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antifade mounting solution  (Beyotime)


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    Structured Review

    Beyotime antifade mounting solution
    Antifade Mounting Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI/pm42869326-74-2-7
    Average 99 stars, based on 33033 article reviews
    antifade mounting solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells.
    Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach.

    Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis.
    Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing DAPI (Beyotime, P0131, China) to protect the fluorescent signal. ..

    Staining:

    Article Title: Zyxin protects against hypertensive nephropathy by restraining FAK/Skp2-CDK8/YAP signaling in tubular epithelial cells.
    Article Snippet: 1 Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Nephrology, Huadong Hospital, Fudan University, Yan’an Road No. 221, Shanghai 200040, China Abstract Background Tubulointerstitial fibrosis and inflammation are hallmark pathological features of hypertensive nephropathy (HN), yet the underlying molecular mechanisms remain elusive.. Zyxin, a focal adhesion protein, has been implicated in hypertension-related pathologies, but its specific role in HN-induced tubulointerstitial injury remains undefined.. Methods An HN murine model was established via a multi-hit DOCA/Ang II + uninephrectomy + high salt approach.

    Article Title: Sex-Stratified Characterization of Behavioral, Hippocampal, and Microglial Androgen Responses to Adolescent High-Fat Diet Consumption in Mice.
    Article Snippet: .. Finally, the cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; C1027, 1:600; Beyotime, Shanghai, China). ..

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Article Title: Platelet-derived microparticles induce CD8 + T cell senescence in chronic kidney disease via the transfer of microRNA-550a-5p.
    Article Snippet: .. Nuclei were stained with DAPI (Beyotime). .. Visualization was performed using a Zeiss LSM800 NLO confocal microscopy (Carl Zeiss).

    Glass:

    Article Title: HOXA3 Knockdown Impairs hESC-Derived Thymic Epithelial-like Cell Differentiation and CXCL12-Associated Early T-Lineage Support.
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, Biotechnology, Shanghai, China) for 10 min at room temperature in the dark, and coverslips were mounted onto glass slides using an anti-fade mounting medium (Beyotime, China). .. Images were acquired using a Zeiss 880 multiphoton laser scanning microscope (Zeiss, Oberkochen, Germany) or a Nikon single-photon confocal microscope (Nikon, Tokyo, Japan).

    Fluorescence:

    Article Title: SERINC3 promotes oral squamous cell carcinoma progression and mitochondrial metabolic reprogramming involving c-MET/JNK/PGC-1α axis.
    Article Snippet: .. Cells were incubated with the working solution (100 nM) at 37°C for 20 min and fixed with 4% paraformaldehyde for 20 min. Then cells were washed again with PBS and treated with a fluorescence quenching agent containing DAPI (Beyotime, P0131, China) to protect the fluorescent signal. ..

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Confocal:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Microscopy:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: The following day, Alexa Fluor 488-labeled goat anti-mouse IgG (Abcam, ab150113) or Alexa Fluor 594-labeled goat anti-rabbit IgG (Abcam, ab150080) was added and incubated for 1 h in the dark. .. Finally, nuclei were stained with DAPI (Beyotime, C1006) for 5 min, and images were captured using a confocal fluorescence microscope (Leica, Germany). ..

    Modification:

    Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes.
    Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs).

    Saline:

    Article Title: Multimodal Programming of Extracellular Vesicles via Nano-Primed SECCS-Based 3D Microenvironments for Coordinated Ferroptosis Suppression and Myogenic Regeneration in Diabetes.
    Article Snippet: Chronic diabetic glucolipotoxicity is associated with progressive skeletal muscle atrophy, bone loss, cellular senescence, and ferroptotic injury.. Because ferroptosis inhibition alonemay not restore compromisedmyogenic capacity, we developed a combined stem cell-conditioning strategy integrating fullerol priming with the stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS).. This integrated three-dimensional culture platform uses porous β-TCP granules and intermittent medium circulation to generate BMSCs-derived extracellular vesicles (EVs).



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing