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dapi  (Dojindo Labs)


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    Structured Review

    Dojindo Labs dapi
    Dapi, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/Cellstain+DAPI+solution/pm42835118-260-15-18
    Average 96 stars, based on 492 article reviews
    dapi - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: A CD4 + T cell-fibroblast crosstalk exacerbates autoimmunity in a mouse model of primary Sjögren disease.
    Article Snippet: To evaluate nuclear translocation of NF-κB p65, fibroblasts (1×105 cells/well) from WT- or Tnfrsf8-deficient pSjD model mouse SG were first AR TI CL E IN P RE SS cultured in DMEM containing 10% FBS on collagen coated-culture cover glass (Matsunami Glass Ind., Ltd., Cat# CS1163) placed in a 24-well plate for one day.

    Article Title: Inter-organ metabolic feedback via BCAA catabolism regulates glucagon-like hormone secretion in Drosophila.
    Article Snippet: Primary and secondary antibodies used were rabbit polyclonal anti-phospho-S6K (1:500, #9209, Cell Signaling Technology), rabbit monoclonal anti-phospho-Akt (1:500, #4060, Cell Signaling Technology), rabbit monoclonal anti-Akt (1:500, #4691, Cell Signaling Technology), goat polyclonal anti-GAPDH (1:500, IMG-3073, Imgenex), HRP-linked anti-rabbit IgG (1:2,000, #7074, Cell Signaling Technology), and HRP-linked anti-goat IgG (1:2,000, ab97110, Abcam).

    Article Title: 4-Phenylbutyric acid activates an NF-κB-Egr-1 axis to control myoblast proliferation and ECM gene expression.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Egr-1 Proteintech Cat #: 55117-1-AP; RRID: AB_2881272 Mouse anti-Fos Proteintech Cat #: 66590-1-IG; RRID: AB_2881950 Rabbit anti-MYH1, clone 1E15 Sigma-Aldrich Cat #: ZRB1214; RRID: AB_3083662 Rabbit anti-MYH7, clone 1B19 Sigma-Aldrich Cat #: ZRB1308 Mouse anti-NCL-Hamlet DYSF Leica Biosystems Cat #: NCL-Hamlet; RRID: AB_442097 Mouse anti-Myogenin BD Biosciences Cat #: 556358; RRID: AB_396383 Rabbit anti-Desmin Proteintech Cat #: 16520-1-AP; RRID: AB_2292918 Rabbit anti-β-Actin GeneTex Cat #: GTX110564; RRID: AB_10618080 Rabbit anti-Phospho- ERK1/2 (Thr202/Tyr204) Proteintech Cat #: 28733-1-AP; RRID: AB_2881202 Rabbit anti-Erk1/2 Proteintech Cat #: 11257-1-AP; RRID: AB_2139822 Rabbit anti-Phospho-NF-κB p65 (Ser468) Proteintech Cat #: 82335-1-RR; RRID: AB_3083091 Rabbit anti-Phospho-NF-kB p65 (Ser536) Cell Signaling Technology Cat #: 3033; RRID: AB_331284 Rabbit anti-Acetyl-NFkB p65 (Lys310) Invitrogen Cat #: PA5-17264; RRID: AB_10983502 Rabbit anti-NF-κB p65 BioLegend Cat #: 622602; RRID: AB_315956 Rabbit anti-Histone H3 (D1H2) Cell Signaling Technology Cat #: 4499; RRID: AB_10544537 Rabbit anti-Acetyl-Histone H3 (Lys9) (C5B11) Cell Signaling Technology Cat #: 9649; RRID: AB_823528 Rabbit anti-Acetyl-Histone H3 (Lys14) (D4B9) Cell Signaling Technology Cat #: 7627; RRID: AB_10839410 Rabbit anti-Acetyl-Histone H3 (Lys18) (D8Z5H) Cell Signaling Technology Cat #: 13998; RRID: AB_2783723 Rabbit anti-Acetyl-Histone H3 (Lys27) (D5E4) Cell Signaling Technology Cat #: 8173; RRID: AB_10949503 Rabbit anti-Acetyl-Histone H3 (Lys56) Cell Signaling Technology Cat #: 4243; RRID: AB_10548193 Rabbit anti-HDAC5 (D1J7V) Cell Signaling Technology Cat #: 20458; RRID: AB_2713973 Rabbit anti-Phospho-eIF2α (Ser51) (D9G8) Cell Signaling Technology Cat #: 3398T; RRID: AB_2096481 Rabbit anti-eIF2α (D7D3) Cell Signaling Technology Cat #: 5324T; RRID: AB_10692650 Rabbit anti-Grp78 GeneTex Cat #: GTX113340; RRID: AB_11173017 Rabbit anti-ATF4 GeneTex Cat #: GTX101943; RRID: AB_1240487 Rabbit anti-GADD153 (CHOP) GeneTex Cat #: GTX112827 Rabbit anti-IRE1 (pSer724) GeneTex Cat #: GTX132808; RRID: AB_2886751 Rabbit anti-IREα (14C10) Cell Signaling Technology Cat #: 3294T; RRID: AB_823545 Mouse anti-ATF6 Antibody (70B1413.1) Novus Biologicals Cat #: NBP1-40256; RRID: AB_2058774 Rabbit anti-Pax7 [EPR30466-531] abcam Cat #: ab324017 Rabbit anti-OPA1 (D6U6N) Cell Signaling Technology Cat #: 80471T; RRID: AB_2734117 Rabbit anti-DRP1 (C-terminal) Proteintech Cat #: 12957-1-AP; RRID: AB_2093525 Mouse anti-PGC1α Proteintech Cat #: 66369-1-IG; RRID: AB_2828002 Goat anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat #: 7074S; RRID: AB_2099233 Horse anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat #: 7076S; RRID: AB_330924 Goat anti-Rabbit IgG H&L (Alexa Fluor 488) abcam Cat #: ab150077; RRID: AB_2630356 Goat anti-Mouse IgG H&L (Alexa Fluor 488) abcam Cat #: ab150113; RRID: AB_2576208 Purified anti-Rabbit IgG BioLegend Cat #: 410404; RRID: AB_2632606 Chemicals, peptides, and recombinant proteins 4-Phenylbutyric acid (4-PBA) Sigma-Aldrich Cat #: P21005-25G Block Ace blocking reagent DS Pharma Biomedical Cat #: UK-B80 Bovine serum albumin (BSA) Nacalai tesque Cat #: 01281-26 Collagenase type 1 Worthington Biochemical Corporation Cat #: CLS1 (Continued on next page) 16 iScience 29, 117620, October 16, 2026

