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dapi fluoromount g  (SouthernBiotech)


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    Structured Review

    SouthernBiotech dapi fluoromount g

    Dapi Fluoromount G, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 4703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI+Fluoromount-G/pmc13657110-7-0-3
    Average 96 stars, based on 4703 article reviews
    dapi fluoromount g - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models"

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    Journal: iScience

    doi: 10.1016/j.isci.2026.117673


    Figure Legend Snippet:

    Techniques Used: Recombinant, Mutagenesis, Software, Analysis, Silicon

    Related Articles

    Incubation:

    Article Title: Mitochondrial DNA released by Biliary Epithelial Cells enhances inflammation in Biliary Atresia through the cGAS-STING-NF-κB pathway.
    Article Snippet: .. After washing, the samples were 390 incubated with fluorophore-conjugated secondary antibodies for 1 h at room temperature, 391 followed by counterstaining and mounting with DAPI Fluoromount-G (0100-20, 392 SouthernBiotech). ..

    Article Title: MINOS1-mediated mitochondrial remodeling: A critical regulator of apoptosis in hypoxic intestinal epithelium.
    Article Snippet: Intestinal epithelial cells (IECs) are highly vulnerable to mitochondrial dysfunction and lethal apoptosis under severe hypoxic stress.. However, the upstream genetic determinants governing mucosal redox resilience remain poorly defined.. To investigate the mechanisms of intestinal epithelial cell apoptosis under cobalt chloride (CoCl2)-induced hypoxic conditions, we established a CoCl2-induced lethal hypoxia model in IPEC-J2 cells and performed a genome-wide CRISPR-Cas9 screen.

    Article Title: PAD2 Deficiency Reveals Sex-Divergent Colonic Homeostasis, β-Catenin Remodeling, and Antibiotic-Sensitive Phenotypes
    Article Snippet: Mouse-on-Mouse reagent (Vector Laboratories) was used where appropriate. .. Sections were incubated with primary antibodies [1:100] overnight at 4 ◦C, followed by Alexa https://doi.org/10.3390/biom16101415 Fluor-conjugated secondary antibodies [1:1000] (Table A1) and mounted with Fluoromount+DAPI (SouthernBiotech, Birmingham, AL, USA). .. Images were acquired on a Leica Stellaris 5 confocal microscope (Wetzlar, Germany) using a 20× or 63× objective, with acquisition settings held constant within each experiment.

    Control:

    Article Title: CDK7, CDK9, or CDK11 inhibition reduces neutrophil-driven inflammation and tissue damage in experimental autoimmune models
    Article Snippet: Platelet counts were determined using an ADVIA 2120i hematocytometer (Siemens Healthineers, Erlangen, Germany). .. Paraffin-embedded ear sections of MC180295 -treated and control antibody transfer-induced EBA mice or paw sections of KBxN STA mice were subjected to immunofluorescence staining for Ly6G or F4/80 as described previously [ ] and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama, USA). .. Images were taken at a Keyence BZ-X810 Fluorescence microscope (Osaka, Japan) and quantified using ImageJ software version 1.53p (National Institutes of Health, Bethesda, Maryland, USA).

    Immunofluorescence:

    Article Title: CDK7, CDK9, or CDK11 inhibition reduces neutrophil-driven inflammation and tissue damage in experimental autoimmune models
    Article Snippet: Platelet counts were determined using an ADVIA 2120i hematocytometer (Siemens Healthineers, Erlangen, Germany). .. Paraffin-embedded ear sections of MC180295 -treated and control antibody transfer-induced EBA mice or paw sections of KBxN STA mice were subjected to immunofluorescence staining for Ly6G or F4/80 as described previously [ ] and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama, USA). .. Images were taken at a Keyence BZ-X810 Fluorescence microscope (Osaka, Japan) and quantified using ImageJ software version 1.53p (National Institutes of Health, Bethesda, Maryland, USA).

    Staining:

    Article Title: CDK7, CDK9, or CDK11 inhibition reduces neutrophil-driven inflammation and tissue damage in experimental autoimmune models
    Article Snippet: Platelet counts were determined using an ADVIA 2120i hematocytometer (Siemens Healthineers, Erlangen, Germany). .. Paraffin-embedded ear sections of MC180295 -treated and control antibody transfer-induced EBA mice or paw sections of KBxN STA mice were subjected to immunofluorescence staining for Ly6G or F4/80 as described previously [ ] and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama, USA). .. Images were taken at a Keyence BZ-X810 Fluorescence microscope (Osaka, Japan) and quantified using ImageJ software version 1.53p (National Institutes of Health, Bethesda, Maryland, USA).

    Article Title: Limiting the intracellular calcium accumulation alleviates fibroblast senescence and pulmonary fibrosis.
    Article Snippet: Activation of specific calcium regulators, along with their downstream intracellular calcium signaling pathways has been established as a hallmark feature of cellular senescence.. Nevertheless, alternations in intracellular free calcium concentration and the functional consequences thereof during cellular senescence and age-related diseases remain incompletely understood, particularly in fibroblasts.. In this study, we measured intracellular calcium levels during senescence and observed a significant increase.

