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cuvette  (Nepa Gene Co Ltd)


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  • 99

    Structured Review

    Nepa Gene Co Ltd cuvette
    Cuvette, supplied by Nepa Gene Co Ltd, used in various techniques. Bioz Stars score: 99/100, based on 2302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cuvette/NEPA21/pm42847431-58-7-9
    Average 99 stars, based on 2302 article reviews
    cuvette - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Electroporation:

    Article Title: Chlamydomonas γ-tubulin mutations reveal a critical role for γ-TuRC in maintaining the stability of centriolar microtubules.
    Article Snippet: .. These plasmids were introduced into the wild-type, bld13-1 or bld13-2 cells by electroporation using the NEPA21 electroporator (Nepa Gene, Japan) (Yamano et al., 2013). .. Of the transformants that display resistance to hygromycin, the clones that express the HA-tagged γ-tubulin were screened by Western blotting using an anti-HA antibody (3F10, Roche).

    Article Title: Patient-derived teratomas as a humanized platform for dystrophin restoration by ex vivo cell therapy and in vivo base editing.
    Article Snippet: Y27632 (10 μM; PeproTech #1293823) was used for 1 day after the attachment if needed. .. According to the standardized protocol for electroporation of human pluripotent stem cells28, gene delivery with the electroporator (NEPA-21, NEPAGENE), iPS cells were rinsed with DPBS and detached with Accutase (#561527, BD Bios-ciences). .. Cells were resuspended and diluted to 1 × 106 cells per 100 μl with Opti-MEM (#31985070, Gibco) after three rounds of Opti-MEM washing (#31985070, Gibco).

    Article Title: Discovery of Avian TCRVβ2 + T Cells Recognizing the Endogenous Ganglioside via a chCD1.1‐Dependent Pathway
    Article Snippet: .. shRNAs targeting selected genes were designed using siRNA Wizard software, cloned into psiRNA‐EGFP‐Zeo vectors (InvivoGen), and transfected into splenocytes 24 h before CpG‐ODN stimulation by electroporation using a NEPA21 electroporator (Nepa Gene Co., Ltd., Chiba, Japan). ..

    Article Title: A PATHOGENIC NFKB2 VARIANT CAUSES COMBINED IMMUNODEFICIENCY WITH THYMIC AND PERIPHERAL LYMPHOID DEFECTS IN RATS
    Article Snippet: .. Electroporation was performed using the NEPA21 system (NEPA GENE Co. Ltd, Sonidel, Ireland) as previously described [ ]. ..

    Platinum:

    Article Title: Phosphorylation-dependent control of transcription factor activity regulates temporal patterning of cortical cell fate specification.
    Article Snippet: .. The plasmids were electroporated into the dorsal ventricular zone with six pulses of 30V, 50ms each at 1 s interval delivered through platinum electrodes using round electrodes CUY650P5 and Nepa Gene electroporator (NEPA21 Type II). ..

    Incubation:

    Article Title: DND1–NANOS3 shapes the PGC transcriptome via a heptanucleotide sequence in mRNA 3′-UTRs
    Article Snippet: A single-strand oligonucleotide donor was purchased from IDT (CTGTCATCTGGACTTTTCTTAATTTCCTACGTATAACTTAATTAACATGTAAATATTATTCCAT GCC A C T C TAAATATAATTCTGTATATGTGCAGATGTCACTGTGGTGGCTGTTAATTACTATAACACAAGTG; the five underlined nucleotides in bold are mutated). .. The sgRNA was first incubated with Cas9 protein (IDT) to form Cas9-sgRNA RNP complex, which was then co-electroporated with the oligo template into zygotes collected from C57BL/6 mice using a Nepa21 electroporator (Nepa Gene Co.) following previously described procedures 94 . .. The electroporated zygotes were cultured overnight in KSOM medium (Millipore Sigma) at 37°C with 6% CO 2 .

    Software:

    Article Title: Discovery of Avian TCRVβ2 + T Cells Recognizing the Endogenous Ganglioside via a chCD1.1‐Dependent Pathway
    Article Snippet: .. shRNAs targeting selected genes were designed using siRNA Wizard software, cloned into psiRNA‐EGFP‐Zeo vectors (InvivoGen), and transfected into splenocytes 24 h before CpG‐ODN stimulation by electroporation using a NEPA21 electroporator (Nepa Gene Co., Ltd., Chiba, Japan). ..

    Clone Assay:

    Article Title: Discovery of Avian TCRVβ2 + T Cells Recognizing the Endogenous Ganglioside via a chCD1.1‐Dependent Pathway
    Article Snippet: .. shRNAs targeting selected genes were designed using siRNA Wizard software, cloned into psiRNA‐EGFP‐Zeo vectors (InvivoGen), and transfected into splenocytes 24 h before CpG‐ODN stimulation by electroporation using a NEPA21 electroporator (Nepa Gene Co., Ltd., Chiba, Japan). ..

    Transfection:

    Article Title: Discovery of Avian TCRVβ2 + T Cells Recognizing the Endogenous Ganglioside via a chCD1.1‐Dependent Pathway
    Article Snippet: .. shRNAs targeting selected genes were designed using siRNA Wizard software, cloned into psiRNA‐EGFP‐Zeo vectors (InvivoGen), and transfected into splenocytes 24 h before CpG‐ODN stimulation by electroporation using a NEPA21 electroporator (Nepa Gene Co., Ltd., Chiba, Japan). ..



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