    Staining:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Correlation-Based Morphometric Analysis Reveals Structural Remodeling of Platelet-Bound Circulating Tumor Cells in Urothelial Carcinoma.
    Article Snippet: Circulating tumor cells (CTCs) mediate hematogenous metastasis and are key targets of liquid biopsy.. Although platelet−tumor cell interactions enhance survival and dissemination, systematic studies of CTCs remain limited because of their rarity and heterogeneity.. We report a single-cell approach for evaluating platelet-bound CTCs.

    Incubation:

    Article Title: In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier.
    Article Snippet: .. For samples without antibody staining, the sections were incubated with DAPI (1:500, #D523, Dojindo) in PBS for 10 min, rinsed, mounted in Fluoromount (#K024, Diagnostic BioSystems), and cover-slipped. ..

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Blocking Assay:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.

    Cell-Signaling:

    Article Title: Functional Characterization and Genotype‐Phenotype Correlation of Androgen Receptor Variants in Patients With Androgen Insensitivity Syndrome
    Article Snippet: .. After 48 h, cells were fixed with 4% paraformaldehyde for 15 min and blocked with 0.5% Triton X‐100‐containing blocking buffer for 2 h. Cells were incubated overnight with a primary anti‐AR antibody (Cell Signaling Technology, D6F11), followed by incubation with a fluorescent secondary antibody (Invitrogen, A21428) for 2 h. Nuclei were counterstained with DAPI (Dojindo Laboratories, D523) for 10 min. .. Images were acquired using a confocal laser‐scanning microscope (Zeiss) with a 40× objective.



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy