Review



dapi  (Dojindo Labs)


Bioz Verified Symbol Dojindo Labs is a verified supplier
Bioz Manufacturer Symbol Dojindo Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Dojindo Labs dapi
    Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/Cellstain+DAPI+solution/pmc13636658-94-53-54
    Average 96 stars, based on 489 article reviews
    dapi - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: Primary and secondary antibodies used were rabbit polyclonal anti-phospho-S6K (1:500, #9209, Cell Signaling Technology), rabbit monoclonal anti-phospho-Akt (1:500, #4060, Cell Signaling Technology), rabbit monoclonal anti-Akt (1:500, #4691, Cell Signaling Technology), goat polyclonal anti-GAPDH (1:500, IMG-3073, Imgenex), HRP-linked anti-rabbit IgG (1:2,000, #7074, Cell Signaling Technology), and HRP-linked anti-goat IgG (1:2,000, ab97110, Abcam).

    Staining:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Correlation-Based Morphometric Analysis Reveals Structural Remodeling of Platelet-Bound Circulating Tumor Cells in Urothelial Carcinoma.
    Article Snippet: Circulating tumor cells (CTCs) mediate hematogenous metastasis and are key targets of liquid biopsy.. Although platelet−tumor cell interactions enhance survival and dissemination, systematic studies of CTCs remain limited because of their rarity and heterogeneity.. We report a single-cell approach for evaluating platelet-bound CTCs.

    Incubation:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Blocking Assay:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Cell-Signaling:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Modification:

    Article Title: 4-Phenylbutyric acid activates an NF-κB-Egr-1 axis to control myoblast proliferation and ECM gene expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack Roche Cat #: 5892791001 DAPI (4′,6-diamidino-2-phenylindole) Solution Dojindo Cat #: D523 Dimethyl sulfoxide (DMSO) Nacalai tesque Cat #: 13408-64 (−)-DHMEQ MedChemExpress Cat #: HY-14645 Dulbecco’s Modified Eagle Medium (DMEM), high glucose Nacalai tesque Cat #: 08459-64 Ethanol FUJIFILM Wako Pure Chemical Corporation Cat #: 054-07225 Fetal bovine serum (FBS) (Lot. .. 0001641439) Nichirei Biosciences Cat #: 173012-500ML Glycine Nacalai tesque Cat #: 17109-35 Hanks’ Balanced Salt Solution/HBSS Nacalai Tesque Cat #: 17461-05 HCl FUJIFILM Wako Pure Chemical Corporation Cat #: 084-05425 Horse serum (HS) GIBCO Cat #:26050088 Immobilon Western Chemiluminescent HRP Substrate Cytiva Cat #: RPN2235 Lipofectamine RNAiMAX transfection reagent Invitrogen Cat #: 13778075 Hilymax Dojindo Cat #: H357 M-PER Mammalian Protein Extraction Reagent Thermo Fisher Cat #: 78501 Matrigel Corning Cat #: 354234 NaCl FUJIFILM Wako Pure Chemical Corporation Cat #: 191-01665 Paraformaldehyde (37% PFA, for crosslinking) FUJIFILM Wako Pure Chemical Corporation Cat #: 063-04815 Paraformaldehyde (4% PFA) FUJIFILM Wako Pure Chemical Corporation Cat #:163-20145 Penicillin-Streptomycin Mixed Solution Nacalai tesque Cat #: 26253-84 Phosphatase Inhibitor Cocktail Solution II(×100) FUJIFILM Wako Pure Chemical Corporation Cat #: 160-24371 Phosphate-buffered saline (PBS, pH 7.4) Nacalai tesque Cat #: 14249-24 ProLong Glass Antifade Mountant Thermo Fisher Cat #: P36965 Protein A Mag Sepharose Cytiva Cat #: 28944006 PicoLab Rodent Diet 20 LabDiet Cat #: 5053 Red Blood Cell (RBC) Lysis Buffer/RCK buffer BioLegend Cat #: 420301 Sample Buffer Solution with Reducing Reagent(6×) for SDS-PAGE pH6.8 Nacalai tesque Cat #: 09499-14 Sodium Dodecyl Sulfate FUJIFILM Wako Pure Chemical Corporation Cat #: 199-07141 Tauroursodeoxycholic Acid (TUDCA) Selleck Biotech Cat #: S3654 Triton X-100 Sigma-Aldrich Cat #: 9002-93-1 Trypsin-EDTA (0.25%) Nacalai tesque Cat #: 35554-64 Tunicamycin Cayman Chemical Cat #: 11445 Tween 20 Sigma-Aldrich Cat #: P9416-100ML Silencer Select Mouse Hdac5 siRNA (Assay ID s67423) Invitrogen Cat #: 4390771 Silencer Select Mouse Egr-1 siRNA (Assay ID s65378) Invitrogen Cat #: 4390771 Silencer Select Negative Control No. 1 siRNA Invitrogen Cat #: 4390843 Critical commercial assays Agilent 4200 TapeStation D1000 assay Agilent Cat #: D1000 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat #: G3582 ISOSPIN Cell & Tissue RNA kit Nippon Gene Cat #: 314-08211 JC-1 MitoMP Detection Kit Dojindo Cat #: MT09 Maxwell RSC Simply RNA Cell Kit Promega Cat #: AS1390 (Continued on next page) iScience 29, 117620, October 16, 2026 17



