abbott Laboratories) and observed by fluorescence microscopy (Olympus BX51 and Genetics GSL-10 with Olympus BX61; Tokyo, Japan) equipped with the following filter sets: triple-band filters (aqua, orange, green) and single-band pass filters (red, green, gold, aqua, blue). .. Images were captured at ×600 magnification using Jai M2 Camera (Stemmer Imaging, Surrey, UK) associated with Cytovision software + Z –stack (Leica Biosystem, Nunningen, Switzerland).

Article Title: Five chromosome segregation in polar bodies and the corresponding oocyte.
Article Snippet: For a comprehe 1472-6483/$ see front mat doi:10.1016/j.rbmo.2011.11 M Cristina Magli has co-ordinated the laboratories of Italian Society for the Study of Reproductive Medicine (SISMeR), in Bologna from 1995 to date and she is responsible for the Unit of Research and Development.. Between 2008 and 2011, she was co-ordinator of the European Society of Human Reproduction and Embryology (ESHRE) Special Interest Group on Embryology and she is currently a member of the ESHRE Executive Committee.. Her current research interests include studies of gamete and embryo morphology and development, especially in relation to their chromosomal status. nsive picture of the meiotic process and to follow up its products, five chromosomes were tested by fluorescent in-situ hybridization in both polar bodies (PB) and corresponding 145 oocytes.

Concentration Assay:

Article Title: Characterization of the OmyY1 Region on the Rainbow Trout Y Chromosome
Article Snippet: Antibodies to Spectrum Orange (Molecular Probes) were used to amplify the signal. .. Slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI) at a concentration of 125 ng DAPI in 1 mL antifade solution (Abbott Molecular). .. Images were captured with a Jai camera and analyzed with Cytovision Genus (Applied Imaging, Inc.) software.



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(A) Porcine small intestinal crypts were embedded in Matrigel domes and cultured to establish small intestinal organoids. (B) Mature organoids were treated with 20 μM lipopolysaccharide (LPS) for 24 h to induce inflammation. (C) Treated organoids were analyzed using qRT-PCR, <t>DAPI</t> and PI staining, and immunofluorescence to assess gene expression, cell viability, and protein expression.
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(A) Porcine small intestinal crypts were embedded in Matrigel domes and cultured to establish small intestinal organoids. (B) Mature organoids were treated with 20 μM lipopolysaccharide (LPS) for 24 h to induce inflammation. (C) Treated organoids were analyzed using qRT-PCR, DAPI and PI staining, and immunofluorescence to assess gene expression, cell viability, and protein expression.

Journal: PLOS One

Article Title: Lipopolysaccharide-induced alterations in barrier integrity and inflammatory signaling in porcine small intestinal organoids

doi: 10.1371/journal.pone.0359086

Figure Lengend Snippet: (A) Porcine small intestinal crypts were embedded in Matrigel domes and cultured to establish small intestinal organoids. (B) Mature organoids were treated with 20 μM lipopolysaccharide (LPS) for 24 h to induce inflammation. (C) Treated organoids were analyzed using qRT-PCR, DAPI and PI staining, and immunofluorescence to assess gene expression, cell viability, and protein expression.

Article Snippet: The stained organoids were washed with PBS, mounted on glass slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA, USA), and nuclear staining was performed simultaneously.

Techniques: Cell Culture, Organoids, Quantitative RT-PCR, Staining, Immunofluorescence, Gene Expression, Expressing

(A) Phase-contrast images of pSIOs treated with LPS (0, 20, and 30 μM) showing structural disruption, core darkening, and fragmentation at higher concentrations compared with controls. Scale bars: 200 μm. (B) DAPI (blue) and PI (red) staining of pSIOs treated with LPS (5, 10, 20, and 30 μM) showing a dose-dependent increase in apoptosis. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each LPS concentration in each biological replicate. Scale bars: 200 μm. (C) Representative immunofluorescence images of E-cadherin (green) and DAPI (blue) staining of pSIOs showing reduced and discontinuous E-cadherin expression in LPS-treated organoids compared with controls. Enlarged views of the yellow boxed regions are shown. Low-magnification (200 μm) images and enlarged views (50 μm) are shown in 3C. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. (D) Relative gene expression levels of CDH1 and TJP1 in pSIOs treated with LPS (20 μM) compared with controls (0 μM), as determined by qRT-PCR. LPS treatment significantly decreased the expression of CDH1 and TJP1 , indicating disruption of epithelial barrier integrity. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. *** p < 0.001.

Journal: PLOS One

Article Title: Lipopolysaccharide-induced alterations in barrier integrity and inflammatory signaling in porcine small intestinal organoids

doi: 10.1371/journal.pone.0359086

Figure Lengend Snippet: (A) Phase-contrast images of pSIOs treated with LPS (0, 20, and 30 μM) showing structural disruption, core darkening, and fragmentation at higher concentrations compared with controls. Scale bars: 200 μm. (B) DAPI (blue) and PI (red) staining of pSIOs treated with LPS (5, 10, 20, and 30 μM) showing a dose-dependent increase in apoptosis. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each LPS concentration in each biological replicate. Scale bars: 200 μm. (C) Representative immunofluorescence images of E-cadherin (green) and DAPI (blue) staining of pSIOs showing reduced and discontinuous E-cadherin expression in LPS-treated organoids compared with controls. Enlarged views of the yellow boxed regions are shown. Low-magnification (200 μm) images and enlarged views (50 μm) are shown in 3C. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. (D) Relative gene expression levels of CDH1 and TJP1 in pSIOs treated with LPS (20 μM) compared with controls (0 μM), as determined by qRT-PCR. LPS treatment significantly decreased the expression of CDH1 and TJP1 , indicating disruption of epithelial barrier integrity. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. *** p < 0.001.

