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Servicebio Inc dapi staining solution 4
Dapi Staining Solution 4, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pm42041094-297-31-35
Average 86 stars, based on 1 article reviews
dapi staining solution 4 - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Staining:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Evolutionary conservation and divergence of phagocytic and coagulation programs across bilaterian circulating immune cells.
Article Snippet: .. After being washed by the following SSC: 2 × SSC, 37°C 3 for 10 min, 1 × SSC, 37°C for 2 × 5 min, 0.5 × SSC 37°C for 10 min, DAPI staining solution 4 (Servicebio, China) was added to the cell slices in the dark condition for 8 min. ..

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Article Title: Bangia fusco-purpurea polysaccharides attenuate DSS-induced colitis in mice through gut microbiota and its metabolism modulation
Article Snippet: The next day, CY3-labeled goat anti-rabbit IgG (1:300) secondary antibody (Servicebio, Wuhan, China) was added and incubated in the dark at room temperature for 50 min. .. Subsequently, the sections were incubated with DAPI staining solution (Servicebio, Wuhan, China) in the dark at room temperature for 10 min, followed by the addition of autofluorescence quencher (G1221) (Servicebio, Wuhan, China) for 5 min and rinsing. .. Finally, the sections were covered with antifluorescence quencher mounting medium and images were obtained using an inverted fluorescence microscope (Nikon Eclipse C1, Tokyo, Japan).

Incubation:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Article Title: Bangia fusco-purpurea polysaccharides attenuate DSS-induced colitis in mice through gut microbiota and its metabolism modulation
Article Snippet: The next day, CY3-labeled goat anti-rabbit IgG (1:300) secondary antibody (Servicebio, Wuhan, China) was added and incubated in the dark at room temperature for 50 min. .. Subsequently, the sections were incubated with DAPI staining solution (Servicebio, Wuhan, China) in the dark at room temperature for 10 min, followed by the addition of autofluorescence quencher (G1221) (Servicebio, Wuhan, China) for 5 min and rinsing. .. Finally, the sections were covered with antifluorescence quencher mounting medium and images were obtained using an inverted fluorescence microscope (Nikon Eclipse C1, Tokyo, Japan).

Fluorescence:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Microscopy:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

other:

Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway
Article Snippet: The cells were then incubated with an anti-phospho-PERK primary rabbit antibody (1:100, Cell Signaling Technology, Cat# 3179S, RRID: AB_2095853) overnight at 4°C.

Inverted Microscopy:

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Sterility:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Cell Culture:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Membrane:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..



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Average 86 stars, based on 1 article reviews
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Image Search Results


LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Journal: International Journal of Nanomedicine

Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

doi: 10.2147/IJN.S645818

Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing