4',6-diamidine-2-phenylindole (dapi) counterstain solution (Millipore)
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4',6-diamidine-2-phenylindole (dapi) counterstain solution
4',6 Diamidine 2 Phenylindole (Dapi) Counterstain Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+counterstain+solution/dapi/pmc04135227-120-19-23
Average 90 stars, based on 1 article reviews
4',6 Diamidine 2 Phenylindole (Dapi) Counterstain Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+counterstain+solution/dapi/pmc04135227-120-19-23
Average 90 stars, based on 1 article reviews
4',6-diamidine-2-phenylindole (dapi) counterstain solution - by Bioz Stars,
2026-11
90/100 stars
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![Activation of Wnt/β-catenin signaling enhances STB differentiation. (A) A scheme of the culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing dbcAMP [CHIR(−) cAMP and CHIR(+) cAMP] for another 6 days. (B) The relative expression level of a vCTB marker, TEAD4 . (C) Phase contrast images of cells on day 9. Multinucleated STB is outlined by a red line. Scale bar = 100 μm. (D, G) The relative expression levels of STB (D) and EVT (G) markers. For C, D, and G, the mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those in CHIR(−) (arbitrarily set as 1). Different letters represent significant differences ( p < 0.05). (E) Detection of multinucleated STBs by immunofluorescence for E-cadherin, CG, and <t>DAPI</t> staining. Scale bar = 100 μm. (F) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs. (H) Detection of gelatinase activities of MMP2 and MMP9 by gelatin zymography.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7102/pmc11647102/pmc11647102__ioae131f2.jpg)
![macTSCs acquire FSK responsiveness for STB differentiation by Wnt/β-catenin signaling activation. (A) A scheme of the macTSC culture conditions. Undifferentiated macTSCs were cultured with [CHIR(+)] or without [CHIR(−)] CHIR99021 for 3 days. Some cells were induced to differentiate in the medium containing forskolin (FSK) [CHIR(−) FSK and CHIR(+) FSK] for another 6 days. (B) Phase contrast images of cells on day 9. Multinucleated STB is indicated by a red line. Scale bar = 100 μm. (C) The relative expression levels of STB markers. The mean values (±SD) of technical triplicates of biological duplicates, normalized by the expression of ACTB , are shown relative to those of cells maintained under the CHIR(−) condition (arbitrarily set as 1). (D) Detection of multinucleated STB by immunofluorescence for E-cadherin, CG, and DAPI staining. Scale bar = 100 μm. (E) The mean intensity ± SD (%) of STB nuclei calculated from four randomly chosen fields of biological duplicates. Different letters represent significant differences ( p < 0.05). Note that error bars got larger as the STB ratio increased, likely because the larger the STBs formed, the sparser the distribution of STBs became so that some of the randomly selected fields contained very few STBs.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7102/pmc11647102/pmc11647102__ioae131f3.jpg)