    Article Title: A basolateral amygdala to dorsolateral striatum projection modulates stimulus-evoked motor behavior
    Article Snippet: .. Stained sections were rinsed with PBS (1x 10 min), mounted onto microscope slides (Fisherbrand Superfrost Plus) and cover-slipped with Fluoromount-G with 4′,6-diamidino-2-phenylindole (DAPI) (Southern Biotech). ..

    Fluorescence:

    Article Title: MINOS1-mediated mitochondrial remodeling: A critical regulator of apoptosis in hypoxic intestinal epithelium.
    Article Snippet: Intestinal epithelial cells (IECs) are highly vulnerable to mitochondrial dysfunction and lethal apoptosis under severe hypoxic stress.. However, the upstream genetic determinants governing mucosal redox resilience remain poorly defined.. To investigate the mechanisms of intestinal epithelial cell apoptosis under cobalt chloride (CoCl2)-induced hypoxic conditions, we established a CoCl2-induced lethal hypoxia model in IPEC-J2 cells and performed a genome-wide CRISPR-Cas9 screen.

    Microscopy:

    Article Title: A basolateral amygdala to dorsolateral striatum projection modulates stimulus-evoked motor behavior
    Article Snippet: .. Stained sections were rinsed with PBS (1x 10 min), mounted onto microscope slides (Fisherbrand Superfrost Plus) and cover-slipped with Fluoromount-G with 4′,6-diamidino-2-phenylindole (DAPI) (Southern Biotech). ..



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    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole <t>(DAPI)</t> <t>staining</t> at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).
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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Image Search Results


    Journal: iScience

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

    doi: 10.1016/j.isci.2026.117149

    Figure Lengend Snippet:

    Article Snippet: 4′,6-diamino-2-phenylindole (DAPI) , Beyotime Biotechnology, China , Cat#C1002.

    Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software

    Journal: iScience

    Article Title: Midline thalamic neurons show early hypersynchrony during absence seizures in mouse models

    doi: 10.1016/j.isci.2026.117673

    Figure Lengend Snippet:

    Article Snippet: DAPI Fluoromount-G , SouthernBiotech, Birmingham, Alabama, USA , Cat#0100–20.

    Techniques: Recombinant, Mutagenesis, Software, Analysis, Silicon

    IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Journal: iScience

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment

    doi: 10.1016/j.isci.2026.117739

    Figure Lengend Snippet: IL-4 and Arg remodel macrophages and promote their migration (A) Schematic illustration of the regulatory role of IL-4 on macrophage phenotype. (B) Volcano plot displaying differentially expressed genes (DEGs) between the control and IL-4-treated macrophages. (C) Heatmap demonstrating the expression of key macrophage polarization markers. (D) Multi-pathway Gene Set Enrichment Analysis (GSEA) demonstrating the integrated enrichment of indicated pathways regulating macrophage functions. (E) Schematic illustration of macrophage priming for efferocytosis mediated by IL-4 and Arg. (F) Relative cell viability of macrophages treated with different concentrations of Arg at 24, 48, and 72 h. (G and H) Macrophage proliferation evaluated by 5-ethynyl-2′-deoxyuridine (EdU) assay, showing representative fluorescence images of EdU and 4',6-diamidino-2-phenylindole (DAPI) staining at 24, 48, and 72 h (G) and quantification of EdU-positive cells treated with 2 mM Arg (H). (I) Schematic illustration of the Transwell migration assay. (J and K) Macrophage recruitment evaluated by Transwell assay, showing representative crystal violet staining images of migrated macrophages after 48 h (J) and quantification of the number of migrated macrophages (K). Error bars represent SD. Data were analyzed by two-way ANOVA followed by Sidak’s multiple comparisons test (F and H) or one-way ANOVA followed by Tukey’s post hoc test (K). Sample sizes: n = 3 biological replicates (B–D and F); n = 5 biological replicates, each performed with 3 technical replicates (G, H, J, and K). Scale bars, 25 μm (G), 500 μm (J, top), and 100 μm (J, bottom).

    Article Snippet: Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark.

    Techniques: Migration, Control, Expressing, Analysis, EdU Assay, Fluorescence, Staining, Transwell Migration Assay, Transwell Assay

    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Morphology, Suspension, Microscopy, Scanning Electron Microscopy, Transmission Assay, Electron Microscopy, Brightfield, Analysis, Confocal, Staining

    Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Suspension, Confocal, Staining, Expressing, Incubation, Widefield, Fluorescence, Microscopy

    BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Expressing, Activity Assay, Suspension, Inhibition, Knock-Out, Confocal, Staining

    CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Activity Assay, Suspension, Confocal, Staining, Expressing, Concentration Assay, MANN-WHITNEY, Drug discovery

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Tissue, Recombinant, Membrane, Gentle, Cell Recovery, Staining, Plasmid Preparation, Saline, Viability Assay, Lysis, Mass Spectrometry, Software