    Similar Products

    98
    Vector Laboratories vectashield antifade mounting media
    Vectashield Antifade Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pmc13657169-395-13-17
    Average 98 stars, based on 1 article reviews
    vectashield antifade mounting media - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    99
    Thermo Fisher 4 6 diamidino 2 phenylindole
    4 6 Diamidino 2 Phenylindole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/4'%2C6'-Diamidino-2-phenylindole+dihydrochloride%2C+98%25/pmc13241941-122-5-7
    Average 99 stars, based on 1 article reviews
    4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    95
    Novus Biologicals dapi
    Dapi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI+Solution/pmc13657219-28-0-2
    Average 95 stars, based on 1 article reviews
    dapi - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    99
    Beyotime 4 6 diamino 2 phenylindole dapi

    4 6 Diamino 2 Phenylindole Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI/pmc13657243-22-0-3
    Average 99 stars, based on 1 article reviews
    4 6 diamino 2 phenylindole dapi - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    SouthernBiotech dapi fluoromount g

    Dapi Fluoromount G, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI+Fluoromount-G/pmc13657110-7-0-3
    Average 96 stars, based on 1 article reviews
    dapi fluoromount g - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Beyotime dapi
    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole <t>(DAPI)</t> <t>staining</t> at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).
    Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI/pmc13657358-230-4-5
    Average 99 stars, based on 1 article reviews
    dapi - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    96
    Vector Laboratories vectashield vibrance antifade mounting medium with dapi
    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
    Vectashield Vibrance Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/VECTASHIELD+Vibrance+Antifade+Mounting+Medium+with+DAPI/pmc13657221-53-0-8
    Average 96 stars, based on 1 article reviews
    vectashield vibrance antifade mounting medium with dapi - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    98
    Vector Laboratories dapi vector laboratories
    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
    Dapi Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm42861405-520-7-8
    Average 98 stars, based on 1 article reviews
    dapi vector laboratories - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    Image Search Results


    Journal: iScience

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

    doi: 10.1016/j.isci.2026.117149

    Figure Lengend Snippet:

    Article Snippet: 4′,6-diamino-2-phenylindole (DAPI) , Beyotime Biotechnology, China , Cat#C1002.

    Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software

    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: DAPI Fluoromount-G , SouthernBiotech, Birmingham, Alabama, USA , Cat#0100–20.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon

    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet: IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Article Snippet: Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark.

    Techniques: Migration, Control, Expressing, Analysis, EdU Assay, Fluorescence, Staining, Transwell Migration Assay, Transwell Assay

    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Morphology, Suspension, Microscopy, Scanning Electron Microscopy, Transmission Assay, Electron Microscopy, Brightfield, Analysis, Confocal, Staining

    Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Suspension, Confocal, Staining, Expressing, Incubation, Widefield, Fluorescence, Microscopy

    BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Expressing, Activity Assay, Suspension, Inhibition, Knock-Out, Confocal, Staining

    CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Activity Assay, Suspension, Confocal, Staining, Expressing, Concentration Assay, MANN-WHITNEY, Drug discovery

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Tissue, Recombinant, Membrane, Gentle, Cell Recovery, Staining, Plasmid Preparation, Saline, Viability Assay, Lysis, Mass Spectrometry, Software