dapi solution (4,6-diamidino-2-phenylindole dihydrochloride) (SERVA Electrophoresis)

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SERVA Electrophoresis dapi solution (4,6-diamidino-2-phenylindole dihydrochloride)

PARP protein levels and apoptosis rate of either non-irradiated or irradiated cells after siRNA transfection . A . Western blot analysis of PARP with rabbit anti-human cleaved PARP (Asp214) antibody in MDA-MB-321 cells 24 h and 72 h after transfection. The cells were untreated (UT), transfected with 100 nM of either nonsense siRNA (non) or target-specific siRNAs to knockdown OPN (Mix and OpnS). The Western blot shows one representative result out of three independent experiments. Actin served as an internal loading control. B . The morphology of <t>DAPI</t> stained cell nuclei was analyzed to quantify the apoptosis rate of MDA-MB-231 cells 72 h after transfection. The diagram shows the apoptosis rate of the cells as a function of treatment and irradiation. A <t>fluorescence</t> <t>microscope</t> was used and 500 cells in several fields of view were counted for each experiment. Data represent the average values (± SD) of three independent experiments (* p < 0.05, ** p < 0.001).

Dapi Solution (4,6 Diamidino 2 Phenylindole Dihydrochloride), supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pmc02949679-85-24-28
Average 90 stars, based on 1 article reviews

dapi solution (4,6-diamidino-2-phenylindole dihydrochloride) - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells"

Article Title: Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells

Journal: Radiation Oncology (London, England)

doi: 10.1186/1748-717X-5-82

Figure Legend Snippet: PARP protein levels and apoptosis rate of either non-irradiated or irradiated cells after siRNA transfection . A . Western blot analysis of PARP with rabbit anti-human cleaved PARP (Asp214) antibody in MDA-MB-321 cells 24 h and 72 h after transfection. The cells were untreated (UT), transfected with 100 nM of either nonsense siRNA (non) or target-specific siRNAs to knockdown OPN (Mix and OpnS). The Western blot shows one representative result out of three independent experiments. Actin served as an internal loading control. B . The morphology of DAPI stained cell nuclei was analyzed to quantify the apoptosis rate of MDA-MB-231 cells 72 h after transfection. The diagram shows the apoptosis rate of the cells as a function of treatment and irradiation. A fluorescence microscope was used and 500 cells in several fields of view were counted for each experiment. Data represent the average values (± SD) of three independent experiments (* p < 0.05, ** p < 0.001).

Techniques Used: Irradiation, Transfection, Western Blot, Knockdown, Control, Staining, Fluorescence, Microscopy

Microscopy:

Article Title: Effects of osteopontin inhibition on radiosensitivityof MDA-MB-231 breast cancer cells
Article Snippet: .. The cells were fixed with 80% ethanol (Merck, Darmstadt, Germany) and centrifuged on microscope slides at 1000 g for 5 min. After staining with DAPI solution (4,6-diamidino-2-phenylindole dihydrochloride) (Serva, Heidelberg, Germany) and washing with PBS, the cells were covered with ProLong® Gold antifade reagent (Invitrogen). .. The rate of apoptosis was quantified with a fluorescent microscope at 200× magnification (MC 100 Spot, Zeiss universal microscope, Jena, Germany) by counting 500 cells in separate visual fields (described in [20]).

Article Title: Influence of Tumor Suppressor p53 Functioning on the Expression of Antioxidant System Genes under the Action of Cytotoxic Compounds.
Article Snippet: The effect of inhibition of the tumor suppressor p53 on the antioxidant system genes expression under the influence of cytotoxic compounds of the platinum group was studied.. It was found that the action of platinum(II) and platinum(IV) complexes induced accumulation of p53 protein with a maximum in 12 h, which was confirmed by an increase in the expression of the P21 gene, the target gene of the p53 protein.. It was shown that the action of platinum complexes activated the expression of catalase and superoxide dismutase 2 genes.

Article Title: Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells
Article Snippet: .. The cells were fixed with 80% ethanol (Merck, Darmstadt, Germany) and centrifuged on microscope slides at 1000 g for 5 min. After staining with DAPI solution (4,6-diamidino-2-phenylindole dihydrochloride) (Serva, Heidelberg, Germany) and washing with PBS, the cells were covered with ProLong ® Gold antifade reagent (Invitrogen). .. The rate of apoptosis was quantified with a fluorescent microscope at 200× magnification (MC 100 Spot, Zeiss universal microscope, Jena, Germany) by counting 500 cells in separate visual fields (described in [ ]).

Article Title: Betulinyl Sulfamates as Anticancer Agents and Radiosensitizers in Human Breast Cancer Cells
Article Snippet: .. The cells were fixed with 80% ethanol (Merck, Darmstadt, Germany) and centrifuged on microscope slides at 1000× g for 5 min. After staining with DAPI solution (Serva, Heidelberg, Germany), the cells were covered with Prolong ® Gold Antifade (Invitrogen). .. Apoptotic cells were determined according to nuclear condensation and fragmentation with a fluorescent microscope at 200× magnification (BZ-8000, microscope, Keyence, Japan).

Staining:

Article Title: Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells
Article Snippet: .. The cells were fixed with 80% ethanol (Merck, Darmstadt, Germany) and centrifuged on microscope slides at 1000 g for 5 min. After staining with DAPI solution (4,6-diamidino-2-phenylindole dihydrochloride) (Serva, Heidelberg, Germany) and washing with PBS, the cells were covered with ProLong ® Gold antifade reagent (Invitrogen). .. The rate of apoptosis was quantified with a fluorescent microscope at 200× magnification (MC 100 Spot, Zeiss universal microscope, Jena, Germany) by counting 500 cells in separate visual fields (described in [ ]).

Article Title: Impact of X-ray Exposure on the Proliferation and Differentiation of Human Pre-Adipocytes
Article Snippet: .. Cells were washed with distilled water after performing Oil Red O staining, and the DAPI staining solution (1 μg/mL, SERVA Electrophoresis, Heidelberg, Germany) was added. ..

other:

Article Title: A novel porcine ex vivo retina culture model for oxidative stress induced by H₂O₂.
Article Snippet: A 0.01μg/ml DAPI solution (Cat. No. 18860.01; SERVA Electrophoresis GmbH, Heidelberg, Germany) was applied for five minutes to stain the nuclei.

Article Title: Mechanism and dynamics of INPP5E transport into and inside the ciliary compartment.
Article Snippet: Biological Chemistry ‘Just Accepted’ Papers are papers published online, in advance of appearing in the print journal.. They have been peer-reviewed, accepted and are online published in manuscript form, but have not been copy edited, typeset, or proofread.. Copy editing may lead to small differences between the Just Accepted version and the final version.

Incubation:

Article Title: Alteration of miRNA expression in early endothelial cells after exposure with sub-lethal sulfur mustard concentrations.
Article Snippet: Background/aim: Sulfur mustard (SM) is known to induce chronic wound healing disorders as well as disturbed endothelial regeneration.. It is known that wound healing as well as endothelial regeneration are controlled by micro-RNA (miRNA).. As nothing is known today about the effect of SM onto miRNA expression we wanted to investigate whether there is an effect of sub-lethal concentrations of SM onto the miRNA expression of endothelial cells.

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