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antifading mounting medium containing 4',6-diamidino-2-phenylindole (dapi) solution  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science antifading mounting medium containing 4',6-diamidino-2-phenylindole (dapi) solution
    Antifading Mounting Medium Containing 4',6 Diamidino 2 Phenylindole (Dapi) Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi-containing+antifade+mounting+solution/dapi+s2110/pm37890686-82-22-28
    Average 90 stars, based on 1 article reviews
    antifading mounting medium containing 4',6-diamidino-2-phenylindole (dapi) solution - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
    Article Snippet: .. The cells were incubated overnight with USP10 and Mfn2 antibodies, followed by incubation with fluorescent secondary antibodies Alexa Fluor 488 (Beyotime, P0188) and Alexa Fluor 555 (Beyotime, A0453) at 37 °C for 1 h. Cell nuclei were stained with DAPI (Solarbio, S2110), and F-actin was stained with Actin-Tracker Red-Rhodamine (Beyotime, C2207S). .. Microscopic images were magnified × 400 using an intelligent biological microscope (OLYMPUS BX53).

    Article Title: STAT1-IFITM3 promotes autophagy in epithelial cells to control Cryptosporidium parvum infection
    Article Snippet: For cytoskeleton staining, 200 μl of iFluor 546-Ghost cyclic peptide F-actin (Creative Biolabs) working solution was added to the cells and stained at RT for 15 min, followed by PBS washing. .. Subsequently, a drop of antifading mounting medium with the nucleic acid stain DAPI (Solarbio) was added to seal the slide. .. Finally, cell immunofluorescence was assessed using the Invitrogen EVOS FL Auto Cell Imaging System or a Nikon eclipse Ti Confocal Microscope.

    Fluorescence:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: CTSK as a Collagen Degradation Marker Induces Gingival Recession During High-Force Orthodontic Tooth Movement
    Article Snippet: .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B). .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B).

    Microscopy:

    Article Title: Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.
    Article Snippet: Next, cells were incubated with the rabbit polyclonal to lc3B (1:200, #ab48394, abcam) at 4 °c overnight. subsequently, cells were washed with PBs for three times and treated with Goat anti-Rabbit igG h&l (alexa Fluor® 488) (1:1000, #ab150077, abcam) for 1 h at room temperature. .. Finally, cells were stained with Mounting Medium, antifading with DaPi (#s2110, solarbio) and photographed under a fluorescence microscopy (#iX71, Olympus, tokyo, Japan). ..

    Article Title: CTSK as a Collagen Degradation Marker Induces Gingival Recession During High-Force Orthodontic Tooth Movement
    Article Snippet: .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B). .. The sections were mounted with DAPI-containing antifade mounting medium (Solarbio, Beijing), pressed overnight, and observed and imaged under an upright fluorescence microscope (Leica, DM4B).

    other:

    Article Title: Protective effect and mechanism of Zhuang medicine Tinosporae Radix on LPS-induced neuroinflammation.
    Article Snippet: Ethnopharmacological relevance: In Zhuang medicine, Alzheimer’s disease (AD) is termed “Qiaowuwa’, attributed to the accumulation of heat-toxin in the Huolu (Fire Pathway) and malnutrition of Qiaowu.. The dried tuberous roots of Tinospora sagittata (Oliv.). Gagnep., known as “Tinosporae Radix’, are documented in Zhuang medicinal texts for their bitter flavor and cold nature, which clear heat-toxin, regulate Huolu, and treat inflammatory disorders.

    Article Title: The substructure of the endothelial glycocalyx in rat aorta and its interaction with the low-density lipoproteins (LDL).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Vascularized Bioengineered Kidney Using Decellularized Scaffold Recellularized with human Placenta-Derived Angiogenic stem Cells and Kidney Organoids
    Article Snippet: Permeabilize with 0.25% Triton X-100 (Beyotime, P0096) for 5-15 minutes, followed by washing with PBS containing 0.1% Tween-20 (Solarbio, T8220, PBST).

    Article Title: Tanshinone IIA induces oxidative stress in trophoblast cells and enhances copper dependent death.
    Article Snippet: After washing, antifading mounting medium containing DAPI (Solarbio, S2110) was added dropwise to the slides.

    Incubation:

    Article Title: USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
    Article Snippet: .. The cells were incubated overnight with USP10 and Mfn2 antibodies, followed by incubation with fluorescent secondary antibodies Alexa Fluor 488 (Beyotime, P0188) and Alexa Fluor 555 (Beyotime, A0453) at 37 °C for 1 h. Cell nuclei were stained with DAPI (Solarbio, S2110), and F-actin was stained with Actin-Tracker Red-Rhodamine (Beyotime, C2207S). .. Microscopic images were magnified × 400 using an intelligent biological microscope (OLYMPUS BX53).



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    Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) <t>Representative</t> <t>fluorescence</t> microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). <t>DAPI-stained</t> nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.
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    Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) <t>Representative</t> <t>fluorescence</t> microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). <t>DAPI-stained</t> nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.
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    Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) <t>Representative</t> <t>fluorescence</t> microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). <t>DAPI-stained</t> nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.
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    Image Search Results


    Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) Representative fluorescence microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). DAPI-stained nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.

    Journal: Pharmaceutics

    Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

    doi: 10.3390/pharmaceutics15061651

    Figure Lengend Snippet: Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) Representative fluorescence microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). DAPI-stained nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.

    Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

    Techniques: Incubation, Fluorescence, Microscopy, Transmission Assay, Staining

    Digitoxin-loaded hTf-QDs reduced A549 cell number. Left panel: Representative fluorescence microscopy images (20×) of 16HB14o- and A549 cells incubated for 48 h without (Control, ( A , B ) panels) or with hTf-QDs (( C , D ) panels). hTf-QDs are visualized in red and DAPI-stained nuclei are shown in blue. ( E ) Effect of hTf-QDs loaded with digitoxin (hTf-QDs-Dig) on 16HB14o- (green) and A549 (red) cells treated for 48 h. As a control, cells were incubated without hTf-QDs (Control) or in the presence of hTf-QDs without digitoxin (hTf-QDs). * indicates significative difference between treatments.

    Journal: Pharmaceutics

    Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

    doi: 10.3390/pharmaceutics15061651

    Figure Lengend Snippet: Digitoxin-loaded hTf-QDs reduced A549 cell number. Left panel: Representative fluorescence microscopy images (20×) of 16HB14o- and A549 cells incubated for 48 h without (Control, ( A , B ) panels) or with hTf-QDs (( C , D ) panels). hTf-QDs are visualized in red and DAPI-stained nuclei are shown in blue. ( E ) Effect of hTf-QDs loaded with digitoxin (hTf-QDs-Dig) on 16HB14o- (green) and A549 (red) cells treated for 48 h. As a control, cells were incubated without hTf-QDs (Control) or in the presence of hTf-QDs without digitoxin (hTf-QDs). * indicates significative difference between treatments.

    Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

    Techniques: Fluorescence, Microscopy, Incubation, Staining