Article Snippet: The stained organoids were washed with PBS, mounted on glass slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA, USA), and nuclear staining was performed simultaneously.

Techniques: Phase Contrast, Disruption, Staining, Organoids, Concentration Assay, Immunofluorescence, Expressing, Gene Expression, Quantitative RT-PCR

(A) Immunofluorescence staining of TLR4 in pSIOs after LPS (20 μM) treatment. Nuclei were stained with DAPI (blue), and TLR4 expression is shown in green. Representative images suggest higher TLR4 immunofluorescence in the LPS-treated group than in the control group. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. Scale bar = 100 μm. (B) qRT-PCR analysis of pro- and anti-inflammatory cytokines in pSIOs after LPS (20 μM) exposure. Pro-inflammatory cytokines TNF-α , IL-1β , and IL-8 were significantly upregulated in the LPS-treated group compared with the control group. Anti-inflammatory cytokines IL-4 and IL-10 also showed significantly increased expression, whereas TGF-β expression was increased but did not reach statistical significance following LPS stimulation. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. * p < 0.05, ** p < 0.01.

Journal: PLOS One

Article Title: Lipopolysaccharide-induced alterations in barrier integrity and inflammatory signaling in porcine small intestinal organoids

doi: 10.1371/journal.pone.0359086

Figure Lengend Snippet: (A) Immunofluorescence staining of TLR4 in pSIOs after LPS (20 μM) treatment. Nuclei were stained with DAPI (blue), and TLR4 expression is shown in green. Representative images suggest higher TLR4 immunofluorescence in the LPS-treated group than in the control group. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. Scale bar = 100 μm. (B) qRT-PCR analysis of pro- and anti-inflammatory cytokines in pSIOs after LPS (20 μM) exposure. Pro-inflammatory cytokines TNF-α , IL-1β , and IL-8 were significantly upregulated in the LPS-treated group compared with the control group. Anti-inflammatory cytokines IL-4 and IL-10 also showed significantly increased expression, whereas TGF-β expression was increased but did not reach statistical significance following LPS stimulation. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. * p < 0.05, ** p < 0.01.

Article Snippet: The stained organoids were washed with PBS, mounted on glass slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA, USA), and nuclear staining was performed simultaneously.

Techniques: Immunofluorescence, Staining, Expressing, Control, Organoids, Quantitative RT-PCR, Analysis

(A) Immunofluorescence of pSIOs stained with DAPI (blue) to visualize nuclei and immunolabeled for SGLT1 (green), PEPT1 (green), GLUT2 (green), TGR5 (red), and GLP-1 (green). Representative untreated (upper rows) and LPS-treated (lower rows) organoids are shown. Untreated organoids exhibited distinct localization of SGLT1, PEPT1, GLUT2, and TGR5 along the epithelial membrane with strong expression and cytoplasmic GLP-1 expression in scattered enteroendocrine-like cells within the epithelial layer. LPS-treated organoids exhibited reduced expression intensity, irregular protein distribution, and partial loss of membrane localization. Enlarged views of the yellow boxed regions further highlight differences in expression patterns and subcellular localization between untreated and LPS-treated organoids. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. Scale bars: 50 μm. (B) qRT-PCR analysis showing significant alterations in nutrient transporter and enteroendocrine-related gene expression following LPS treatment. Decreased SLC5A1 (SGLT1) and SLC15A1 (PEPT1) expression and increased SLC2A2 expression were observed after LPS exposure. Among enteroendocrine-related genes, GPBAR1 expression was markedly decreased, whereas GCG (the precursor gene of GLP-1) expression was significantly increased after LPS exposure.Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. ** p < 0.01, *** p < 0.001.

Journal: PLOS One

Article Title: Lipopolysaccharide-induced alterations in barrier integrity and inflammatory signaling in porcine small intestinal organoids

doi: 10.1371/journal.pone.0359086

Figure Lengend Snippet: (A) Immunofluorescence of pSIOs stained with DAPI (blue) to visualize nuclei and immunolabeled for SGLT1 (green), PEPT1 (green), GLUT2 (green), TGR5 (red), and GLP-1 (green). Representative untreated (upper rows) and LPS-treated (lower rows) organoids are shown. Untreated organoids exhibited distinct localization of SGLT1, PEPT1, GLUT2, and TGR5 along the epithelial membrane with strong expression and cytoplasmic GLP-1 expression in scattered enteroendocrine-like cells within the epithelial layer. LPS-treated organoids exhibited reduced expression intensity, irregular protein distribution, and partial loss of membrane localization. Enlarged views of the yellow boxed regions further highlight differences in expression patterns and subcellular localization between untreated and LPS-treated organoids. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate. Scale bars: 50 μm. (B) qRT-PCR analysis showing significant alterations in nutrient transporter and enteroendocrine-related gene expression following LPS treatment. Decreased SLC5A1 (SGLT1) and SLC15A1 (PEPT1) expression and increased SLC2A2 expression were observed after LPS exposure. Among enteroendocrine-related genes, GPBAR1 expression was markedly decreased, whereas GCG (the precursor gene of GLP-1) expression was significantly increased after LPS exposure.Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. ** p < 0.01, *** p < 0.001.

Article Snippet: The stained organoids were washed with PBS, mounted on glass slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, Burlingame, CA, USA), and nuclear staining was performed simultaneously.

Techniques: Immunofluorescence, Staining, Immunolabeling, Organoids, Membrane, Expressing, Quantitative RT-PCR, Analysis, Gene Expression