dapi staining solution containing antifade mounting medium (Servicebio Inc)
90
Structured Review
Servicebio Inc
dapi staining solution containing antifade mounting medium
Dapi Staining Solution Containing Antifade Mounting Medium, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi-containing+antifade+mounting+solution/dapi/pmc11191282-87-10-15
Average 90 stars, based on 1 article reviews
Dapi Staining Solution Containing Antifade Mounting Medium, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi-containing+antifade+mounting+solution/dapi/pmc11191282-87-10-15
Average 90 stars, based on 1 article reviews
dapi staining solution containing antifade mounting medium - by Bioz Stars,
2026-11
90/100 stars
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Staining:Article Title: Harnessing the gut–immune–joint axis: Oral microalgae-based thermoresponsive microspheres enhance intra-articular therapy for rheumatoid arthritis Article Snippet: After 2 h, basolateral samples were collected and fluorescence intensity was recorded (Ex/Em = 492/520 nm) using a microplate reader (SpectraMax iD5, Molecular Devices, USA). .. For tight junction (TJ) protein detection, Caco-2 monolayers were fixed and stained with antibodies against Claudin-1 (Abnova, China), Occludin (Abcam, UK), and ZO-1 (Abcam, UK), followed by Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity Article Snippet: After removal of the primary antibody, cells were incubated with a fluorescently labeled secondary antibody (AS039, ABclonal) at room temperature for 60 minutes protected from light. .. Finally, nuclei were stained with Article Title: Sensory nerve-derived semaphorin 3A promotes bone healing through macrophage immunomodulation. Article Snippet: Sensory innervation has emerged as a crucial regulator of skeletal homeostasis and regeneration.. Growing evidence indicates that disruption of sensory nerves is associated with excessive inflammation and impaired tissue healing.. However, the underlying mechanisms by which neuronal signaling regulates the immune microenvironment to orchestrate bone repair remain unclear. Incubation:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with Confocal:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with Microscopy:Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100). .. After three PBS washes, fluorescent secondary antibodies (Servicebio, Alexa Fluor 488, GB25303; Alexa Fluor 594, GB28303) were incubated for 1 h in the dark, nuclei were counterstained with |
