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diamidin 2 phenylindol  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc diamidin 2 phenylindol

    Diamidin 2 Phenylindol, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI+solution/pmc13675480-527-5-7
    Average 99 stars, based on 121 article reviews
    diamidin 2 phenylindol - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa"

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    Journal: iScience

    doi: 10.1016/j.isci.2026.117730


    Figure Legend Snippet:

    Techniques Used: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    Related Articles

    Incubation:

    Article Title: Itgb3 Expression is Developmentally Regulated in Pyramidal Neurons of Mouse Cortex and Hippocampus
    Article Snippet: For double labeled experiments, we utilized mice ages P7-P14, and a second probe, such as Grm5 (ACD Bio, cat. #423631), Gria2 (ACD Bio, cat. #416091-C3), or Fmr1 (ACD Bio, cat. #496391-C2), was used with TSA Vivid 570 (ACD Bio, cat. #7535) after Itgb3 was detected utilizing TSA Vivid 650. .. After fluorescent detection of mRNAs, all tissue was incubated with DAPI solution (ACD Bio) for 30 seconds, excess DAPI was tapped off, the tissue was coated with ProLong Diamond (Invitrogen) and covered with a #1.5H thickness coverslip. ..

    Article Title: Amygdalostriatal transition zone neurons encode sustained cue responses to guide defensive behaviors.
    Article Snippet: .. Slides were then incubated in ACDbio DAPI for 10 minutes, washed, dried for 20 minutes, coverslipped with PVA-DABCO (Millipore-Sigma), and left to dry overnight before imaging. .. Confocal microscopy For the Drd1a/Drd2 labelling experiment, confocal fluorescence images were acquired on an Olympus FV1000 confocal laser scanning microscope using a 40x/1.30NA oil immersion objective, and serial Z-stack images were acquired using the FluoView software (Olympus, Center Valley, PA) at a thickness of 1.5 μm per Z-slice, with only the best-targeted Z-slice used for each target area.

    Imaging:

    Article Title: Amygdalostriatal transition zone neurons encode sustained cue responses to guide defensive behaviors.
    Article Snippet: .. Slides were then incubated in ACDbio DAPI for 10 minutes, washed, dried for 20 minutes, coverslipped with PVA-DABCO (Millipore-Sigma), and left to dry overnight before imaging. .. Confocal microscopy For the Drd1a/Drd2 labelling experiment, confocal fluorescence images were acquired on an Olympus FV1000 confocal laser scanning microscope using a 40x/1.30NA oil immersion objective, and serial Z-stack images were acquired using the FluoView software (Olympus, Center Valley, PA) at a thickness of 1.5 μm per Z-slice, with only the best-targeted Z-slice used for each target area.

    other:

    Article Title: A dual pronged approach to cancer immune exclusion: tumor-derived LAIR-1 simultaneously drives fibrosis and blocks immune recruitment in glioma
    Article Snippet: RNAscope in-situ hybridization was performed on 5-μm coronal cryosections mounted onto positively charged slides, following the manufacturer’s protocol (ACDBio).

    Article Title: Ependymomas are cancers of the pre-neural crest/roof plate lineage
    Article Snippet: DAB solution (DAKO Omnis) was applied for approximately 2-3 minutes before washing and hematoxylin staining for one minute.



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    Image Search Results


    ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Journal: Advanced Science

    Article Title: Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis

    doi: 10.1002/advs.78329

    Figure Lengend Snippet: ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Article Snippet: Cell nuclei were subsequently counterstained with DAPI (SBA‐0100‐20, 1:1000, SouthernBiotech).

    Techniques: Expressing, Western Blot, Analysis, Immunofluorescence, Marker, Multiplex Assay