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6 diamidino 2 phenylindole dapi staining solution  (Boster Bio)


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    Structured Review

    Boster Bio 6 diamidino 2 phenylindole dapi staining solution
    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
    6 Diamidino 2 Phenylindole Dapi Staining Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+4/DAPI+(4'%2C6-Diamidino-2-Phenylindole%2C+Dihydrochloride)+for+nucleic+acid+staining/pmc12960002-54-68-73
    Average 96 stars, based on 760 article reviews
    6 diamidino 2 phenylindole dapi staining solution - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models"

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models

    Journal: Reviews in Cardiovascular Medicine

    doi: 10.31083/RCM42804

    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.
    Figure Legend Snippet: Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

    Techniques Used: Expressing, Inhibition

    Related Articles

    Staining:

    Article Title: Kaempferol attenuates LPS-induced inflammatory responses in H9c2 cells through involvement of the IL-6/JAK2/STAT3 pathway
    Article Snippet: Lipopolysaccharide (LPS) (Cat# S11060) and Dexamethasone (Dex) (Cat# S17003) were obtained from the Nanjing Jiancheng Bioengineering Institute. .. CY3 Fluorescent Antibody (Cat# BA1032), β-actin (Cat# BM3873), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Assay Kit (Cat# AR0146), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Measurement Kit (Cat# AR0146) were purchased from BOSTER. .. Phospho-STAT3 antibody (Cat# 9145) was purchased from Cell Signaling Technology (Shanghai) Biological Reagents Co., Ltd. Phospho-JAK2 antibody (Cat# ab32101) was obtained from Abcam.

    Article Title: Rutin Maintains the Thermogenic Phenotype of Beige Adipocytes and Concomitantly Suppresses Mitophagy Against Obesity in HFD Mice.
    Article Snippet: .. Following this, a speciesspecific fluorescent secondary antibody (1:50 dilution) was added for 1 h. Finally, DAPI nuclear stain (BOSTER, Pleasanton, CA, USA; AR1176) was added for 30 min, and images were acquired using a microscope. ..

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models
    Article Snippet: Furthermore, an improved hematoxylin–eosin (HE) staining kit (G1121, Solarbio Technology Co., Ltd., Shanghai, China) and the modified Movat–Russell pentachrome staining kit (G3700, Solarbio Technology Co., Ltd., Shanghai, China) were purchased from Solarbio, China. .. In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China. .. The human collagen 1 ELISA kit (CSB-EL005716HU, CUSABIO BIOTECH CO., Ltd., Wuhan, China) was obtained from CUSABIO, China.

    Article Title: GOLGB1 deficiency accelerates intervertebral disc degeneration by activating the MAPK pathway.
    Article Snippet: .. Next, the nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI, AR1176, Boster) for 30 minutes. ..

    Protein Concentration:

    Article Title: Kaempferol attenuates LPS-induced inflammatory responses in H9c2 cells through involvement of the IL-6/JAK2/STAT3 pathway
    Article Snippet: Lipopolysaccharide (LPS) (Cat# S11060) and Dexamethasone (Dex) (Cat# S17003) were obtained from the Nanjing Jiancheng Bioengineering Institute. .. CY3 Fluorescent Antibody (Cat# BA1032), β-actin (Cat# BM3873), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Assay Kit (Cat# AR0146), DAPI Staining Solution (Cat# AR1176), BCA Protein Concentration Measurement Kit (Cat# AR0146) were purchased from BOSTER. .. Phospho-STAT3 antibody (Cat# 9145) was purchased from Cell Signaling Technology (Shanghai) Biological Reagents Co., Ltd. Phospho-JAK2 antibody (Cat# ab32101) was obtained from Abcam.

    Labeling:

    Article Title: proBDNF-SorCS2 Axis Suppresses Osteogenesis and Augments Inflammation of Human Periodontal Ligament Stem Cells in Inflammatory Conditions
    Article Snippet: For IF, the slices were subjected to incubation with secondary antibodies, including anti-rabbit FITC (Boster, #BA1105) or antimouse CY3 antibodies (Boster, #BA1031). .. The nuclei were labeled with DAPI (Boster, #AR1176). .. IHC images were obtained using the Nikon Microscope Image System (Nikon), and immunofluorescence-positive stained cells were observed using a confocal fluorescence microscope (Olympus).

    Microscopy:

    Article Title: Rutin Maintains the Thermogenic Phenotype of Beige Adipocytes and Concomitantly Suppresses Mitophagy Against Obesity in HFD Mice.
    Article Snippet: .. Following this, a speciesspecific fluorescent secondary antibody (1:50 dilution) was added for 1 h. Finally, DAPI nuclear stain (BOSTER, Pleasanton, CA, USA; AR1176) was added for 30 min, and images were acquired using a microscope. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models
    Article Snippet: Furthermore, an improved hematoxylin–eosin (HE) staining kit (G1121, Solarbio Technology Co., Ltd., Shanghai, China) and the modified Movat–Russell pentachrome staining kit (G3700, Solarbio Technology Co., Ltd., Shanghai, China) were purchased from Solarbio, China. .. In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China. .. The human collagen 1 ELISA kit (CSB-EL005716HU, CUSABIO BIOTECH CO., Ltd., Wuhan, China) was obtained from CUSABIO, China.

    Fluorescence:

    Article Title: Vitamin D3 mediates amelioration of ulcerative colitis via the TRPV1-MAPK signaling pathway
    Article Snippet: .. DAPI (Cat# AR1176, Boster Biological Technology) could staine both apoptotic and non-apoptotic nuclei blue, while the apoptotic cell nucleus is localized by green fluorescence with the incorporation of CF488-dUTP (Cat# G1504, Servicebio). ..



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    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
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    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
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    Image Search Results


    Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

    Journal: Reviews in Cardiovascular Medicine

    Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models

    doi: 10.31083/RCM42804

    Figure Lengend Snippet: Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

    Article Snippet: In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China.

    Techniques: Expressing, Inhibition