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FUJIFILM dapi solution
Dapi Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pm37584382-142-4-9
Average 90 stars, based on 1 article reviews
dapi solution - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Article Snippet: Chemical compound, drug , DAPI solution , FUJIFILM Wako Pure Chemical Corporation , Cat#340-07971 , .

Staining:

Article Title: Macrophage-like Blood Cells Are Involved in Inter-Tissue Communication to Activate JAK/STAT Signaling, Inducing Antitumor Turandot Proteins in Drosophila Fat Body via the TNF-JNK Pathway
Article Snippet: .. After staining with DAPI solution (#5748, FUJIFILM Wako Pure Chemical, Osaka, Japan), the fixed LG samples embedded in Vector Shield (#H-1000, Vector Laboratories, Newark, CA, USA) were gently flattened under a covered glass to prepare specimens of a constant thickness, as described in [ ]. .. Specimens were observed under a fluorescence microscope (I×81; Olympus Co., Tokyo, Japan) equipped with a CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu, Japan).

Article Title: The minimal endosymbiosis between Spurilla braziliana (Aeolidiidae, Nudibranchia) and Symbiodiniaceae
Article Snippet: After washing with PBS, the samples were cleared using the CUBIC Trial Kit (FUJIFILM Wako Pure Chemical Corporation), according to the protocol provided by the manufacturer. .. During the clearing process, nuclear staining was performed using DAPI solution (FUJIFILM Wako Pure Chemical Corporation). .. Chlorophyll autofluorescence from Symbiodiniaceae and DAPI fluorescence were observed under a fluorescence microscope (KEYENCE BZX-810).

Article Title: Macrophage-like Blood Cells Are Involved in Inter-Tissue Communication to Activate JAK/STAT Signaling, Inducing Antitumor Turandot Proteins in Drosophila Fat Body via the TNF-JNK Pathway.
Article Snippet: .. After staining with DAPI solution (#5748, FUJIFILM Wako Pure Chemical, Osaka, Japan), the fixed LG samples embedded in Vector Shield (#H-1000, Vector Laboratories, Newark, CA, USA) were gently flattened under a covered glass to prepare specimens of a constant thickness, as described in [7]. .. Specimens were observed under a fluorescence microscope (I×81; Olympus Co., Tokyo, Japan) equipped with a CCD Int.

Binding Assay:

Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Article Snippet: .. The dissociated cells were aliquoted into 1 × 105 cells/100 μL with a binding buffer consisting of 10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl2, then added with 5 μL Annexin V (Alexa Fluor 680) conjugates (Cat#A35109, Thermo Fisher Scientific) and 1 μL DAPI solution (Cat#340- 07971, FUJIFILM Wako Pure Chemical Corporation). ..

Real-time Polymerase Chain Reaction:

Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Article Snippet: .. or resource Designation Source or reference Identifiers Additional information Commercial assay or kit FastGene RNA premium kit NIPPON Genetics Cat#FG- 81250 Commercial assay or kit ReverTra Ace qPCR RT kit TOYOBO Cat#FSQ- 101 Commercial assay or kit THUNDERBIRD Probe qPCR Mix TOYOBO Cat#QPS- 101 Commercial assay or kit DNeasy Blood & Tissue Kit QIAGEN Cat#69504 Commercial assay or kit Karyostat Assay arrays Thermo Fisher Scientific Cat#905403 Chemical compound, drug Y- 27632 FUJIFILM Wako Pure Chemical Corporation Cat# HY- 10071 Chemical compound, drug 0.5 M EDTA solution Nacalai Tesque Cat#06894- 14 Chemical compound, drug Annexin V (Alexa Fluor 680) conjugates Thermo Fisher Scientific Cat#A35109 Chemical compound, drug DAPI solution FUJIFILM Wako Pure Chemical Corporation Cat340- 07971 Chemical compound, drug 4% paraformaldehyde in phosphate buffer solution Nacalai Tesque Cat#09154- 85 Chemical compound, drug SB431542 FUJIFILM Wako Pure Chemical Corporation Cat#198- 16543 (10 μM) Chemical compound, drug DMH1 FUJIFILM Wako Pure Chemical Corporation Cat#041- 33881 (10 μM) Continued .. In this study, we used WTC11 (GM25256 from Coriell Institute) (Hayashi et al., 2016), 201B7 (HPS0063 from RIKEN Cell Bank) (Takahashi et al., 2007), 454E2 (HPS0077 from RIKEN Cell Bank) (Okita et al., 2011), 1383D6 (HPS1006 from RIKEN Cell Bank) (Okita et al., 2011), 1231A3 (HPS0381 from RIKEN Cell Bank) (Okita et al., 2011), Ff- I14s04 (CiRA foundation, Kyoto University) (Kitano et al., 2022), and HiPS- NB1RGB (HPS5067 from RIKEN Cell Bank), which were generated from human neonatal skin fibroblast (RCB0222) (Borisova et al., 2022; Shimizu et al., 2022), as healthy donor hiPSC lines.



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Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
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Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ <t>,6-diamidino-2-phenylindole.</t>
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Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

Journal: Reviews in Cardiovascular Medicine

Article Title: Targeting Tumor Necrosis Factor-α Mitigates Glucose Fluctuation-Induced Aortic Valve Fibrosis: Insights From Diabetic Rat Models

doi: 10.31083/RCM42804

Figure Lengend Snippet: Colocalization of CD3, CD68, and JAK1 in the aortic valves of diabetic rat models . (A,B) The coimmunostaining analysis of the HG and GF groups demonstrated the presence of CD3, CD68, and JAK1 proteins, indicating that JAK1 expression is primarily localized in T lymphocytes and macrophages. Notably, this expression pattern could be altered through the inhibition of TNF- α . Scale bar = 50 µm. DAPI, 4 ′ ,6-diamidino-2-phenylindole.

Article Snippet: In addition, the concentrated SABC-POD (Mouse/Rabbit IgG) kit (SA2010, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 488 conjugated AffiniPure goat anti-mouse IgG (H + L) (BA1126, BOSTER Biological Technology Co., Ltd., Wuhan, China), DyLight 594 conjugated AffiniPure goat anti-rabbit IgG (H + L) (BA1142, BOSTER Biological Technology Co., Ltd., Wuhan, China), ethylenediaminetetraacetic acid (EDTA) antigen retrieval solution (AR0023, BOSTER Biological Technology Co., Ltd., Wuhan, China), 4 ′ ,6-diamidino-2-phenylindole (DAPI) staining solution (AR1176, BOSTER Biological Technology Co., Ltd., Wuhan, China), human TNF- α enzyme-linked immunosorbent assay (ELISA) kit (EK0525, BOSTER Biological Technology Co., Ltd., Wuhan, China), and human TGF- β 1 ELISA kit (EK0513, BOSTER Biological Technology Co., Ltd., Wuhan, China) were obtained from Boster, China.

Techniques: Expressing, Inhibition