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mounted with a prolong gold anti-fade mounting solution containing dapi  (Thermo Fisher)


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    Thermo Fisher mounted with a prolong gold anti-fade mounting solution containing dapi
    Mounted With A Prolong Gold Anti Fade Mounting Solution Containing Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+mounting+solution+containing+dapi/pm39424270-117-34-35
    Average 90 stars, based on 1 article reviews
    mounted with a prolong gold anti-fade mounting solution containing dapi - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A modular toolbox for the optogenetic deactivation of transcription
    Article Snippet: After 30 min blocking with 1% BSA, slides were incubated overnight at 4°C with primary antibodies: goat anti GFP (1:400, Abcam), rabbit anti RFP (1:500, Abcam) and mouse anti Cas9 (1:500, Active Motif). .. After incubation with secondary antibodies (donkey anti goat Alexa 488 [1/800, Thermo Fisher], donkey anti rabbit Alexa 568 [Thermo Fisher] and donkey anti mouse Alexa 750 [Abcam]), cells were counterstained with DAPI and mounted with Prolong Gold Antifade Mounting medium (Thermo Fisher). .. Slides were imaged by a Zeiss confocal LSM980, using a 63× oil objective with tile and doing an orthogonal projection of a z-stack of at least 12 layers, 0.7 μm wide each.

    Article Title: Host serine proteases and antiviral innate immunity as potential therapeutic targets in influenza A virus infection-induced COPD exacerbations
    Article Snippet: .. Cells were rinsed with PBS(-/-), fixed with 4% paraformaldehyde (Alfa Aesar) for 20 min at room temperature, permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) in PBS (PBSX) for 10 min, blocked with 5% goat serum (Life Technologies, #50062Z) in PBSX for 1 h at room temperature, and incubated with antibody diluted in blocking buffer (5% goat serum in PBSX) overnight at 4 °C, followed by incubation with fluorescent-conjugated secondary antibody for 1 h at room temperature; nuclei were stained with DAPI (Invitrogen) after secondary antibody staining. .. The following antibodies were used: ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 555, (Invitrogen, #MA3-39100-A555); MUC5AC Monoclonal Antibody (9-13M1), Thermo Fisher, MA1-35708.

    Article Title: Multiomics Analysis Reveals Therapeutic Targets for Chronic Kidney Disease With Sarcopenia
    Article Snippet: .. The myotubes were incubated with fluorescent‐conjugated secondary antibody in the dark for 1 h and then counterstained with DAPI (1:500, Invitrogen, California, USA). .. Finally, the myotubes were photographed with an inverted fluorescence microscope (CarlZeiss, Werk Gottingen, Germany).

    Article Title: MT2A promotes angiogenesis in chronically ischemic brains through a copper–mitochondria regulatory mechanism
    Article Snippet: The primary antibodies used targeted CD31 (Thermo Fisher Scientific, USA), DLAT (Thermo Fisher Scientific, USA) and VDAC1 (Thermo Fisher Scientific, USA). .. Next, the slices were incubated with secondary antibodies with Alexa Fluor 488 (Thermo Fisher Scientific, USA) and Alexa Fluor 555 (Thermo Fisher Scientific, USA) at room temperature for 1 h. The nuclei of HUVECs and paraffin-embedded sections were stained with DAPI (Thermo Fisher Scientific, USA). .. For the determination of mitochondrial function, the cells were incubated with 100 nM MitoTracker Red CMXRos (Thermo Fisher Scientific, USA) for 30 min prior to fixation.

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer's disease.
    Article Snippet: .. To reduce autofluorescence, the sections were incubated in 1 mM CuSO4 diluted in 50 mM ammonium acetate for 15 min. Nuclear staining was with 300 nM DAPI (ThermoFischer Scientific) for 10 min, prior to mounting. .. To visualize amyloid plaques, we used 1 × AmyloGlo stain (Biosensis, Thebarton, Australia) supplemented to the secondary antibody solution.

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
    Article Snippet: .. To reduce autofluorescence, the sections were incubated in 1 mM CuSO 4 diluted in 50 mM ammonium acetate for 15 min. Nuclear staining was with 300 nM DAPI (ThermoFischer Scientific) for 10 min, prior to mounting. .. To visualize amyloid plaques, we used 1 × AmyloGlo stain (Biosensis, Thebarton, Australia) supplemented to the secondary antibody solution.

