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dapi staining solution  (Miltenyi Biotec)


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    Miltenyi Biotec dapi staining solution
    Dapi Staining Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+4/DAPI+Staining+Solution/us12286475-517-13-18
    Average 97 stars, based on 94 article reviews
    dapi staining solution - by Bioz Stars, 2026-11
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: After 10 minutes, cells were stained with APC anti-human CD3 Antibody (BioLegend, 300412) and FITC anti-human CD25 Antibody (BioLegend, 302603) for 30 minutes on ice. .. Cells were washed once with 1✕ PBS with 2% FBS, pH 7.4, and finally resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 and analyzed on the BD LSRFortessaTM Cell Analyzer. ..

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Article Title: An Integrin β6-targeted antibody-drug conjugate optimized for intravesical delivery to treat non-muscle invasive bladder cancer
    Article Snippet: PDOs and cells were dissociated with TrypLE (ThermoFisher, 12605010) for 5-10 min at 37°C, then distributed into 96-well V-bottom plates (2 x 105 cells/well). .. Cells were stained with h2A2 primary antibody (1 hour, 4°C), anti-IgG-FITC (45 minutes, 4°C) and DAPI (Miltenyi Biotec, 130-111-570) (5 minutes). .. Samples were acquired within 4 hours on a MACSQuant Analyzer 10 (Miltenyi Biotec, RRID:SCR_020268) or Attune NxT flow cytometer and analyzed using FlowJo v10.8.

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..

    Single Cell:

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Incubation:

    Article Title: Hyaluronic acid and tissue mechanics orchestrate mammalian digit tip regeneration.
    Article Snippet: .. Then, cells were incubated with ready- to- use DAPI (Miltenyi Biotec, 130- 111- 570) for 5 min in 2% FBS and 2.5 mM EDTA in PBS. .. For immunoblotting, adherent cells on hydrogels were inverted onto Parafilm with RIPA buffer (Thermo Fisher Scientific, 89900) containing Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, 78440).

    Magnetic Cell Separation:

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing