dapi staining solution (Merck KGaA)

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Merck KGaA dapi staining solution
Dapi Staining Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pmc08553967-47-14-18
Average 90 stars, based on 1 article reviews

dapi staining solution - by Bioz Stars, 2026-11

90/100 stars

Images

Staining:

Article Title: Monocytes in leukapheresis products affect the outcome of CD19–targeted CAR T-cell therapy in patients with lymphoma
Article Snippet: Data were acquired on BD FACSCanto II (BD Biosciences), MACSQuant Analyzer MQ10 or MQ16 (Miltenyi Biotec) and analyzed using FlowJo-v10 and MACSQuantify softwares. .. To visualize lymphocyte-monocyte complexes with the DEPArray (Menarini-Silicon Biosystems), thawed leukapheresis samples were stained with CD3-PE (REA613) and CD14-APC (REA599) antibodies (Miltenyi Biotec), fixed using flow cytometry fixation buffer (R&D Systems) and counterstained with 4',6-diamidino-2-phenylindole (DAPI) solution (Merck Millipore). ..

Article Title: CircFOXO3 protects against osteoarthritis by targeting its parental gene FOXO3 and activating PI3K/AKT-mediated autophagy.
Article Snippet: .. Subsequently, the slides were washed using PBS and incubated with DAPI solution (Merck KGaA, Darmstadt, Germany) for 5 min at RT in the dark to stain cell nuclei. ..

Article Title: Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species
Article Snippet: .. Small aliquots of Nicotiana spp. pollen of different stages (0.5–1 μL) were resuspended in DAPI staining solution (4′-6-Diamidino-2-phenylindole, Merck KGaA, Darmstadt, Germany) for cell nuclei visualization [16 μL of DAPI stock solution in 10 mL buffer, modified according to ] and Lugol’s staining solution for starch grains visualization (3.5% solution: 5 g KI, 2.5 g I, 200 ml 80% ethanol). .. Bright field and fluorescence (UV light) microscopy were used for checking the particular stage of tobacco pollen and starch content on an inverted fluorescent microscopes Nikon Eclipse TE2000E and Zeiss Axiovert 200M.

Flow Cytometry:

Incubation:

Article Title: Nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance of melanoma.
Article Snippet: As described previously,23 cells were cultivated on 8-well chamber slides (Corning Incorporated, Corning, USA) and incubated at 37 C. After 15 min of fixation with 4% paraformaldehyde, the cells were washed three times with PBS and blocked for 1 h with 1% BSA and 0.1% Triton-X-100 in PBS. .. Subsequently, cells were incubated with the anti-AREG antibody (Santa Cruz Biotechnology, Heidelberg, Germany; sc-74 501; 1 in 100 dilution), anti-V5 antibody (Cell Signaling Technology, Frankfurt am Main, Germany; #13202, 1 in 300 dilution) for 1 h at room temperature, and after three washing steps, the secondary antibody Alexa Fluor 488 (Thermo Fisher Scientific, Waltham, Massachusetts, USA; 1 in 200 dilution) was applied for 1 h. Finally, cells were incubated in DAPI solution (Merck KGaA, Darmstadt, Germany) in 1% BSA/PBS for 30 min followed by three times washing with PBS and Aqua-Poly/Mount (US Headquarters Polysciences, Warrington, PA) was used for mounting. .. Immunofluorescence staining was analyzed with an IX83 microscope (Olympus, Hamburg, Germany).

Article Title: Knockdown of Lamin B1 and the Corresponding Lamin B Receptor Leads to Changes in Heterochromatin State and Senescence Induction in Malignant Melanoma
Article Snippet: On the following day, the slides were incubated for 1 h with the secondary Cy3 antibody (1:500, Biozol, Eching, Germany). .. Subsequently, the slides were incubated in a DAPI solution (Merck KGaA, Darmstadt, Germany) in 1% BSA/PBS for 30 min. After every incubation step, the slides were washed several times with PBS. .. Aqua-Poly/Mount (US Headquarters Polysciences, Warrington, PA, USA) was used as the mounting medium.

Modification:

Starch:

other:

Article Title: Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells.
Article Snippet: D9542; Sigma-Aldrich; Merck KGaA) staining solution was added to the coverslips and incubated for 5 min at room temperature.

Article Title: Loss of miR-101-3p in melanoma stabilizes genomic integrity, leading to cell death prevention
Article Snippet: Thereafter, the cover slips were incubated in 4′,6-diamidino-2-phenylindole (DAPI) solution (1:10,000) (Merck KGaA, Darmstadt, Germany) in 1% BSA/PBS for 30 min. As mounting medium, Aqua-Poly/Mount (US Headquarters Polysciences, Warrington, PA, USA) was used.

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