dapi (SouthernBiotech)

SouthernBiotech is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

SouthernBiotech dapi

ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were <t>counterstained</t> with <t>DAPI</t> (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

Dapi, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi/DAPI+Fluoromount-G/pmc13677000-423-6-9
Average 96 stars, based on 4753 article reviews

dapi - by Bioz Stars, 2026-11

96/100 stars

Images

1) Product Images from "Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis"

Article Title: Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis

Journal: Advanced Science

doi: 10.1002/advs.78329

Figure Legend Snippet: ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

Techniques Used: Expressing, Western Blot, Analysis, Immunofluorescence, Marker, Multiplex Assay

other:

Article Title: CD47 fuels intestinal fibrosis by inducing fibroblast phagocytic evasion and represents a therapeutic target.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FACS-isolated mouse intestinal macrophages This paper Sorted from mouse colon single-cell suspensions for qPCR and SMART-seq/RNA-seq analyses Chemicals, peptides, and recombinant proteins Dextran sulfate sodium (DSS) MP Biomedicals Cat# 160110 2,4,6-trinitrobenzene sulfonic acid (TNBS) Meilunbio Cat# MB5523 Tamoxifen Sigma-Aldrich Cat# T5648 RNAlater Sigma-Aldrich Cat# R0901 Dulbecco’s Modified Eagle Medium (DMEM) Thermofisher Cat# 11960044 α-MEM KeyGEN BioTECH Cat# KGM11900-1 RPMI 1640 medium Thermofisher Cat# 22400089 Fetal bovine serum (FBS) Gibco Cat# 10091148 Penicillin/streptomycin KeyGEN BioTECH Cat# KGY0023 Murine M-CSF PeproTech Cat# 315-02-100 Recombinant TGF-β1 eBioscience Cat# 14-8342-80 Red blood cell lysis buffer Invitrogen Cat# 00-4300-54 Collagenase VIII Sigma-Aldrich Cat# C2139 DNase I Roche Cat# 5952077103 CFSE eBioscience Cat# 65-0850-84 Phosphate-buffered saline (PBS) Gibco Cat# 10010023 EDTA Gibco Cat# 15575020 Triton X-100 Beyotime Cat# P0096 DAPI (nuclear counterstain and flowcytometric viability dye) Sigma-Aldrich Cat# D9542 Fluoromount-G SouthernBiotech Cat# 0100-20 2× TransDirect Mouse Genotyping SuperMix TransGen Cat# AD501; used for mouse genotyping PCR 2× Phanta Flash Master Mix Vazyme Cat# P510; used for Col1a1-IRES-EGFP- IRES-MerCreMer mouse genotyping PCR MitoSOX Red Mitochondrial Superoxide Indicator Invitrogen/Thermo Fisher Scientific Cat# M36008 Critical commercial assays PanoPANEL kit Panovue Cat# 10234100050 Mouse IL-1β ELISA kit Sigma-Aldrich Cat# RAB0274 Mouse M-CSF/CSF1 ELISA kit Invitrogen/Thermo Fisher Scientific Cat# EMCSF1 Mouse TGF-β1 ELISA kit Invitrogen/Thermo Fisher Scientific Cat# BMS608-4 Deposited data Public human intestinal transcriptomic dataset GSE112366 NCBI Gene Expression Omnibus GEO: GSE112366 Public human intestinal transcriptomic dataset GSE16879 NCBI Gene Expression Omnibus GEO: GSE16879 Published human intestinal single-cell RNA-seq dataset Genome Sequence Archive GSA-Human: HRA006083; BioProject: PRJCA021346 Published mouse intestinal single-cell RNA-seq dataset Genome Sequence Archive GSA: CRA016292; BioProject: PRJCA021346 Published stricturing Crohn’s disease Visium spatial transcriptomic dataset Single Cell Portal Single Cell Portal: SCP2959 Published Crohn’s disease CosMx NanoString spatial dataset NCBI Gene Expression Omnibus GEO: GSE234713 (Continued on next page) e2 Cell Reports Medicine 7, 103103, October 20, 2026

Staining:

Article Title: Irisin promotes lipid clearance through the CD81-integrin-FAK axis in steatotic hepatocytes.
Article Snippet: Extended author information available on the last page of the article Abstract Irisin, an exercise-induced myokine, regulates metabolic homeostasis, but its receptor mechanisms in metabolic dysfunction-associated steatotic liver disease (MASLD) remain unclear.. This study investigates the role of the co-receptor CD81 in modulating irisin signaling and intrahepatic lipid homeostasis.. Clinical correlations were analyzed in 88 bariatric surgery patients and liver biopsy cohorts (n = 62).

Article Title: Temozolomide reshapes concentration, morphology, proteome, and biological activity of glioblastoma-derived extracellular vesicles
Article Snippet: 5 × 105 of GBM cells, BMDCs, or BV-2 cells were then exposed to PKH26labelled EVs (4 μg/mL), fixed at different time points (30 min, 1, 3, and 24 h) with 4% PFA, washed twice with PBS and blocked with 5% BSA in PBS, and then samples were stained for β-actin (1:200; AC004, ABclonal, Wuhan, China), washed twice with PBS and incubated with secondary Goat Anti-Mouse IgG H&L fluorescein isothiocyanate (FITC)-conjugated antibody (1:400; ab6785, Abcam, Cambridge, UK). .. Finally, nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (SouthernBiotech, Birmingham, AL). .. EV uptake was examined by confocal laser scanning microscopy (Leica Geosystems, Wetzlar, Germany) or Axio Imager M2 equipped with Apotome 2 (Zeiss GmbH, Jena, Germany).

Fluorescence:

Microscopy:

Glass:

Article Title: An aging-sensitive secreted phospholipase confers neuroprotection and cognitive resilience.
Article Snippet: Fluorescently labeled secondary antibodies ( Jackson ImmunoResearch) were used at a final concentration of 1:500 in PBS/glycerol. .. Sections were mounted on glass slides and coverslipped with mounting medium containing DAPI (Fluoromount with DAPI, Southern Biotech). .. For lipid droplets, stainings of 60-mm vibratome sections were obtained (Leica VT1200S) and stored in PBS at 4 °C.

Review