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dapi  (Dojindo Labs)


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    Structured Review

    Dojindo Labs dapi
    Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/Cellstain+DAPI+solution/pmc13636658-94-53-54
    Average 96 stars, based on 489 article reviews
    dapi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: Primary and secondary antibodies used were rabbit polyclonal anti-phospho-S6K (1:500, #9209, Cell Signaling Technology), rabbit monoclonal anti-phospho-Akt (1:500, #4060, Cell Signaling Technology), rabbit monoclonal anti-Akt (1:500, #4691, Cell Signaling Technology), goat polyclonal anti-GAPDH (1:500, IMG-3073, Imgenex), HRP-linked anti-rabbit IgG (1:2,000, #7074, Cell Signaling Technology), and HRP-linked anti-goat IgG (1:2,000, ab97110, Abcam).

    Staining:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Correlation-Based Morphometric Analysis Reveals Structural Remodeling of Platelet-Bound Circulating Tumor Cells in Urothelial Carcinoma.
    Article Snippet: Circulating tumor cells (CTCs) mediate hematogenous metastasis and are key targets of liquid biopsy.. Although platelet−tumor cell interactions enhance survival and dissemination, systematic studies of CTCs remain limited because of their rarity and heterogeneity.. We report a single-cell approach for evaluating platelet-bound CTCs.

    Incubation:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Blocking Assay:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Cell-Signaling:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Modification:

    Article Title: 4-Phenylbutyric acid activates an NF-κB-Egr-1 axis to control myoblast proliferation and ECM gene expression.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER cOmplete ULTRA Tablets, Mini, EDTA-free, EASYpack Roche Cat #: 5892791001 DAPI (4′,6-diamidino-2-phenylindole) Solution Dojindo Cat #: D523 Dimethyl sulfoxide (DMSO) Nacalai tesque Cat #: 13408-64 (−)-DHMEQ MedChemExpress Cat #: HY-14645 Dulbecco’s Modified Eagle Medium (DMEM), high glucose Nacalai tesque Cat #: 08459-64 Ethanol FUJIFILM Wako Pure Chemical Corporation Cat #: 054-07225 Fetal bovine serum (FBS) (Lot. .. 0001641439) Nichirei Biosciences Cat #: 173012-500ML Glycine Nacalai tesque Cat #: 17109-35 Hanks’ Balanced Salt Solution/HBSS Nacalai Tesque Cat #: 17461-05 HCl FUJIFILM Wako Pure Chemical Corporation Cat #: 084-05425 Horse serum (HS) GIBCO Cat #:26050088 Immobilon Western Chemiluminescent HRP Substrate Cytiva Cat #: RPN2235 Lipofectamine RNAiMAX transfection reagent Invitrogen Cat #: 13778075 Hilymax Dojindo Cat #: H357 M-PER Mammalian Protein Extraction Reagent Thermo Fisher Cat #: 78501 Matrigel Corning Cat #: 354234 NaCl FUJIFILM Wako Pure Chemical Corporation Cat #: 191-01665 Paraformaldehyde (37% PFA, for crosslinking) FUJIFILM Wako Pure Chemical Corporation Cat #: 063-04815 Paraformaldehyde (4% PFA) FUJIFILM Wako Pure Chemical Corporation Cat #:163-20145 Penicillin-Streptomycin Mixed Solution Nacalai tesque Cat #: 26253-84 Phosphatase Inhibitor Cocktail Solution II(×100) FUJIFILM Wako Pure Chemical Corporation Cat #: 160-24371 Phosphate-buffered saline (PBS, pH 7.4) Nacalai tesque Cat #: 14249-24 ProLong Glass Antifade Mountant Thermo Fisher Cat #: P36965 Protein A Mag Sepharose Cytiva Cat #: 28944006 PicoLab Rodent Diet 20 LabDiet Cat #: 5053 Red Blood Cell (RBC) Lysis Buffer/RCK buffer BioLegend Cat #: 420301 Sample Buffer Solution with Reducing Reagent(6×) for SDS-PAGE pH6.8 Nacalai tesque Cat #: 09499-14 Sodium Dodecyl Sulfate FUJIFILM Wako Pure Chemical Corporation Cat #: 199-07141 Tauroursodeoxycholic Acid (TUDCA) Selleck Biotech Cat #: S3654 Triton X-100 Sigma-Aldrich Cat #: 9002-93-1 Trypsin-EDTA (0.25%) Nacalai tesque Cat #: 35554-64 Tunicamycin Cayman Chemical Cat #: 11445 Tween 20 Sigma-Aldrich Cat #: P9416-100ML Silencer Select Mouse Hdac5 siRNA (Assay ID s67423) Invitrogen Cat #: 4390771 Silencer Select Mouse Egr-1 siRNA (Assay ID s65378) Invitrogen Cat #: 4390771 Silencer Select Negative Control No. 1 siRNA Invitrogen Cat #: 4390843 Critical commercial assays Agilent 4200 TapeStation D1000 assay Agilent Cat #: D1000 CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) Promega Cat #: G3582 ISOSPIN Cell & Tissue RNA kit Nippon Gene Cat #: 314-08211 JC-1 MitoMP Detection Kit Dojindo Cat #: MT09 Maxwell RSC Simply RNA Cell Kit Promega Cat #: AS1390 (Continued on next page) iScience 29, 117620, October 16, 2026 17



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