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prolong gold antifade mounting medium  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc prolong gold antifade mounting medium
    Prolong Gold Antifade Mounting Medium, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 2159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi-containing+antifade+mounting+solution/DAPI/pmc07432669-367-54-59
    Average 98 stars, based on 2159 article reviews
    prolong gold antifade mounting medium - by Bioz Stars, 2026-11
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Metabolic‐Redox Remodeling of Inflammatory Synovium by Mannose‐Modified Selenium Nanoparticles for Osteoarthritis Therapy
    Article Snippet: .. Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X‐100, and stained with Actin‐Tracker Green‐488 (1:200, Beyotime) and DAPI (1:1000, CST). .. Cellular internalization was observed via a Leica STELLARIS 5 confocal microscope and quantitatively assessed by flow cytometry (BD LSRFortessa) with DiD fluorescence measured in the APC channel.

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: .. Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. ..

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: In brief, Adenoassociated virus (AAV) vector-mediated overexpression of mouse RUNX1, control (ZsGreen), Adenoassociated virus (AAV)-Cre-ZsGreen and Control (ZsGreen) were purchased from Hanbio biotechnology (Shanghai, China). .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature. .. Nile red staining Cells were washed once with PBS, fixed in 4% paraformaldehyde for 15 min and stained with 0.5 μM Nile red solution (Sigma, N3013) and 1ng/mL DAPI (CST, 4083) for 10 minutes at ambient temperature.

    Confocal:

    Article Title: Ondansetron reduces seizures and improves cognitive impairment in a rat model of temporal lobe epilepsy
    Article Snippet: After PBS washes, samples were incubated with Alexa Fluor-conjugated secondary antibodies (goat anti-mouse 555 and goat anti-rabbit 647, 1:1000, CST) for 1 hr at RT in the dark. .. Nuclei were counterstained with DAPI (CST) for 10 min. Confocal images were acquired at 60 × magnification. .. The rats were uninterruptedly recorded using a 4-camera video system (Hikvision, China) in transparent cages.

    Incubation:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).

    Blocking Assay:

    Article Title: Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanisms.
    Article Snippet: Images were acquired using a digital slide scanner (Axio Scan Z1, Carl Zeiss). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083). .. Cells were then incubated with primary antibodies against PPARγ diluted in blocking buffer at 4 °C overnight, followed by incubation with appropriate fluorophoreconjugated secondary antibodies at room temperature for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, 4083).



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    Image Search Results


    Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) Representative fluorescence microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). DAPI-stained nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.

    Journal: Pharmaceutics

    Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

    doi: 10.3390/pharmaceutics15061651

    Figure Lengend Snippet: Temperature-dependent incorporation of hTf-QDs is higher than HSA-QDs in A549 cells. Cells were co-incubated with hTf-QDs (red) and HSA-QDs (green) for 3 h. ( A ) Representative plot showing the frequency of the number of NPs per A549 cell (quantification of three independent experiments are shown in panels ( E , F )). ( B – D ) Representative fluorescence microscopy images using UV and transmission light simultaneously of A549 cells at 4 °C ( B ), 37 °C ( C ), and 16HB14o- cells at 37 °C ( D ). The co-localization of two HSA-QDs and hTf-QDs is visualized in yellow (arrow). DAPI-stained nuclei are shown in blue. ( E ) Box plot of HSA-QDs and hTf-QDs per A549 cell incubated at 4 °C or 37 °C during 3 h. The box comprises the 2nd and 3rd quartile of the data and the horizontal thick line defines the median. Outliers (>1.5× interquartile range are marked by diamonds). ( F ) Box plot of the average number of HSA-QDs and hTf-QD per 16HB14o- cell incubated at 37 °C for 3 h.

    Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

    Techniques: Incubation, Fluorescence, Microscopy, Transmission Assay, Staining

    Digitoxin-loaded hTf-QDs reduced A549 cell number. Left panel: Representative fluorescence microscopy images (20×) of 16HB14o- and A549 cells incubated for 48 h without (Control, ( A , B ) panels) or with hTf-QDs (( C , D ) panels). hTf-QDs are visualized in red and DAPI-stained nuclei are shown in blue. ( E ) Effect of hTf-QDs loaded with digitoxin (hTf-QDs-Dig) on 16HB14o- (green) and A549 (red) cells treated for 48 h. As a control, cells were incubated without hTf-QDs (Control) or in the presence of hTf-QDs without digitoxin (hTf-QDs). * indicates significative difference between treatments.

    Journal: Pharmaceutics

    Article Title: Two-Step Preparation of Protein-Decorated Biohybrid Quantum Dot Nanoparticles for Cellular Uptake

    doi: 10.3390/pharmaceutics15061651

    Figure Lengend Snippet: Digitoxin-loaded hTf-QDs reduced A549 cell number. Left panel: Representative fluorescence microscopy images (20×) of 16HB14o- and A549 cells incubated for 48 h without (Control, ( A , B ) panels) or with hTf-QDs (( C , D ) panels). hTf-QDs are visualized in red and DAPI-stained nuclei are shown in blue. ( E ) Effect of hTf-QDs loaded with digitoxin (hTf-QDs-Dig) on 16HB14o- (green) and A549 (red) cells treated for 48 h. As a control, cells were incubated without hTf-QDs (Control) or in the presence of hTf-QDs without digitoxin (hTf-QDs). * indicates significative difference between treatments.

    Article Snippet: For fluorescence microscopic visualization, the slides were covered with DAPI-containing antifade mounting solution (ProLong ® Gold antifade reagent, Invitrogen).

    Techniques: Fluorescence, Microscopy, Incubation, Staining