    Blocking Assay:

    Article Title: Host serine proteases and antiviral innate immunity as potential therapeutic targets in influenza A virus infection-induced COPD exacerbations
    Article Snippet: .. Cells were rinsed with PBS(-/-), fixed with 4% paraformaldehyde (Alfa Aesar) for 20 min at room temperature, permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) in PBS (PBSX) for 10 min, blocked with 5% goat serum (Life Technologies, #50062Z) in PBSX for 1 h at room temperature, and incubated with antibody diluted in blocking buffer (5% goat serum in PBSX) overnight at 4 °C, followed by incubation with fluorescent-conjugated secondary antibody for 1 h at room temperature; nuclei were stained with DAPI (Invitrogen) after secondary antibody staining. .. The following antibodies were used: ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 555, (Invitrogen, #MA3-39100-A555); MUC5AC Monoclonal Antibody (9-13M1), Thermo Fisher, MA1-35708.

    Staining:

    Article Title: Host serine proteases and antiviral innate immunity as potential therapeutic targets in influenza A virus infection-induced COPD exacerbations
    Article Snippet: .. Cells were rinsed with PBS(-/-), fixed with 4% paraformaldehyde (Alfa Aesar) for 20 min at room temperature, permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) in PBS (PBSX) for 10 min, blocked with 5% goat serum (Life Technologies, #50062Z) in PBSX for 1 h at room temperature, and incubated with antibody diluted in blocking buffer (5% goat serum in PBSX) overnight at 4 °C, followed by incubation with fluorescent-conjugated secondary antibody for 1 h at room temperature; nuclei were stained with DAPI (Invitrogen) after secondary antibody staining. .. The following antibodies were used: ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor 555, (Invitrogen, #MA3-39100-A555); MUC5AC Monoclonal Antibody (9-13M1), Thermo Fisher, MA1-35708.

    Article Title: A Mechanically Stimulated Co-culture in 3-Dimensional Composite Scaffolds Promotes Osteogenic and Anti-osteoclastogenic Activity and M2 Macrophage Polarization
    Article Snippet: .. After the last wash, cytoskeletal actin was stained with the TRITC-phalloidin conjugate (Sigma-Aldrich, St. Louis, MO, USA) for 1 h according to the manufacturer’s instructions and cell nuclei were stained with DAPI (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) for 5 min. .. The samples were washed with PBS and observed under a confocal microscope (TCS SP8, Leica).

    Article Title: MT2A promotes angiogenesis in chronically ischemic brains through a copper–mitochondria regulatory mechanism
    Article Snippet: The primary antibodies used targeted CD31 (Thermo Fisher Scientific, USA), DLAT (Thermo Fisher Scientific, USA) and VDAC1 (Thermo Fisher Scientific, USA). .. Next, the slices were incubated with secondary antibodies with Alexa Fluor 488 (Thermo Fisher Scientific, USA) and Alexa Fluor 555 (Thermo Fisher Scientific, USA) at room temperature for 1 h. The nuclei of HUVECs and paraffin-embedded sections were stained with DAPI (Thermo Fisher Scientific, USA). .. For the determination of mitochondrial function, the cells were incubated with 100 nM MitoTracker Red CMXRos (Thermo Fisher Scientific, USA) for 30 min prior to fixation.

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer's disease.
    Article Snippet: .. To reduce autofluorescence, the sections were incubated in 1 mM CuSO4 diluted in 50 mM ammonium acetate for 15 min. Nuclear staining was with 300 nM DAPI (ThermoFischer Scientific) for 10 min, prior to mounting. .. To visualize amyloid plaques, we used 1 × AmyloGlo stain (Biosensis, Thebarton, Australia) supplemented to the secondary antibody solution.

    Article Title: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
    Article Snippet: .. To reduce autofluorescence, the sections were incubated in 1 mM CuSO 4 diluted in 50 mM ammonium acetate for 15 min. Nuclear staining was with 300 nM DAPI (ThermoFischer Scientific) for 10 min, prior to mounting. .. To visualize amyloid plaques, we used 1 × AmyloGlo stain (Biosensis, Thebarton, Australia) supplemented to the secondary antibody solution.



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