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Arrayit Corporation customized spotbot3 microarrayer
Customized Spotbot3 Microarrayer, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/customized+microarrayer/spotbot3+microarray+protein+edition/us09599591-528-15-20
Average 90 stars, based on 1 article reviews
customized spotbot3 microarrayer - by Bioz Stars, 2026-09
90/100 stars

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Article Title: New Cattle Genotyping System Based on DNA Microarray Technology
Article Snippet: A prototyping model of the DNA microarray system for identification of genetic markers related to productivity, determination of cattle breed purity, and identification of cattle monogenic diseases was developed.. A method for immobilizing oligonucleotides on a polymer support, their fixation using ultraviolet irradiation, and DNA hybridization to them, followed by labeling and genotype determination, was developed.. Genotyping of two genes encoding milk caseins, i.e., CSN3 kappa-casein gene and CSN2 beta-casein gene, in the Aberdeen Angus breed using real-time PCR and the developed DNA microarray showed identical results.

Article Title: Serum Epiplakin Might Be a Potential Serodiagnostic Biomarker for Bladder Cancer
Article Snippet: Serum epiplakin levels were detected using an automated micro-dot blot array spotBot3 (Arrayit Corp., Sunnyvale, CA, USA).

Article Title: Electrochemical distinction of neuronal and neuroblastoma cells via the phosphorylation of the cellular extracellular membrane.
Article Snippet: In this contribution we establish a proof of concept method for monitoring, quantifying and differentiating the extracellular phosphorylation of Human SHSY5Y undifferentiated neuronal cells and neuroblastoma cells by three prominent ectokinases PKA, PKC and Src.. Herein it is demonstrated that a combination of different experimental techniques, including fluorescence microscopy, quartz crystal microscopy (QCM) and electrochemistry, can be used to detect extracellular phosphorylation levels of neuronal and neuroblastoma cells.. Phosphorylation profiles of the three ectokinases, PKA, PKC and Src, were investigated using fluorescence microscopy and the number of phosphorylation sites per kinase was estimated using QCM.

Article Title: Consecutive Silver(I) Ion Incorporation into Oligonucleotides containing Cytosine-Cytosine Mispairs.
Article Snippet: Herein, the consecutive incorporation of Ag ion into the dsDNA containing adjacent C C mispairs is demonstrated.. The melting temperature (Tm) was 8 °C higher for DNA containing three C C mispairs upon the addition of three Ag ions as compared to the Ag-free DNA, and no Tm was obtained in the presence of excess Ag ion, indicating a stable bridging of C-Ag C upon the incorporation of the stoichiometric amount of Ag per C C mispair.. The circular dichroism (CD) spectra of the dsDNA showed a negative peak at ~270 nm in the presence of excess Ag, implying that significant structural changes and a potential aggregation of DNA occurred.

Article Title: Microfluidic device
Article Snippet: Robotic systems such as Arraylt SpotBot3 (Arrayit) and MicroGrid II Microarrayer (BioRobotics) have been successfully used for printing microarrays when a wide range of conditions were tested or when printing parameters have been finalized where high spot density and uniform morphology were required.

Microarray:

Article Title: Analysis of serum glycome by lectin microarrays for prostate cancer patients - a search for aberrant glycoforms.
Article Snippet: This is the first work focused on glycoprofiling of whole Nand Oglycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients.. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant bacterial lectins and 3 human lectins (17 lectins in total).. Clinical validation of glycans as biomarkers was done in two studies: discrimination of healthy individuals with BPH patients vs. PCa patients (C vs. PCa) and discrimination of healthy individuals vs. BPH and PCa patients (H vs. PCond).

SPR Assay:

Article Title: Small Molecule SHP2 Inhibitor LXQ-217 Affects Lung Cancer Cell Proliferation in Vitro and in Vivo.
Article Snippet: Background: SHP2 is highly expressed in a variety of cancer and has emerged as a potential target for cancer therapeutic agents.. The identification of uncharged pTyr mimics is an important direction for the development of SHP2 orthosteric inhibitors.. Methods: Surface plasmon resonance analysis and cellular thermal shift assay were employed to verify the direct binding of LXQ-217 to SHP2.

Article Title: Allosteric inhibition of PTP1B by bromocatechol-chalcone derivatives.
Article Snippet: Development of allosteric inhibitors may be a viable strategy to discover hypoglycemic drugs targeting PTP1B.. Allosteric inhibitors occupying the BB site that is a hydrophobic pocket restrict the WPD loop in an open conformation, preventing the physiological dephosphorylation reaction.. Toward the BB site, sixty bromocatechol-chalcone derivatives were designed and synthesized as allosteric inhibitors of PTP1B against diabetes mellitus.



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Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized microarrayer and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.

Journal: Acta biomaterialia

Article Title: Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications

doi: 10.1016/j.actbio.2016.09.006

Figure Lengend Snippet: Schematic representation of fabrication of peptide-functionalized PEG hydrogel microarrays. A. The printing solutions composed of PEGDA monomer and various methacrylated peptides were prepared in a 384-well plate. B. The printing solutions were placed onto poly(HEMA) coated microscope slides with a customized microarrayer and polymerized by UV under Argon protection to prepare peptide-functionalized PEG hydrogel spots. Eight hydrogel spots in a microarray were shown to present the dimension of the hydrogel spots and the distance between the hydrogel spots. C. High throughput analysis of cellular activities after cell seeding onto the microarray.

Article Snippet: The microarrays were printed in a humid Ar-atmosphere on epoxy monolayer-coated glass slides (Xenopore XENOSLIDE E, Hawthorne, NJ) that were first dip-coated in 4 v/v% poly(hydroxyethyl methacrylate) (i.e., poly(HEMA)) using a customized microarrayer (Biodot).

Techniques: Microscopy, Microarray, High Throughput Screening Assay